The effect of a new feed additive of humic nature on morphometric indicators of laboratory rats depending on the dose

Abstract

The study of the effects of natural biologically active substances, manifestations and consequences of their application to different species of animals or under different conditions is being conducted. Peculiarities of the implementation of such effects when used separately or in combination, in particular, humic acids with mineral or organic acids, creates opportunities for optimal combination or solution to the complexities of the technological setting of feed additives. Changes in the morphometric parameters of internal organs in rats under the influence of the new feed additive of humic nature «Animal Forte» were detected. The established biochemical changes are confirmed at the histological level. In terms of the degree of expressiveness of the protective effect on the state of the liver parenchyma of rats, the dilution exceeds this dilution. No structural changes were detected in micropreparations of kidneys in animals of groups 2, 3, and 4, full blood and stasis in the capillaries of vascular glomeruli were observed. In the control and fifth experimental groups, the presence of damaged tubules, in which the brush border is rejected, was noted during the examination of kidney histopreparations. The morphometric parameters of the liver underwent the most pronounced changes in animals 1 and 2, intestines -1 and 4, and kidneys and hearts -2 and 5 of experimental groups, respectively. A smaller liver mass was found in the animals of the experimental groups, in particular, a decrease in the liver index in rats of the 1st and 2nd experimental groups by 21 % and 15 %, respectively. Kidneys in animals of experimental groups 2 and 5 had 14 % less weight, but the kidney index did not have significant changes in rats of all experimental groups compared to the control. Almost similar changes were related to heart weight, animals of experimental groups 2 and 3 also had probable changes by 13 %, and 5 groups by 14 %. Intestinal mass was significantly smaller in animals 1 (by 14 %), 3 (by 10 %) and 4 (by 11 %) of experimental groups, but the index was reduced only in animals of experimental group 1 by 16 %.