The Effect Follicular Fluid Vitamin A, E, D and B6 on Embryo Morphokinetics and Pregnancy Rates in Patients Receiving Assisted Reproduction

Abstract

<p><strong>Objective:</strong> To evaluate the associations among levels of vitamins A, E, D, and B6 in follicular fluid embryo morphokinetics and quality, and clinical pregnancy rates.</p><p><strong>Study Design:</strong> A total of 58 patients with unexplained infertility admitted to the in vitro fertilisation (IVF) centre of Izmir Medical Park Hospital were included in this prospective clinical study. For each patient, vitamin levels were assayed using high-performance liquid chromatography. After intracytoplasmic sperm injection, for each oocyte, the relationships between each vitamin and subsequent embryo quality, embryo morphokinetics, and clinical pregnancy rates were investigated. Embryos were classified as grade A, B, C, or D according to morphokinetic parameters using t5-t2 and t5-t3 (cc3). </p><p><strong>Results:</strong> There was no significant correlation between embryo morphokinetic parameters (tpnf, t2, t3, t4, t5, t6, t7 and t8) and follicular fluid vitamin (A, B6, D and E) levels (p>0.05). There was a significant positive correlation between t5optimal and follicular fluid vitamin A levels (p<0.05). There was a significant positive correlation between cc2optimal and follicular fluid vitamin B6 levels (p<0.05). Levels of vitamins A and B6 were significantly higher in grade A and B embryos than in grade C and D embryos. There were no significant relationships between vitamins E or D and embryo quality or between any vitamin and clinical pregnancy rates.</p><p><strong>Conclusion:</strong> High levels of vitamins A and B6 in follicular fluid are significantly associated with high-quality embryos and optimal morphokinetics. However, none of the vitamins considered showed a significant relationship with clinical pregnancy rates.</p>

LC-MS Analysis Revealed the Significantly Different Metabolic Profiles in Spent Culture Media of Human Embryos with Distinct Morphology, Karyotype and Implantation Outcomes

“…Glucose-dependent nucleotide synthesis by the pentose phosphate pathway was found to control activation pathway for trophectoderm specific gene transcription during mammalian embryogenesis [ 66 ]. Amino acids turnover and high levels of vitamins A and B6 were previously reported to be associated with aneuploidies, embryo quality, and morphokinetics [ 61 , 67 ]. The enrichment of amino acid and vitamin B6 metabolism pathways, as well as lipid-associated pathways, were also observed ( Table 5 , Table 6 and Table 7 ) in culture media of embryos with successful or unsuccessful implantation.…”

The effect of slow-release vaginal dinoprostone on maternal and fetal oxidative stress in term pregnancies complicated by oligohydramnios: Prospective cohort study

“…OS has been examined in cases of fibroids, assisted reproduction, pregnancy loss, gestational diabetes, preeclampsia, IUGR, nuchal cord, and fetal death. [9][10][11][12][13][14][15] Thiols (RSH) are a class of organic compounds that can undergo oxidation reactions via oxidants and form disulfide bonds. Under conditions of OS, dynamic thiol/disulfide homeostasis is maintained, and it plays critical roles in antioxidant protection, detoxification, signal transduction, apoptosis, regulation of enzymatic activity, transcription factors, and cellular signaling mechanisms.…”

“…OS has been examined in cases of fibroids, assisted reproduction, pregnancy loss, gestational diabetes, preeclampsia, IUGR, nuchal cord, and fetal death. 9–15…”