The early effects of partial outflow obstruction on contractile properties of diabetic and non-diabetic rat bladder

Öztürk, Bülent; Çetinkaya, Mesut; Gür, Serap; Uğurlu, Özgür; Öztekin, Volkan; Aki, Fazıl Tuncay

doi:10.1007/s00240-002-0247-4

Cited by 12 publications

(15 citation statements)

References 20 publications

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“…In the current study, we found that 8 weeks of diabetes caused increases in in vitro contractile responses to EFS, which is consistent with several previous studies [16,17] . EFS results in a release of several neurohumoral transmitters, which in turn stimulate postsynaptic receptors mediating contraction.…”

Section: Discussionsupporting

confidence: 82%

<i>L</i>-Carnitine Treatment Partially Restores Urinary Bladder Function of Streptozotocin Diabetic Rats

Gür¹,

Irat²

2008

Urol Int

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Introduction: Diabetes mellitus is associated with urinary bladder dysfunction. This study determined whether or not detrusor responses were altered and reversed by L-carnitine treatment in the urinary bladders of diabetic rats. Materials and Methods: Three groups of animals were used: streptozotocin-treated (45 mg·kg^–1 i.p., 8 weeks), parallel L-carnitine-treated (0.6 g·kg^–1·day^–1 i.p.), and control rats. Contractile and relaxant responses were measured using isolated bath techniques. Results: Serum glucose levels in diabetic rats were partially reversed after L-carnitine treatment. Detrusor strips from diabetic rats exhibited an increase in response to electrical field stimulation (EFS; 0.5–32 Hz). Treatment with L-carnitine restored the hyperreactivity to EFS- induced contractility. The response to direct activation of the smooth muscle with carbachol and KCl remained unaltered. In relaxation studies, the urinary bladders of diabetic rats displayed a diminished response to isoprenaline, an unchanged response to ATP, and an increased response to adenosine of the ATP metabolite. L-Carnitine treatment restored the hyporesponsiveness of isoprenaline and the hyperresponsiveness of adenosine-elicited relaxation. Conclusions: These findings show that carnitine can be implicated in the contractile response of noradrenergic noncholinergic nerve stimulation and the relaxation response of isoprenaline and adenosine. Hence, L-carnitine deficiency can contribute to voiding deficiency in diabetic patients.

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Section: Discussionsupporting

confidence: 82%

<i>L</i>-Carnitine Treatment Partially Restores Urinary Bladder Function of Streptozotocin Diabetic Rats

Gür¹,

Irat²

2008

Urol Int

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“…Other models of markedly enhanced diuresis such as sucrose feeding and Brattleboro rats often but not always mimicked findings with STZ injection. BOO as diuresis‐independent model of bladder hypertrophy always caused different changes of muscarinic responses as compared to STZ injection and the combination of both pathologies exhibited a complex response . A study comparing two diuresis‐independent models of bladder hypertrophy, that is, surgically‐induced denervation and BOO also found discordant alterations of muscarinic responses, giving further support to the hypothesis that changes in contractility are related to a specific model and not directly to the presence of bladder hypertrophy.…”

Section: Exploration Of Links Between Hypertrophy and Bladder Dysfuncmentioning

confidence: 68%

“…While one study from the Longhurst group reported a reduced EFS response in bladder base and dome, later studies from this group and others reported increased EFS responses in STZ‐treated rats. Other reports from the Longhurst group and others found no major change in EFS‐induced contraction.…”

Section: Exploration Of Links Between Hypertrophy and Bladder Dysfuncmentioning

confidence: 96%

“…Two studies from different labs compared two states of bladder hypertrophy, either by STZ injection or by BOO induction. One of them found increased EFS responses after STZ administration, decreased responses after BOO and initially decreased but then recovering responses after a combination of STZ injection and BOO induction . Others found a lack of change of EFS response after STZ administration, a reduction after BOO and an even greater reduction with the combination of STZ and BOO .…”

Section: Exploration Of Links Between Hypertrophy and Bladder Dysfuncmentioning

confidence: 99%

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A systematic review of urinary bladder hypertrophy in experimental diabetes: Part 2. Comparison of animal models and functional consequences

Ellenbroek

İnan

Michel

2018

Neurourology and Urodynamics

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“…It has been suggested in recent studies that the purinergic system also contributes to bladder contractility, and it can become dominant under pathological conditions. In addition, neuronal and myogenic components consisting the detrusor contractility under pathological conditions have also become the topics of various studies [9,10] .…”

Section: Discussionmentioning

confidence: 99%

Alteration in Contractile Responses in Human Detrusor Smooth Muscle from Obstructed Bladders with Overactivity

et al. 2008

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Introduction: In this study, we aimed to evaluate changes in contractile responses under in vitro conditions in detrusor overactivity (DO) in patients with bladder outflow obstruction (BOO). Materials and Methods: Detrusor strips obtained during open prostatectomy procedure from 16 patients with BOO related to benign prostate hyperplasia were evaluated under in vitro conditions. Patients were assigned to two groups as patients with (DO) and without (no DO) DO. Four detrusor strips were prepared from each bladder in dimensions of 2 × 10 mm, and were suspended in organ bath. Responses to carbachol (10^–8 to 10^–3M), electrical field stimulation (EFS) (0.5–32 Hz), single-dose adenosine 5′-triphosphate (ATP) (10^–3M) and KCl (120 mM) were recorded to evaluate the contractile responses. EFS responses were repeated in the presence of NG-nitro-L-arginine methyl ester (L-NAME; 10 µM) and L-NAME + indomethacin. All responses were expressed as mg tension developed per mg of bladder tissue. Data obtained were compared using independent t test and one-way ANOVA test. Values of p < 0.05 were accepted as statistically significant. Results: Of the 16 patients on whom open prostatectomy was performed because of BOO, 8 of the patients were determined as no DO and 8 as DO. There were no differences between groups regarding age and residual urine. We found statistically significant differences between groups regarding dimensions of prostate, maximum bladder capacity and maximum bladder pressure. In the comparison of cumulative dose of carbachol, it was seen that responses were higher in the DO group, but the differences were not statistically significant. In EFS application, contractile responses were found to increase significantly in the DO group. No changes were observed between groups for ATP and KCl. EFS responses were found to be significantly higher in presence of L-NAME + indomethacin in the no DO group; however, no difference was seen in the DO group. Conclusions: Detrusor contractile responses to EFS increased in patients with BOO in presence of overactivity. These changes in contractile responses are observed possibly as a result of deterioration in neuromodulation, rather than as a result of changes in purinergic or cholinergic receptor sensation or level. We suggest that a noncholinergic-nonpurinergic mechanism can have some effect on these changes.

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The early effects of partial outflow obstruction on contractile properties of diabetic and non-diabetic rat bladder

Cited by 12 publications

References 20 publications

<i>L</i>-Carnitine Treatment Partially Restores Urinary Bladder Function of Streptozotocin Diabetic Rats

<i>L</i>-Carnitine Treatment Partially Restores Urinary Bladder Function of Streptozotocin Diabetic Rats

A systematic review of urinary bladder hypertrophy in experimental diabetes: Part 2. Comparison of animal models and functional consequences

Alteration in Contractile Responses in Human Detrusor Smooth Muscle from Obstructed Bladders with Overactivity

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