The Early Terrestrial Fungal Lineage of Conidiobolus—Transition from Saprotroph to Parasitic Lifestyle

Gryganskyi, Andrii P.; Nie, Yong; Hajek, Ann E.; Hodge, Kathie T.; Liu, Xiaoyong; Aadland, Kelsey; Voigt, Kerstin; Anishchenko, Iryna M.; Кутовенко, В Б; Kava, Liudmyla; Vuek, Antonina; Vilgalys, Rytas; Huang, Bo; Stajich, Jason E.

doi:10.3390/jof8080789

Cited by 14 publications

(22 citation statements)

References 21 publications

(25 reference statements)

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“…Our phylogenetic analyses of the 18S-28S rDNA and genome-scale datasets showed A. closterii actually belongs to Entomophthoromycotina despite its great ecological distinctiveness. Our phylogenetic analyses also strongly supported the recent taxonomic revisions of Ancylistaceae (Nie et al 2020;Gryganskyi et al 2022). Ancylistes closterii was sister to the clade of Entomophthoraceae + paraphyletic Conidiobolus s.l.…”

Section: Position Of a Closterii And Phylogeny Of Entomophthoromycotinasupporting

confidence: 83%

“…S3). However, two previous analyses showed different positions, sister to Entomophthoraceae (Gryganskyi et al 2022) or sister to Entomophthoraceae + Capillidiaceae + Neoconidiobolaceae (Möckel et al 2022). These incongruences were probably caused by abnormally divergent rDNA sequences of Batkoaceae.…”

Section: Discussionmentioning

confidence: 86%

“…The four major clades of Conidiobolus s.l. were recovered, which correspond to the families Batkoaceae, Capillidiaceae, Conidiobolaceae, and Neoconidiobolaceae (Gryganskyi et al 2022). The genus Microconidiobolus was not monophyletic in our analysis.…”

Section: Resultsmentioning

confidence: 99%

“…were rejected in the AU test (Table S3). Another lineage, Batkoaceae including the genus Batkoa and some Conidiobolus -like taxa (Gryganskyi et al 2022), was positioned outside of A. closterii in our 18S-28S rDNA phylogeny (Fig. S3).…”

Section: Discussionmentioning

confidence: 98%

“…was separated into five genera, Azygosporus, Capillidium, Neoconidiobolus, Microconidiobolus, and Conidiobolus sensu stricto (s.s.) (Nie et al 2020;Cai et al 2021). These genera were removed from Ancylistaceae and accommodated in newly established families, Capillidiaceae (Capillidium), Conidiobolaceae (Azygosporus, Conidiobolus, and Microconidiobolus), Neoconidiobolaceae (Neoconidiobolus) although the phylogeny of Ancylistes has never been examined (Gryganskyi et al 2022). DNA sequencing of Ancylistes is pivotal to justify the recent taxonomic revisions of Ancylistaceae.…”

Section: Introductionmentioning

confidence: 99%

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Genomic analysis ofAncylistes closterii, an enigmatic alga parasitic fungus in the arthropod-associated Entomophthoromycotina

Seto,

James

2023

Preprint

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Recent advances in fungal genome sequencing have dramatically altered our understanding of the phylogeny and evolution of Fungi. However, there are still many poorly studied obligate parasitic or symbiotic fungi for which we lack any genomic information or knowledge of where they fit in the fungal phylogeny.Ancylistes,an endoparasite of desmid green algae, is such an understudied fungal genus. This genus has been taxonomically placed in the group of arthropod pathogens and saprobes, Entomophthoromycotina in Zoopagomycota. Understanding the phylogenetic position ofAncylistesprovides insights into the nutritional evolution of Zoopagomycota, which is primarily composed of animal-associated fungi. In this study, we found and cultivatedAncylistes closteriiwith its hostClosteriumsp. and sequenced its genome to investigate its phylogenetic position and evolution. Phylogenetic analyses using rDNA and genome-scale datasets showed thatA. closteriiwas sister to other Entomophthoromycotina fungi, confirming the taxonomic position ofAncylistes. Despite the ecological distinctiveness betweenAncylistesand other Entomophthoromycotina fungi, our comparative genomic analyses revealed many shared traits of these fungi such as lineage-specific subtilases and hybrid histidine kinases.Ancylistesalso possessed unique genes among Zoopagomycota fungi, such as plant cell wall degrading enzymes which could be important for infection of algae.SignificanceImproved taxon sampling is important for inferring a robust phylogeny of Fungi. However, there are still poorly studied obligate parasitic taxa whose DNA sequencing is challenging, especially in Zoopagomycota, one of the early diverging lineages of Fungi. This study focused on a long-neglected algal parasite,Ancylistes closterii, which belongs to the arthropod-associated group, Entomophthoromycotina. We rediscoveredA. closteriiand established a dual culture of fungus and its host alga, which enabled the first molecular analysis of this enigmatic parasite. Our results provide new insights into the nutritional evolution of primarily animal-associated Zoopagomycota.

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Section: Position Of a Closterii And Phylogeny Of Entomophthoromycotinasupporting

confidence: 83%

Section: Discussionmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genomic analysis ofAncylistes closterii, an enigmatic alga parasitic fungus in the arthropod-associated Entomophthoromycotina

Seto,

James

2023

Preprint

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Diurnal patterns and conidial dynamics of Batkoa major, a generalist entomophthoralean pathogen

Hajek,

Harris

2023

Fungal Ecology

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Enumerating soil biodiversity

Anthony

Bender

Heijden

2023

Proc. Natl. Acad. Sci. U.S.A.

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Soil is an immense habitat for diverse organisms across the tree of life, but just how many organisms live in soil is surprisingly unknown. Previous efforts to enumerate soil biodiversity consider only certain types of organisms (e.g., animals) or report values for diverse groups without partitioning species that live in soil versus other habitats. Here, we reviewed the biodiversity literature to show that soil is likely home to 59 ± 15% of the species on Earth. We therefore estimate an approximately two times greater soil biodiversity than previous estimates, and we include representatives from the simplest (microbial) to most complex (mammals) organisms. Enchytraeidae have the greatest percentage of species in soil (98.6%), followed by fungi (90%), Plantae (85.5%), and Isoptera (84.2%). Our results demonstrate that soil is the most biodiverse singular habitat. By using this estimate of soil biodiversity, we can more accurately and quantitatively advocate for soil organismal conservation and restoration as a central goal of the Anthropocene.

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The Early Terrestrial Fungal Lineage of Conidiobolus—Transition from Saprotroph to Parasitic Lifestyle

Cited by 14 publications

References 21 publications

Genomic analysis ofAncylistes closterii, an enigmatic alga parasitic fungus in the arthropod-associated Entomophthoromycotina

Genomic analysis ofAncylistes closterii, an enigmatic alga parasitic fungus in the arthropod-associated Entomophthoromycotina

Diurnal patterns and conidial dynamics of Batkoa major, a generalist entomophthoralean pathogen

Enumerating soil biodiversity

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