The effect of 3BS locus of Sumai 3 on Fusarium head blight resistance in Australian wheats

Xie, Guo Qiang; Zhang, M.C.; Chakraborty, Sukumar; Liu, Chunji

doi:10.1071/ea05250

Cited by 20 publications

(11 citation statements)

References 23 publications

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“…should be different between heterozygous and homozygous genotype lines, and further studies should be carried out to elucidate the mechanism of the resistance expression. In contrast to previous reports on dominant inheritance for the 3BS allele in 'Sumai 3' (Xie et al 2007), our results suggested that the expression of the QTL varied with the genetic background. With the association of FHB resistance and snow mold disease resistance, no significant differences in the snow mold disease resistance were detected between the marker genotypes on chromosome 3BS (Table 1), indicating that the FHB resistance and winter hardiness were not linked at the QTL.…”

Section: Evaluation Of Fhb Response and Snow Mold Disease Resistancecontrasting

confidence: 56%

Microsatellite marker-assisted selection of fusarium head blight resistance in backcrossed Japanese winter wheat lines

et al. 2008

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Section: Evaluation Of Fhb Response and Snow Mold Disease Resistancecontrasting

confidence: 56%

Microsatellite marker-assisted selection of fusarium head blight resistance in backcrossed Japanese winter wheat lines

et al. 2008

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“…However, several studies have shown 3BS region to be constantly linked to FHB resistance suggesting that this region may be highly conserved for major CR diseases ( Fig. 6) 25,49,50 . Further, Collard et al 9 and Wallwork et al 51 www.nature.com/scientificreports www.nature.com/scientificreports/ wheat populations which supports our finding 9,51 .…”

Section: Discussionmentioning

confidence: 99%

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat

Pariyar

Erginbaş-Orakci²,

Dadshani³

et al. 2020

Sci Rep

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Fusarium crown rot (FCR) is one of the most important diseases of wheat (Triticum aestivum L.). fcRis mainly caused by the fungal pathogens Fusarium culmorum and F. pseudograminearum. In order to identify new sources of resistance to FCR and to dissect the complexity of FCR resistance, a panel of 161 wheat accessions was phenotyped under growth room (GR) and greenhouse conditions (GH). Analysis of variance showed significant differences in crown rot development among wheat accessions and high heritability of genotype-environment interactions for GR (0.96) and GH (0.91). Mixed linear model analysis revealed seven novel quantitative trait loci (QTLs) linked to F. culmorum on chromosomes 2AL, 3AS, 4BS, 5BS, 5DS, 5DL and 6DS for GR and eight QTLs on chromosomes on 3AS, 3BS, 3DL, 4BS (2), 5BS, 6BS and 6BL for GH. Total phenotypic variances (R²) explained by the QTLs linked to GR and GH were 48% and 59%, respectively. In addition, five favorable epistasis interactions among the QTLs were detected for both GR and GH with and without main effects. Epistatic interaction contributed additional variation up to 21% under GR and 7% under GH indicating strong effects of environment on the expression of QTLs. Our results revealed FCR resistance responses in wheat to be complex and controlled by multiple QTLs.www.nature.com/scientificreports www.nature.com/scientificreports/ of inheritance, and strong genotype-by-environment interaction 20-23 . Further, molecular mechanisms of FCR resistance and interaction with environment are poorly understood. Thus, characterizing potential epistatic interactions between QTLs and environment will provide new insight in understanding the genetic basis of FCR resistance.Wheat accessions of different origin comprise a broad genetic diversity which can be exploited to breed for high yielding and disease resistant cultivars. Current progress in high density mapping, sequence availability and statistical tools make it possible to uncover the majority of QTL effects and functionally characterize their role in host-pathogen interactions. Genome-wide association study (GWAS) is a powerful approach to detect associations between phenotypic variation and genetic polymorphisms; in this way, QTLs for traits such as FCR can be located in the genome. GWAS utilizes linkage disequilibrium to dissect the genetic architecture of complex traits by correlating phenotypes to genotypes. It has been used earlier to identify QTLs that are involved in plant responses to soil borne pathogens in a large set of unrelated wheat accessions 19,24 . Detection of QTLs tightly linked to gene/s that control FCR resistance are the pre-requisite for marker assisted breeding. In the present study, we analyze the genetic basis of the FCR infection, disease development, and the interaction accross multiple environments. Our study not only confirmed previously described QTLs linked to FCR but also identified novel QTLs that have additive main and epistatic effects on FCR resistance which upon functional validation would provide addit...

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“…Fhb1 expresses a dominant phenotype (Xie et al 2007 and own unpublished data). Consequently, possible explanations for the phenotypic difference could be induced expression of the underlying gene in response to the pathogen or constitutive expression in the resistant genotype, or absence of the respective gene in susceptible lines as Chinese Spring and Remus.…”

Section: Discussionmentioning

confidence: 99%

Suppressed recombination and unique candidate genes in the divergent haplotype encoding Fhb1, a major Fusarium head blight resistance locus in wheat

Schweiger

Steiner

Vautrin³

et al. 2016

Theor Appl Genet

100

103

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Key messageFine mapping and sequencing revealed 28 genes in the non-recombining haplotype containingFhb1. Of these, only a GDSL lipase gene shows a pathogen-dependent expression pattern.AbstractFhb1 is a prominent Fusarium head blight resistance locus of wheat, which has been successfully introgressed in adapted breeding material, where it confers a significant increase in overall resistance to the causal pathogen Fusarium graminearum and the fungal virulence factor and mycotoxin deoxynivalenol. The Fhb1 region has been resolved for the susceptible wheat reference genotype Chinese Spring, yet the causal gene itself has not been identified in resistant cultivars. Here, we report the establishment of a 1 Mb contig embracing Fhb1 in the donor line CM-82036. Sequencing revealed that the region of Fhb1 deviates from the Chinese Spring reference in DNA size and gene content, which explains the repressed recombination at the locus in the performed fine mapping. Differences in genes expression between near-isogenic lines segregating for Fhb1 challenged with F. graminearum or treated with mock were investigated in a time-course experiment by RNA sequencing. Several candidate genes were identified, including a pathogen-responsive GDSL lipase absent in susceptible lines. The sequence of the Fhb1 region, the resulting list of candidate genes, and near-diagnostic KASP markers for Fhb1 constitute a valuable resource for breeding and further studies aiming to identify the gene(s) responsible for F. graminearum and deoxynivalenol resistance.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-016-2727-x) contains supplementary material, which is available to authorized users.

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The effect of 3BS locus of Sumai 3 on Fusarium head blight resistance in Australian wheats

Cited by 20 publications

References 23 publications

Microsatellite marker-assisted selection of fusarium head blight resistance in backcrossed Japanese winter wheat lines

Microsatellite marker-assisted selection of fusarium head blight resistance in backcrossed Japanese winter wheat lines

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat

Suppressed recombination and unique candidate genes in the divergent haplotype encoding Fhb1, a major Fusarium head blight resistance locus in wheat

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