The EF-hand Ca2+-binding protein super-family: A genome-wide analysis of gene expression patterns in the adult mouse brain

Girard, F; Venail, Julien; Schwaller, Beat; Celio, Marco R.

doi:10.1016/j.neuroscience.2015.02.018

Cited by 41 publications

(35 citation statements)

References 97 publications

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“…Although EF-hand CaBPs have been extensively studied over the past decades, analysis of select members of a >150-member protein superfamily inevitably fails to resolve the many essential cellular contributions of these proteins, often leading to a role as mere, but still very useful, "markers" of particular cell types (Girard et al, 2015;Schwaller, 2009). Significant caveats of our present understanding are due to the lack of "deorphanization" efforts to identify new CaBPs containing EF-hand, and their rigid classification into buffer and sensor categories.…”

Section: Candidate Ef-hand Calcium-binding Proteinsmentioning

confidence: 99%

Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord

et al. 2016

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Neuronal calcium-binding protein 1 and -2 (NECAB1/2) localize to multiple excitatory neuron populations in the mouse spinal cord. Here, we analyzed rat and human spinal cord, combining in situ hybridization and immunohistochemistry, complementing newly collated data on mouse spinal cord for direct comparisons. Necab1/2 mRNA transcripts showed complementary distribution in rodent's spinal cord. Multiple-labeling fluorescence histochemistry with neuronal phenotypic markers localized NECAB1 to a dense fiber plexus in the dorsal horn, to neurons mainly in superficial layers and to commissural interneurons in both rodent species. NECAB1-positive (+) motor neurons were only found in mice. NECAB1 distribution in the human spinal cord was similar with the addition of NECAB1-like immunoreactivity surrounding myelinated axons. NECAB2 was mainly present in excitatory synaptic boutons in the dorsal horn of all three species, and often in calbindin-D28k(+) neuronal somata. Rodent ependymal cells expressed calbindin-D28k. In humans, they instead were NECAB2(+) and/or calretinin(+). Our results reveal that the association of NECAB2 to excitatory neuronal circuits in the spinal cord is evolutionarily conserved across the mammalian species investigated so far. In contrast, NECAB1 expression is more heterogeneous. Thus, our study suggests that the phenotypic segregation of NECAB1 and -2 to respective excitatory and inhibitory spinal systems can underpin functional modalities in determining the fidelity of synaptic neurotransmission and neuronal responsiveness, and might bear translational relevance to humans.

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Section: Candidate Ef-hand Calcium-binding Proteinsmentioning

confidence: 99%

Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord

et al. 2016

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“…Using immunohistochemical method, calb2a immunoreactivity has been demonstrated experimentally in the CNS and peripheral nervous system (PNS) of adult zebrafish (Castro et al, 2006a,b; Levanti et al, 2008) but there are no reports on calb2b localization in zebrafish brain. In other vertebrates except fish, a few studies have demonstrated the Calb2a and Calb2b mRNA expression in different regions of the CNS and PNS by using in situ hybridization technique (Cicchetti et al, 1998; Altobelli and Cimini, 2007; Zhao et al, 2013; Foo et al, 2014; Girard et al, 2015). Therefore, in the paucity of knowledge, by studying mRNA expression, the present investigation was aimed to understand the role of Calb2a and Calb2b during early embryonic development of D. rerio .…”

Section: Introductionmentioning

confidence: 99%

Neuroanatomical demonstration of calbindin 2a‐ and calbindin 2b‐like calcium binding proteins in the early embryonic development of zebrafish: mRNA study

Bhoyar

Jadhao²,

Sivasubbu

et al. 2017

Intl J of Devlp Neuroscience

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Certain calcium binding proteins (CaBPs) are essential for metabolic processes but the role of these proteins in the development is not well known. We have investigated the mRNA expression of CaBPs, calbindin 2a (Calb2a) and calbindin 2b (Calb2b) in the zebrafish embryos 24, 36, 48 and 72h post fertilization (hpf). We have seen very high Calb2a mRNA expression in the tegmentum (Tg), midbrain-hindbrain boundary (Mhb), hindbrain (Hb), spinal cord (Sc), retina and cranial ganglion (Crg). Also very high Calb2b mRNA expression was noted in olfactory cells, cerebellum, Tg, Mhb, Hb, optic tectum, retina, retinal ganglion cell layer, retinal inner nuclear layer, Sc, Neural crest, infraorbital neuromasts, pharyngeal arch 3-7 skeleton and mandibular neuromasts. It is known that many factors are involved in the differentiation of Mhb. Here we are reporting for the first time the mRNA expression of CaBPs (Calb2a and Calb2b) in the Mhb indicating their role in the differentiation of Mhb and development of the brain, eyes and other tissues in the zebrafish. We suggest that Calb2a and Calb2b play an important role in the regulation of zebrafish early embryonic development.

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“…A recent survey of the Allen Brain Atlas examined gene expression profiles of the entire EF-hand superfamily (Girard et al, 2015). There is no expression of CHP2 and no data for CHP1, but tescalcin mRNA has been found to be present in many regions, including the main olfactory bulb, basal ganglia and amygdala.…”

Section: Subcellular Localization and Tissue Distribution Of Tescalcinmentioning

confidence: 99%

Emerging roles of the single EF-hand Ca2+ sensor tescalcin in the regulation of gene expression, cell growth and differentiation

Kolobynina

Solovyova

Levay

et al. 2016

Journal of Cell Science

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Tescalcin (TESC, also known as calcineurin-homologous protein 3, CHP3) is a 24-kDa EF-hand Ca2+-binding protein that has recently emerged as a regulator of cell differentiation and growth. The TESC gene has also been linked to human brain abnormalities, and high expression of tescalcin has been found in several cancers. The expression level of tescalcin changes dramatically during development and upon signal-induced cell differentiation. Recent studies have shown that tescalcin is not only subjected to up- or down-regulation, but also has an active role in pathways that drive cell growth and differentiation programs. At the molecular level, there is compelling experimental evidence showing that tescalcin can directly interact with and regulate the activities of the Na+/H+ exchanger NHE1, subunit 4 of the COP9 signalosome (CSN4) and protein kinase glycogen-synthase kinase 3 (GSK3). In hematopoetic precursor cells, tescalcin has been shown to couple activation of the extracellular signal-regulated kinase (ERK) cascade to the expression of transcription factors that control cell differentiation. The purpose of this Commentary is to summarize recent efforts that have served to characterize the biochemical, genetic and physiological attributes of tescalcin, and its unique role in the regulation of various cellular functions.

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The EF-hand Ca2+-binding protein super-family: A genome-wide analysis of gene expression patterns in the adult mouse brain

Cited by 41 publications

References 97 publications

Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord

Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord

Neuroanatomical demonstration of calbindin 2a‐ and calbindin 2b‐like calcium binding proteins in the early embryonic development of zebrafish: mRNA study

Emerging roles of the single EF-hand Ca2+ sensor tescalcin in the regulation of gene expression, cell growth and differentiation

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