The effect of 5-hydroxytryptamine on intestinal motor function in man

Hendrix, Thomas R.; Atkinson, Michael; Clifton, James; Ingelfinger, Franz J.

doi:10.1016/0002-9343(57)90298-x

Cited by 104 publications

(43 citation statements)

References 16 publications

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“…It was therefore, at first, surprising to find that in vivo the peristaltic activity of a loop of intestine, having its normal blood supply, was scarcely affected by intraluminal application of 5-HT. This agrees, however, with observations in man by Hendrix, Atkinson, Clifton & Ingelfinger (1957), who found that introduction of 5-HT into the lumen of the jejunum had no effect. In our experiments on guinea-pigs concentrations of 5-HT from 10-8 to 10-5 in the fluid passing through the lumen of the intestine were usually without effect or they diminished propulsive activity.…”

Section: X-0supporting

confidence: 92%

The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs

Bülbring¹,

Crema²

1959

The Journal of Physiology

105

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Section: X-0supporting

confidence: 92%

The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs

Bülbring¹,

Crema²

1959

The Journal of Physiology

105

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“…Using the isolated guinea-pig ileum, 5-HT applied to the mucosal or serosal surface causes respectively either a facilitation or an inhibition of the peristaltic reflex (Biilbring & Crema, 1958). In in vivo experiments, a stimulant action of exogenous 5-HT on intestinal motility has been documented in the dog (Ormsbee et al, 1981) and in the guinea-pig (Bilbring & Crema, 1959), whereas human studies have shown both a stimulant and an inhibitory effect on motility (Hendrix et al, 1957;Schmid & Kinzlmeier, 1959). Four major subtypes of 5-HT receptors exist within the gastrointestinal tract (5-HTI, 5-HT2, 5-HT3 and 5-HT4) and have been shown to mediate contraction or relaxation responses in various isolated tissue preparations obtained from the guinea-pig and rat (Bradley et al, 1986;Costall & Naylor, 1990; Dhasmana et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

5‐HT₃ and 5‐HT₄ receptor‐mediated facilitation of the emptying phase of the peristaltic reflex in the marmoset isolated ileum

Tuladhar

Costall

Naylor

1996

British J Pharmacology

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1 The patterns of intestinal motility and the effect of an increase in intraluminal pressure were studied in vitro on segments obtained from the marmoset small intestine. 2 Segments obtained from the distal half of the marmoset small intestine exhibited segmentation, consisting of narrow annular contractions (lasting for 2-3 s) interposed between the relaxed segments of varying length. The subsequent contractions occurred slightly distal to the previous contraction, with ring-like contractions appearing to move in the aboral direction. Such segmentation was infrequent or absent in the segments obtained from the proximal half of the small intestine. An increase in intraluminal pressure inhibited segmentation and finally produced peristalsis in most of the tissues. 3 The influence of 5-hydroxytryptamine (5-HT) receptor agonists and antagonists on the threshold of the peristaltic reflex was investigated in the segments obtained from the distal half of the intestine after segmentation had subsided. The effect of drug application to the serosal surface was measured as a change in threshold pressure required to trigger the peristaltic reflex. A facilitation was defined in two ways (a) as a reduction in the threshold pressure required to trigger the reflex and (b) in those tissues that failed to respond with peristalsis on raising intraluminal pressure to the maximum attainable (1 kPa), as a reduction in threshold pressure compared to this value. 4 5-HT (7.85+0.19), 5-methoxytryptamine (7.79+0.24), 5-carboxamidotryptamine (6.66+0.13) and 2-methyl-5-HT (6.24+0.16) caused a concentration related facilitation of the peristaltic reflex, the pD2 values (mean+s.e.mean) being shown in parentheses. 5 The concentration-response curves to both 5-HT and 5-methoxytryptamine were dextrally shifted in a surmountable manner in the presence of GR 113808 (30 nM). pD2 values for 5-HT and 5-methoxytryptamine were significantly decreased to 6.98 + 0.24 and 6.83 + 0.36 respectively in the presence of GR 113808 (30 nM).6 In the presence of a high concentration of (10 jgM) 5-methoxytryptamine the subsequent addition of 2-methyl-5-HT (3-10 -OM) but not 5-methoxytryptamine (10 gM) facilitated peristalsis; the effect of 3 pM 2-methyl-5-HT was significantly decreased by 2 pM ondansetron. 7 It is concluded that the facilitation of the peristaltic reflex in the marmoset intestine induced by 5-HT at submicromolar concentrations involves a 5-HT4 receptor stimulation with an additional 5-HT3 receptor activation at higher concentrations.

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“…It is well known that 5-HT increases the motility of the human small intestine (Hendrix, Atkinson, Clifton & Ingelfinger, 1957;Haverback & Davidson, 1958;Debray & Besancon, 1961) and Daniel, Honour & Bogoch (1960) concluded from an investigation in vivo that 5-HT directly stimulated the smooth muscle cells of the human ileum. Also, there are several reports of the inhibitory effect of 5-HT on the motility of the human distal colon (Fink & Friedman, 1960;Debray & Besancon, 1961).…”

Section: Discussionmentioning

confidence: 99%

THE EFFECT OF 5‐HYDROXYTRYPTAMINE ON THE HUMAN ILEUM AND COLON IN VITRO

Fishlock

Parks

1966

British Journal of Pharmacology and Chemotherapy

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It is well known that 5-hydroxytryptamine (5-HT) causes alimentary smooth muscle to contract. Most regions of the gut show this response although the sensitivity varies. The rat stomach (Vane, 1957), duodenum (Erspamer, 1940) and colon (Gaddum, 1953) all contract in the presence of small concentrations of this amine, and the guinea-pig duodenum, jejunum and ileum also show the same response (Gaddum & Hameed, 1954).The human lower bowel, however, appears to be different. The taenia coli preparation (Bucknell & Whitney, 1964) and the circular muscle of the sigmoid colon (Fishlock & Parks, 1963;Fishlock, 1964) relax in the presence of 5-HT although other regions of the human alimentary tract have been shown to contract under identical experimental conditions (Fishlock, Parks, & Dewell, 1965). This paper describes the effect of 5-HT on both the longitudinal and circular muscle layers of the human terminal ileum and colon and includes evidence as to the site of action of this amine. METHODLongitudinal and circular muscle strips from 12-30 mm long and 1-2 mm wide have been taken at operation from the ileum and colon and immersed in Krebs solution in an isolated organ bath and placed under a tension of usually 1.5 g. The solution has been gassed with 95% 02 and 5% CO2 and maintained at 370 C. Two methods of obtaining muscle strips have been described previously (Fishlock & Parks, 1966). Both were used in this investigation but differences in response and sensitivity of the strips could not be related to the method. All strips consisted of full thickness bowel wall with the mucosa removed. It should be noted that the longitudinal strips of colon were taken through the taenia coli and that the circular muscle strips were taken from between the taeniae where there is a thin investing layer of longitudinal muscle.The preoperative medication and anaesthesia of the patients from whom the strips were taken varied as described previously (Fishlock & Parks, 1966).The following drugs were used: acetylcholine chloride, atropine sulphate, hexamethonium bromide, 5-hydroxytryptamine creatinine sulphate, lysergic acid diethylamide (LSD), mepyramine maleate, procaine hydrochloride and pronethalol hydrochloride. Concentrations refer to the salts. All solutions were made with physiological saline. RESULTS IleumForty-eight circular muscle strips and 15 longitudinal ones were taken from 42 patients. All came from the terminal part of the ileum (last 0.6 m).

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The effect of 5-hydroxytryptamine on intestinal motor function in man

Cited by 104 publications

References 16 publications

The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs

The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs

5‐HT₃ and 5‐HT₄ receptor‐mediated facilitation of the emptying phase of the peristaltic reflex in the marmoset isolated ileum

THE EFFECT OF 5‐HYDROXYTRYPTAMINE ON THE HUMAN ILEUM AND COLON IN VITRO

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The effect of 5-hydroxytryptamine on intestinal motor function in man

Cited by 104 publications

References 16 publications

The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs

The action of 5‐hydroxytryptamine, 5‐hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea‐pigs

5‐HT3 and 5‐HT4 receptor‐mediated facilitation of the emptying phase of the peristaltic reflex in the marmoset isolated ileum

THE EFFECT OF 5‐HYDROXYTRYPTAMINE ON THE HUMAN ILEUM AND COLON IN VITRO

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5‐HT₃ and 5‐HT₄ receptor‐mediated facilitation of the emptying phase of the peristaltic reflex in the marmoset isolated ileum