The effect of 2,4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana

The role of defense signaling pathways in the interaction of Arabidopsis thaliana and Vertcillium longisporum

“…The difference between wild-type and coi1-t vascular alterations at 15 dpi might be the transition state of these cells which seems to be delayed in coi1-t. To investigate this further, V. longisporum-induced alteration in vascular bundles were studied in wild-type and coi1-t plants at 10 dpi and was found that these characteristically stained cells were also observed in the wild-type at this early stage of the disease. The appearance of cells with dense cytosol in the vascular bundle has been described before when petioles were treated with 1 mg/L 2,4-dichlorophenoxyacetic (Li et al, 2012). As previously observed for V. albo-atruminfected hop (Talboys, 1958), Arabidopsis forms additional xylem-like cells.…”

Involvement of Plant Stem Cells or Stem Cell-Like Cells in Dedifferentiation

“…Our previous studies showed that stem cells initially remain in the vascular bundle and divide, and callus is formed after petiole explants are cultured for 24–36 h ( Yu et al, 2010 ; Li et al, 2011a ). The mRNA chips were analyzed to screen related genes.…”

“…However, the direct relationship of WINDs to stem cell niche remains inconclusive. WIND activates cytokinin signaling but not auxin signaling, whereas auxin alone, not cytokinin alone, can induce callus formation ( Li et al, 2011a ). As such, dedifferentiation may involve several pathways comprising stem cell-related genes.…”