The early nodulin gene SrEnod2 from Sesbania rostrata is inducible by cytokinin

Dehio, Christoph; Bruijn, Frans J. de

doi:10.1111/j.1365-313x.1992.00117.x

Cited by 27 publications

(44 citation statements)

References 68 publications

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“…In contrast to lb gene promoters, the promoter regions of the soybean N-23 and N-26 late nodulin genes did not contain complete OSE elements, although the nodulin consensus sequences 'AAAGAT' and 'CTCTT' were present [15,25]. Complete OSE elements were also absent in the promoter regions of the ENOD2 early nodulin genes from soybean [21] and S. rostrata [8] indicating the involvement of new regulatory elements in early nodulin gene expression. For S. rostrata, Dehio and de Bruijn [ 8 ] reported an induction of ENOD2 transcription by cytokinin.…”

Section: Introductionmentioning

confidence: 83%

“…Complete OSE elements were also absent in the promoter regions of the ENOD2 early nodulin genes from soybean [21] and S. rostrata [8] indicating the involvement of new regulatory elements in early nodulin gene expression. For S. rostrata, Dehio and de Bruijn [ 8 ] reported an induction of ENOD2 transcription by cytokinin. Lauridsen et al [21 ] found the specific expression of a gusA reporter gene controlled by the complete soybean ENOD2B promoter in the nodule parenchyma of transgenic nodules of Trifolium and Lotus.…”

Section: Introductionmentioning

confidence: 90%

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The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

et al. 1995

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We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/+10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/+10 promoter, whereas the -239/+10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/+10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of Vicia nodules.

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Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 90%

The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

et al. 1995

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“…We are interested to know whether this will prove to be the case with MsEnod12A and B and, toward this end, we are presently constructing transgenic Medicago plants containing the GUS gene fused to the putative promoter regions of both genes. This will allow us, in addition, to investigate how the spatial expression of these early nodulins is regulated by bacterial [10] and/or plant [2] factors.…”

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confidence: 99%

“…The early plant response to symbiotic bacteria is characterized by the transcriptional induction of several plant genes (the early nodulin or Enod genes) whose products are thought to play a role in the infection process and/or nodule development [12]. Although several Enod genes have been identified in pea [6,14,15,16], few such early plant response genes have been characterized in other legumes [see for example 2,3,4,16]. We are working on the Rhizobium meliloti -Medicago (alfalfa) symbiosis, a model system which is especially suited to the analysis of plant symbiotic gene expression since alfalfa, unlike pea, can be readily transformed and regenerated [ 1].…”

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confidence: 99%

Identification of two alfalfa early nodulin genes with homology to members of the pea Enod12 gene family

et al. 1993

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In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putative protein products of 8066 Da and 12849 Da, respectively, each with a signal sequence at the N-terminus followed by a repetitive proline-rich region. Based on their expression during the initial period of nodule development, MsEnod12A and B are alfalfa early nodulin genes.

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“…In addition, binding sites for nodule trans-acting factors were identified and analyzed [9,29]. The only early nodulin gene promoter sequences analyzed so far were the ENOD2 promoters from soybean [28] and S. rostrata [11].…”

Section: Introductionmentioning

confidence: 99%

The nodule-specific VfENOD-GRP3 gene encoding a glycine-rich early nodulin is located on chromosome I of Vicia faba L. and is predominantly expressed in the interzone II-III of root nodules

et al. 1995

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A nodule-specific cDNA was isolated from a Vicia faba L. nodule cDNA library. Since time course experiments revealed an early expression of this transcript in the nodule, this cDNA coded for an early nodulin and was designated VfENOD-GRP3. Based on tissue print hybridizations, we found a predominant expression of VfENOD-GRP3 transcripts in the interzone II-III region of broad bean root nodules. The encoded early nodulin ENOD-GRP3 was characterized by an N-terminal signal peptide and a C-terminal domain displaying a glycine content of 31%. Sequence analysis of a genomic VfENOD-GRP3 clone revealed that the signal peptide and the glycine-rich domain were specified by two separate exons. Primer extension experiments identified two adjacent transcription start sites for VfENOD-GRP3 transcripts. The common nodulin sequences 'AAAGAT' and 'CTCTT' were present five and three times on both DNA strands of the putative VfENOD-GRP3 promoter, respectively. Additionally, three sequence motifs resembling organ-specific elements of the soybean lbc3 gene promoter and a sequence similar to the binding site 1 for the nodule trans-acting factor Nat2 were identified. From Southern blot data and from sequence analysis of genomic PCR fragments, the presence of a VfENOD-GRP3 gene family was inferred. By PCR experiments using sequence-specific primers and DNA of microisolated chromosomes as a template, this family was located on the long arm of chromosome I.

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The early nodulin gene SrEnod2 from Sesbania rostrata is inducible by cytokinin

Cited by 27 publications

References 68 publications

The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

Identification of two alfalfa early nodulin genes with homology to members of the pea Enod12 gene family

The nodule-specific VfENOD-GRP3 gene encoding a glycine-rich early nodulin is located on chromosome I of Vicia faba L. and is predominantly expressed in the interzone II-III of root nodules

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