The effect of 17% EDTA and MTAD on smear layer removal and on erosion of root canal dentin when used as final rinse: An in vitro SEM study

Mahajan, Vishal A.; Kamra, Abhay; Dahiwale, Sonali

doi:10.4103/2231-0754.95282

Cited by 7 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MTAD (a mixture of tetracycline, acid and a detergent) has been the focus of attention as an alternative root canal irrigant. Use of MTAD has been reported to be more efficient in removing smear layer as compared with the use of EDTA and NaOCl, especially from the apical third[ 4 ] without significantly changing the structure of the dentinal tubules. [ 5 ] It is also less cytotoxic and more biocompatible than other irrigant solutions.…”

Section: Introductionmentioning

confidence: 99%

Assessment of apical seal obtained after irrigation of root end cavity with MTAD followed by subsequent retrofilling with MTA and Biodentine: An in vitro study

et al. 2015

View full text Add to dashboard Cite

Introduction:The present study is designed to assess the apical seal obtained after root end cavity irrigation with MTAD and retrograde filling with mineral trioxide aggregate (MTA) and Biodentine.Materials and Methods:Sixty extracted maxillary central incisors were instrumented and obturated. Apical 3 mm of all the roots were resected and retrograde preparations of 3 mm were made in all the teeth using ultrasonic tips. Thirty root end preparations were irrigated with MTAD for 5 min (Group 1), while 30 were irrigated with saline for 5 min (Group 2). Each main group was subdivided into two subgroups containing 15 samples each. Samples under each subgroup were then filled either with MTA (1A and 2A) or Biodentine (1B and 2B). The amount of microleakage was assessed using a UV spectrophotometer.Results:Statistical analysis suggest a significant difference (P < 0.005) between group 1A and 2A and between the groups 1B and 2B (P < 0.1). There was a significant difference (P < 0.005) between group 1A and 1B and also between group 2A and 2B.Conclusion:Irrigation with MTAD significantly improved the apical seal of Biodentine, but it increased the microleakage in MTA-filled root end cavities. Also the apical seal obtained with Biodentine was superior to that obtained with MTA.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of apical seal obtained after irrigation of root end cavity with MTAD followed by subsequent retrofilling with MTA and Biodentine: An in vitro study

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The removal of the smear layer helps eliminate the microorganisms by allowing deeper penetration of irrigants and intracanal medicaments. It also helps achieve a good seal with the obturating materials by allowing deep penetration of the root canal sealers and by intimate adaptation of the obturating material [ 8 ]. Effective mechanical instrumentation followed by thorough chemical irrigation is necessary to achieve proper disinfection of the root canal.…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of canal cleanliness at apical third using Self-Adjusting File and Wave One File with different irrigants: an in vitro scanning electron microscopic study

Balakrishnan¹,

Ranjani

Amudhalakshmi

et al. 2023

Medicine and Pharmacy Reports

View full text Add to dashboard Cite

Aim. To evaluate the cleaning ability of two single-file rotary systems- Self-Adjusting File (SAF) and Wave one (WO) systems in removing the smear layer using sodium hypochlorite (NaOCl) + ethylene diamine tetraacetic acid (EDTA) and NaOCl + Qmix as irrigants at apical one-third of the root canal. Methods. Forty extracted human mandibular premolars were selected and working length was determined. The canal was manually instrumented up to a number 25 size K-file. The roots were divided into the following groups with 10 samples each – Group 1 using SAF: Group 1a- 3% NaOCl + 17% EDTA, Group 1b- 3% NaOCl + Qmix. Group2 using WO: Group 2a- 3% NaOCl + 17% EDTA, Group 2b- 3% NaOCl + Qmix. In the SAF group, the irrigation was performed continuously using the special irrigation apparatus. In the WO group, syringe irrigation was done followed by final irrigant activation using passive ultrasonic irrigation (PUI). The roots were sectioned longitudinally and subjected to scanning electron microscopic (SEM) examination. The amount of smear layer was evaluated using a five score index at the apical third level. Statistical analysis was performed using the Chi-square test. Results. Group 1 (SAF) showed better canal cleanliness at apical third compared to Group 2 (WO) with both irrigant combinations and the results were statistically significant (p<0.05). 3% NaOCl + Qmix was equally as effective as 3% NaOCl + 17% EDTA in removing the smear layer with no significant difference between them. Conclusion. Within the limitation of this study, SAF in combination with 3% NaOCl + Qmix or 3% NaOCl + 17% EDTA should be used for removing smear layer in critical areas of the root canal.

show abstract

“…Most of the root canal irrigants aim at the disruption of these bio lms and smear layer to achieve bacterial elimination at a suboptimal level to facilitate periapical healing. Mixture of 3% doxycycline, 4.25% citric acid, and Tween-80, a detergent (MTAD) is one of the irrigants which exhibits both smear layer removal ability and antibacterial action with least cytotoxicity [3].It is highly effective in removal of smear layer [4] and exert its anti-bacterial activity better than Chlorhexidine(CHX) and sodium hypochlorite(NaOCl) [5].…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of effect of nisin-incorporated ethylenediamine tetraacetic acid and MTAD on endodontic biofilm eradication, smear layer removal, and depth of sealer penetration

Rao,

Sandeep,

Madhubala

et al. 2023

Clin Oral Invest

View full text Add to dashboard Cite

ObjectivesTo comparatively evaluate the Nisin incorporated ethylenediamine tetraacetic acid (N-EDTA) and MTAD on cytotoxicity, endodontic bio lm eradication potential, smear layer removal ability and sealer penetration depth. Materials and methodsN-EDTA was prepared and characterized using High performance liquid chromatography(HPLC).Minimum Inhibitory, Minimum Bactericidal and Minimum Bio lm Inhibitory Concentration(MBC,MIC and MBIC) were determined. Cytocompatibility of N-EDTA and MTAD was evaluated using 3,(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) based colorimetric assay. Dentin specimens (n = 88 for antibacterial analysis, n=170 for sealer penetration depth) were prepared and subjected to the classical irrigating strategy and obturation respectively. The scanning electron microscopic evaluation(SEM) was done for evaluation of bio lm disruption and smear layer removal. Confocal laser scanning microscopy(CLSM) evaluation was done for determining percentage of bacterial viability and sealer penetration depth. Statistical analysis of One-way ANOVA and Tukey's HSD post hoc tests for bacterial viability, Kruskal-Wallis test and Mann Whitney test for smear layer removal and depth of penetration were done with the signi cance level set at p<0.05. ResultsMTAD and N-EDTA showed cytocompatibility without any statistical difference each other. For N-EDTA, the MIC and MBC values were 12.5μg/ml (1:8) and MBIC values were 36 μg/ml. Bio lm disruption and killed bacterial percentage of N-EDTA was statistically higher than MTAD, whereas both the materials showed similar e cacy in the removal of smear layer and sealer penetration depth. ConclusionN-EDTA had negligible cytotoxicity with similar smear layer removal ability, sealer penetration and better antibio lm potential than MTAD Clinical relevance N-EDTA can serve as a viable alternative endodontic irrigant.

show abstract

The effect of 17% EDTA and MTAD on smear layer removal and on erosion of root canal dentin when used as final rinse: An in vitro SEM study

Cited by 7 publications

References 15 publications

Assessment of apical seal obtained after irrigation of root end cavity with MTAD followed by subsequent retrofilling with MTA and Biodentine: An in vitro study

Assessment of apical seal obtained after irrigation of root end cavity with MTAD followed by subsequent retrofilling with MTA and Biodentine: An in vitro study

Comparative evaluation of canal cleanliness at apical third using Self-Adjusting File and Wave One File with different irrigants: an in vitro scanning electron microscopic study

Comparative evaluation of effect of nisin-incorporated ethylenediamine tetraacetic acid and MTAD on endodontic biofilm eradication, smear layer removal, and depth of sealer penetration

Contact Info

Product

Resources

About