The effect of 3,5,3′-triiodothyronine supplementation on zebrafish (Danio rerio) embryonic development and expression of iodothyronine deiodinases and thyroid hormone receptors

Walpita, Chaminda; Geyten, Serge Van der; Rurangwa, E.; Darras, Veerle

doi:10.1016/j.ygcen.2007.02.020

Cited by 127 publications

(92 citation statements)

References 45 publications

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“…However, previous analysis of THs during embryo development in fathead minnow also showed a significant rise in both T4 and T3 during the prehatch period, indicating embryonic production of both thyroid hormones and significantly increased synthesis of THs in an early developmental stage after hatching in fish. 24,25 In the present study, TH levels were increased in the larvae and the expressions of genes related to thyroid development and growth (e.g., hhex, nkx2.1) and TH synthesis (e.g., TG) were all significantly up-regulated as well, indicating that the increased THs in the larvae could also be …”

Section: ' Discussionsupporting

confidence: 54%

“…Treatment with 5 nM T3 was shown to downregulate Dio2 mRNA expression in zebrafish larvae, without affecting Dio1 expression. 25 Knockdown of Dio1 alone does not affect developmental progression, while Dio2 knockdown results in a clear developmental delay, 32 suggesting that Dio2 is the major contributor to TH activation in developing zebrafish embryos/larvae. These results indicate that zebrafish larvae are dependent on T4 to T3 conversion for normal development, and down-regulation of Dio2 transcription may affect zebrafish development.…”

Section: Environmental Science and Technologymentioning

confidence: 99%

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Parental Transfer of Polybrominated Diphenyl Ethers (PBDEs) and Thyroid Endocrine Disruption in Zebrafish

Lam

Guo

et al. 2011

Environ. Sci. Technol.

189

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Polybrominated diphenyl ethers (PBDEs) have the potential to disrupt the thyroid endocrine system. The objective of the present study was to characterize the disrupting effects of longterm exposure on the thyroid endocrine system in adult fish and their progeny following parental exposure to PBDEs. Zebrafish (Danio rerio) embryos were exposed to environmentally relevant concentrations (1, 3, and 10 μg/L) of the PBDE mixture DE-71 for 5 months until sexual maturation. In the F0 generation, exposure to DE-71 significantly increased plasma thyroxine (T4) but not 3,5,3 0 -triiodothyronine (T3) in females. This increased T4 was accompanied by decreased mRNA levels of corticotropinreleasing hormone (CRH) and thyrotropin β-subunit (TSHβ) in the brain. The F1 generation was further examined with or without continued DE-71 treatment conditions. Exposure to DE-71 in the F0 fish caused significant increases in T4 and T3 levels in the F1 larvae and modified gene expressions in the hypothalamicÀ pituitaryÀthyroid axis (HPT axis) under both conditions. Decreased hatching and inhibition of growth in the F1 offspring were observed in the condition without DE-71 treatment. Continued DE-71 treatment in the F1 embryos/larvae resulted in further decreased hatching, and increased malformation rates compared with those without DE-71 exposure. Analysis of F1 eggs indicated that parental exposure to DE-71 could result in a transfer of PBDEs and thyroid hormones (THs) to their offspring. For the first time, we demonstrated that parental exposure to low concentrations of PBDEs could affect THs in the offspring and the transgenerational PBDE-induced toxicity in subsequent nonexposed generations.

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Section: ' Discussionsupporting

confidence: 54%

Section: Environmental Science and Technologymentioning

confidence: 99%

Parental Transfer of Polybrominated Diphenyl Ethers (PBDEs) and Thyroid Endocrine Disruption in Zebrafish

Lam

Guo

et al. 2011

Environ. Sci. Technol.

189

View full text Add to dashboard Cite

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“…A recent study showed that thyroid hormones are present in high quantities in zebrafish eggs and are presumably of maternal origin during embryogenesis. Concentrations of thyroxine (T4) and triiodothyronine (T3) in the embryos showed no significant change during early development (8 hpf-75 hpf) and decreased until endogenous production began (Walpita et al, 2007). Possible explanations for the elevation of hhex and pax8 expressions may be related to the promotion of the growth of the thyroid primordium to compensate for changes in egg TH levels.…”

Section: Discussionmentioning

confidence: 99%

Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS

Shi

Lam

et al. 2008

Toxicology and Applied Pharmacology

322

160

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“…In the context of previous studies in teleosts, the autoinduction of tra and trb mRNAs by T 3 appears consistent across developmental stages after embryogenesis. In zebrafish embryos, tra mRNAs appear to be T 3 regulated while trb is not (Walpita et al 2007), but in larvae of this species transcripts for tra1 and trb1 have both been shown to be upregulated in vivo in a dose-dependent manner by T 3 (Liu et al 2000). In Senegalese sole (Solea senegalensis), mRNAs for trb are elevated during larval metamorphosis, and experimental manipulations revealed that trb transcript levels increase in response to exogenous T 4 -and decrease in response to thiouracil -during the metamorphic transition in this flatfish (Manchado et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Thyroid hormone regulation of mRNAs encoding thyrotropin β-subunit, glycoprotein α-subunit, and thyroid hormone receptors α and β in brain, pituitary gland, liver, and gonads of an adult teleost, Pimephales promelas

Lema¹,

Dickey²,

Schultz³

et al. 2009

Journal of Endocrinology

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Thyroid hormone regulation of mRNAs encoding thyrotropin b-subunit, glycoprotein a-subunit, and thyroid hormone receptors a and b in brain, pituitary gland, liver, and gonads of an adult teleost, Pimephales promelas AbstractThyroid hormones (THs) regulate growth, morphological development, and migratory behaviors in teleost fish, yet little is known about the transcriptional dynamics of gene targets for THs in these taxa. Here, we characterized TH regulation of mRNAs encoding thyrotropin subunits and thyroid hormone receptors (TRs) in an adult teleost fish model, the fathead minnow (Pimephales promelas). Breeding pairs of adult minnows were fed diets containing 3,5, 3 0 -triiodo-L-thyronine (T 3 ) or the goitrogen methimazole for 10 days. In males and females, dietary intake of exogenous T 3 elevated circulating total T 3 , while methimazole depressed plasma levels of total thyroxine (T 4 ). In both sexes, this methimazole-induced reduction in T 4 led to elevated mRNA abundance for thyrotropin b-subunit (tshb) in the pituitary gland. Fish treated with T 3 had elevated transcript levels for TR isoforms a and b (tra and trb) in the liver and brain, but reduced levels of brain mRNA for the immediate-early gene basic transcription factor-binding protein (bteb). In the ovary and testis, exogenous T 3 elevated gene transcripts for tshb, glycoprotein hormone a-subunit ( gpha), and trb, while not affecting tra levels. Taken together, these results demonstrate negative feedback of T 4 on pituitary tshb, identify tra and trb as T 3 -autoinduced genes in the brain and liver, and provide new evidence that tshb, gpha, and trb are THs regulated in the gonad of teleosts. Adult teleost models are increasingly used to evaluate the endocrine-disrupting effects of chemical contaminants, and our results provide a systemic assessment of TH-responsive genes during that life stage.

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The effect of 3,5,3′-triiodothyronine supplementation on zebrafish (Danio rerio) embryonic development and expression of iodothyronine deiodinases and thyroid hormone receptors

Cited by 127 publications

References 45 publications

Parental Transfer of Polybrominated Diphenyl Ethers (PBDEs) and Thyroid Endocrine Disruption in Zebrafish

Parental Transfer of Polybrominated Diphenyl Ethers (PBDEs) and Thyroid Endocrine Disruption in Zebrafish

Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS

Thyroid hormone regulation of mRNAs encoding thyrotropin β-subunit, glycoprotein α-subunit, and thyroid hormone receptors α and β in brain, pituitary gland, liver, and gonads of an adult teleost, Pimephales promelas

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