The Effect of a Fat‐Enriched Diet on the Pattern of Distribution of Pancreatic Islet Cells in the C57BL/6J Mice

Abstract

The C57BL/6J mice are inbred strains and develop the metabolic syndrome of obesity, hyperinsulinemia, hyperglycemia, and hypertension, when fed a high-fat diet. These features are similar to those observed in the human metabolic syndrome. This article examined the effect of fat-enriched (FE) diet on the pattern of distribution of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide (PP)-positive cells in the pancreatic islets of C57BL/6J mice using immunohistochemical methods. Insulin-immunoreactive cells were observed in both the peripheral and central regions of the islets of Langerhans in both FE- and control diet-fed mice. The percentage distribution of insulin-positive cells was similar in FE (83.5 +/- 6.4) compared to control diet-fed C57BL/6J mice (83.8 +/- 6.5). Glucagon-containing cells were discerned in the periphery of pancreatic islets in both FE- and control diet-fed mice. The percentage distribution of glucagon was not statistically different in mice fed with FE (9.9 +/- 2.7) compared to control diet (11.3 +/- 4.9). Somatostatin-positive cells were seen in the outer part of the islet of Langerhans and constitute 12.1% (+/-6.3) and 10% (+/-5.5) of pancreatic islet cells in FE- and control diet-fed mice, respectively. PP-immunoreactive cells were observed in the peripheral region of the pancreatic islets of both FE- and control diet-fed mice. The percentage distribution of PP-positive cells was significantly (2.0 +/- 1.2) lower compared to control (5.1 +/- 2.4). In conclusion, the number of PP is significantly reduced in FE diet-fed mice and may play a role in the pathogenesis of diet-induced metabolic syndrome in C57BL/6J mice.

A Mouse Model of Metabolic Syndrome: Insulin Resistance, Fatty Liver and Non-Alcoholic Fatty Pancreas Disease (NAFPD) in C57BL/6 Mice Fed a High Fat Diet

“…Adeghate and co-workers found a similar percentage of insulin-positive (around 80%) and glucagon-positive cells (around 10%) in C57BL/6 mice fed fat-enriched diet compared to mice fed control diet [45]. It is also possible to demonstrate this effect by analyzing the density of immunolabeled cells, as measured by image analysis.…”

Left ventricle structural remodelling in the prediabetic Goto-Kakizaki rat

“…Transmural sections of the LV free wall were collected to encompass epi-endo-and mid-myocardial layers. These were paraffin embedded for light microscopy or Epon embedded for electron microscopy, employing routine techniques described at length elsewhere (Adeghate et al 2006). Additionally, a subset of hearts was sectioned transversely parallel to the atrioventricular groove for measurement of LV wall thickness.…”

Gastrointestinal system

“…Within the pancreas the number and size of islets of Langerhans varies between regions (Fig 3.39), with physiological state, disease (diabetes) and with age (Adeghate et al 2006). Larger islets tend to be seen close to nutrient arteries and ducts, so consistent sampling is important to avoid overinterpretation of variation in islet size (Kilimnik et al 2009;Hornblad et al 2011).…”