For a generation or more, the mass spectrometry that
developed
at the frontier of molecular biology was worlds apart from isotope
ratio mass spectrometry, a label-free approach done on optimized gas-source
magnetic sector instruments. Recent studies show that electrospray-ionization
Orbitraps and other mass spectrometers widely used in the life sciences
can be fine-tuned for high-precision isotope ratio analysis. Since
isotope patterns form everywhere in nature based on well-understood
principles, intramolecular isotope measurements allow unique insights
into a fascinating range of research topics. This Perspective introduces
a wider readership to current topics in stable isotope research with
the aim of discussing how soft-ionization mass spectrometry coupled
with ultrahigh mass resolution can enable long-envisioned progress.
We highlight novel prospects of observing isotopes in intact polar
compounds and speculate on future directions of this adventure into
the overlapping realms of biology, chemistry, and geology.