The effect of 24-epibrassinolide on the green alga Acutodesmus obliquus (Chlorophyceae)

Talarek-Karwel, Marta; Bajguz, Andrzej; Piotrowska‐Niczyporuk, Alicja; Rajewska, Iwona

doi:10.1016/j.plaphy.2018.01.016

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…One of a highly active representative of BRs is 24epibrassinolide (EBL), which is the most often subjected to plants in experiments as a growth stimulator. EBL increases the cells number and the content of pigments (chlorophylls, carotenes, and xanthophylls), monosaccharide, and protein in C. vulgaris and A. obliquus (Bajguz and Czerpak 1998;Bajguz 2000b;Talarek-Karwel et al 2018). Moreover, previous studies confirmed the toxic effect of Pb ions on the unicellular green alga.…”

Section: Introductionsupporting

confidence: 53%

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24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

Talarek-Karwel

Bajguz

Piotrowska‐Niczyporuk

2019

J Appl Phycol

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Aquatic organisms are exposed to many stressors, e.g., heavy metals. Brassinosteroids, a plant hormone group, can effectively stimulate plants to defend against the negative impact of a heavy metal. The present study was conducted with an aim to find out the influence of 24-epibrassinolide (EBL) on Acutodesmus obliquus treated with 0.01 and 500 μM of lead (Pb) ions during 7 days of cultivation. Pb has a toxic effect on algal cultures because it limits both the growth and development and induces oxidative stress. Simultaneously, 1 μM EBL was involved in protecting algal cells against the toxic effect of Pb. Despite the presence of Pb, EBL significantly increased the number of algal cells and their metabolite content (e.g., proteins, monosaccharides, chlorophylls, carotenes, and xanthophylls). Cultures treated concurrently with EBL and Pb were characterized by a reduction in their content of endogenous Pb, H 2 O 2 , and malondialdehyde. Also, EBL increased the activity of catalase, ascorbate peroxidase, superoxide dismutase, glutathione reductase, and the content of ascorbate and glutathione. EBL increased the phytochelatin synthase activity, thereby enhancing the production of phytochelatins accountable for both binding and detoxification of Pb. These results indicate the influence of EBL on the inhibitory effect of Pb in A. obliquus. These findings help to clarify the role of BRs in the algal adaptation to the prevailing stressful conditions.

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Section: Introductionsupporting

confidence: 53%

“…Previous research confirmed that the most stimulating impact of EBL (Sigma-Aldrich, USA) occurred at a concentration of 1 μM after the application of six different EBL concentrations from 0.0001 to 10 μM (with a multiplier of 10) on A. obliquus (Talarek-Karwel et al 2018). Hence, 1 μM EBL was chosen in this experiment.…”

Section: Algal Cultivationmentioning

confidence: 89%

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

Talarek-Karwel

Bajguz

Piotrowska‐Niczyporuk

2019

J Appl Phycol

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“…The direct function of BRs in photosynthesis is the increase of plants’ light-capturing efficiency and induction of the activity of chlorophyll biosynthesis enzymes [ 8 ]. Referring to the previous studies, EBL caused the increase of pigment content in alga A. obliquus [ 42 ], C. vulgaris [ 57 ], and wheat [ 58 ]. In another study, Maity and Bera [ 59 ] observed the promotive effect of BL on the total chlorophylls’ content in the mung bean ( Vigna radiata ).…”

Section: Discussionmentioning

confidence: 87%

“…Other studies demonstrated the stimulatory effect of BRs; however, the growth rate under the influence of various BR concentrations differed among plant species. For instance, in the green alga Acutodesmus obliquus , the most promotive effect on the number of algal cells was reached at a concentration of 1 µM EBL [ 42 ], while the greatest increases of the Chlorella vulgaris growth was observed in alga treated with 0.01 µM BL [ 43 ]. In another study, the most promotive effect on the weight of Brassica junceae shoots and roots was noted after treatment with 0.5 µM BL [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…Both chlorophylls and carotenoids are essential to the light phase of photosynthesis [ 52 ]. The presence of photosynthetic pigments with the specific individual xanthophyll was confirmed mainly in algae [ 42 , 53 ] and useless agricultural plants, e.g., maize ( Zea mays ) [ 54 ], wheat [ 55 ], or soybean ( Glycine max ) [ 56 ]. While in the present studies, the occurrence of two chlorophylls and eight carotenoids was also confirmed ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

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Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Chmur

Bajguz

2021

Plants

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Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.

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Exploration of the phytohormone regulation of energy storage compound accumulation in microalgae

Saud

Jiang

et al. 2022

Food and Energy Security

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Microalgal energy storage compounds (carbohydrates, lipids, etc.) can serve as renewable feedstocks for biofuels and biobased chemicals. Traditional methods of inducing the accumulation of energy storage compounds in microalgae, such as abiotic stress (high light intensity, high salinity, nutrient limitation, heavy metals, etc.), can affect the growth of microalgae and limit their efficient accumulation of energy storage materials. Plant hormones are a class of small molecular substances that act as chemical messengers to coordinate plant cell activities and regulate the physiological and metabolic activities of microalgae, including promoting microalgal cell proliferation, improving stress resistance, and enhancing photosynthetic activity, thereby increasing algal biomass and lipid, chlorophyll and protein content. This paper reviews the recent research progress on regulation of the accumulation of energy storage compounds in microalgae by adding exogenous plant hormones combined with abiotic stress, discusses the mechanism of plant hormones regarding the accumulation of energy storage compounds in microalgae, and proposes future research needs.

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The effect of 24-epibrassinolide on the green alga Acutodesmus obliquus (Chlorophyceae)

Cited by 17 publications

References 35 publications

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

24-Epibrassinolide modulates primary metabolites, antioxidants, and phytochelatins in Acutodesmus obliquus exposed to lead stress

Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Exploration of the phytohormone regulation of energy storage compound accumulation in microalgae

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