The effect of a sulfonated shale oil extract (ichthyol) on the migration of human neutrophilic granulocytes in vitro

Abstract

The sulfonated shale oil extract, Ichthyol, was studied for its effect on the migration of human neutrophilic granulocytes by the Boyden chamber technique. When presented to the cells in a concentration gradient, Ichthyol induced a directed migration. There was little or no chemokinetic effect of Ichthyol when added to the cell compartment of the Boyden chamber. The chemotactic migration towards the tripeptides, formyl-methionyl-leucyl-phenylalanine and formyl-norleucyl-leucyl-phenylalanine, towards the arachidonic acid-derived eosinophil chemotactic factor released from neutrophils by the ionophore A 23187, and towards complement-derived chemotactic activity of normal human serum was inhibited or abrogated by Ichthyol. The Ichthyol effect on f-MLP chemotaxis could be partly overcome by excessive f-MLP concentrations. It was reversible when Ichthyol-incubated cells were washed and resuspended in regular buffer. It is suggested that various substances contained in Ichthyol interacted with either the chemotactic factors or the cell membrane or both and thus blocked cell stimulation. The results could help to explain the cell accumulation and abscess formation observed with Ichthyol in inflammatory skin lesions and the anti-inflammatory properties of the drug.

Anti-inflammatory action of pale sulfonated shale oil (ICHTHYOL pale) in UVB erythema test

“…The pronounced anti-erythematous effect of 4% PSSO is in accordance with the results of a series of experimental studies which verify a definite anti-inflammatory efficacy [9][10][11][12].…”

“…An influence on leukocyte arachidonic acid metabolites seems likely. Inhibition of the release of chemotactic arachidonic acid metabolites (LTB 4 ) from human leukocytes and inhibition of neutrophil chemotactic migration have been noted [9,10]. Significant inhibition of the edema induced by application of croton oil to mouse ears could be demonstrated [11].…”

Adherence-induced enhancement of the oxidative burst of human neutrophilic granulocytes: Effects of the surface coat and of divalent cations

“…By amino acid analysis MOC was identical with IL-8. In our hands every single substance capable of inducing the directed migration of PMN increased their adhesiveness and this included the platelet-activating factor (PAF), kallikrein, sulfonated shale oil [10,11] or the detergent sodium dodecyl sulfate. PMN adhesiveness is also increased by the cytokines TNF and GM-CSF which at the same time are potent inhibitors of chemotactic migration [12,13].…”

Longitudinale Untersuchung zur Ausscheidung von 1-Hydroxypyren im Urin nach externer Behandlung mit Steinkohlenteer

“…In Tiervesuchen an Ratten [21] und durch Untersuchungen von Patienten, die aus therapeutischen Gründen mit teer-und damit PAH-haltigen Externa behandelt wurden [4,[21][22][23][24][25][26][27][28][29][30][31][32][33] konnte gezeigt werden, daß PAH nicht nur inhalativ, sondern auch durch die Resorption über die Haut aufgenommen werden. Da die von den PAH produzierten Metabolite, z.B.…”

“…Der Wirkmechanismus des inhomogenen und inkonstanten Steinkohlenteergemisches beruht wahrscheinlich auf einer Verminderung der Anzahl ATPase-positiver Zellen über eine Beeinträchtigung der antigenpräsentie-renden Funktion von Langerhans-Zellen, die Inhibition von Esterasen, die Hemmung der Granulozytenmigration sowie eine Reduzierung der Leukotrien B 4 -Freisetzung [5,6,24].…”