The EcoChip 2: An Autonomous Sensor Platform for Multimodal Bio-environmental Monitoring of the Northern Habitat

Das, P. Sarati; Gagnon-Turcotte, Gabriel; Ouazaa, K.; Bouzid, Karim; Hosseini, S. Nazila; Bharucha, Eric; Tremblay, Denise M.; Moineau, Sylvain; Messaddeq, Younès; Corbeil, Jacques; Gosselin, Benoît

doi:10.1109/embc44109.2020.9176335

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…The fungal highway column consists of a small tube in which one medium section is in contact with the soil, while the second one, which is physically separated from the first, can only be colonized by bacteria when they are transported into it via fungal in-growth. Other sophisticated methods for in situ cultivation are iChip [92] and EcoChip2 [93]. They enable parallel cultivation and isolation of up to now uncultivated microbial species, and can elucidate the dependency of some organisms to one another, due to in situ growth factors, that cannot be simulated under standard laboratory conditions.…”

Section: Reconstructing the Spatial Heterogeneity Of Soilmentioning

confidence: 99%

Methods for Studying Bacterial–Fungal Interactions in the Microenvironments of Soil

2021

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Due to their small size, microorganisms directly experience only a tiny portion of the environmental heterogeneity manifested in the soil. The microscale variations in soil properties constrain the distribution of fungi and bacteria, and the extent to which they can interact with each other, thereby directly influencing their behavior and ecological roles. Thus, to obtain a realistic understanding of bacterial–fungal interactions, the spatiotemporal complexity of their microenvironments must be accounted for. The objective of this review is to further raise awareness of this important aspect and to discuss an overview of possible methodologies, some of easier applicability than others, that can be implemented in the experimental design in this field of research. The experimental design can be rationalized in three different scales, namely reconstructing the physicochemical complexity of the soil matrix, identifying and locating fungi and bacteria to depict their physical interactions, and, lastly, analyzing their molecular environment to describe their activity. In the long term, only relevant experimental data at the cell-to-cell level can provide the base for any solid theory or model that may serve for accurate functional prediction at the ecosystem level. The way to this level of application is still long, but we should all start small.

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Section: Reconstructing the Spatial Heterogeneity Of Soilmentioning

confidence: 99%

Methods for Studying Bacterial–Fungal Interactions in the Microenvironments of Soil

2021

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“…Market-available impedance analyzers can be found in portable formats, but their prices are prohibitive for large scale deployment. As an alternative to these instruments, low-power low-cost integrated chips exist with impedance analyzer capabilities [30][31][32][33] . These chips can be used as all-in-one-package solutions for low-cost impedance analysis, but are not as versatile as benchtop instruments and their excitation frequency often proves insufficient for microorganism characterization.…”

Section: Introductionmentioning

confidence: 99%

Portable Impedance-Sensing Device for Microorganism Characterization in the Field

Bouzid

Greener

Carrara

et al. 2023

Preprint

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A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their applicability. This study proposes a portable microfluidic device based on impedance flow-cytometry and electrical impedance spectroscopy that can detect and quantify the size of microparticles larger than 45 μm, such as algae and microplastics. The system is low cost ($300), portable (5 cm × 5 cm), low-power (1.2 W), and easily fabricated utilizing a 3D-printer and industrial printed circuit board technology. The main novelty we demonstrate is the use of square wave excitation signal for impedance measurements with quadrature phase-sensitive detectors. A linked algorithm removes the errors associated to higher order harmonics. After validating the performance of the device for complex impedance models, we used it to detect and differentiate between polyethylene microbeads of sizes between 63 μm and 83 μm, and buccal cells between 45 μm and 70 μm. A precision of 3% is reported for the measured impedance and a minimum size of 45 μm is reported for the particle characterization.

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Portable impedance-sensing device for microorganism characterization in the field

Bouzid

Greener

Carrara

et al. 2023

Sci Rep

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A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their applicability. This study proposes a portable microfluidic device based on impedance flow-cytometry and electrical impedance spectroscopy that can detect and quantify the size of microparticles larger than 45 µm, such as algae and microplastics. The system is low cost ($300), portable (5 cm $$\times$$ × 5 cm), low-power (1.2 W), and easily fabricated utilizing a 3D-printer and industrial printed circuit board technology. The main novelty we demonstrate is the use of square wave excitation signal for impedance measurements with quadrature phase-sensitive detectors. A linked algorithm removes the errors associated to higher order harmonics. After validating the performance of the device for complex impedance models, we used it to detect and differentiate between polyethylene microbeads of sizes between 63 and 83 µm, and buccal cells between 45 and 70 µm. A precision of 3% is reported for the measured impedance and a minimum size of 45 µm is reported for the particle characterization.

show abstract

The EcoChip 2: An Autonomous Sensor Platform for Multimodal Bio-environmental Monitoring of the Northern Habitat

Cited by 6 publications

References 5 publications

Methods for Studying Bacterial–Fungal Interactions in the Microenvironments of Soil

Methods for Studying Bacterial–Fungal Interactions in the Microenvironments of Soil

Portable Impedance-Sensing Device for Microorganism Characterization in the Field

Portable impedance-sensing device for microorganism characterization in the field

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