The Early Delayed Effect of Accelerated Carbon Ions and Protons on the Cognitive Functions of Mice

Сорокина, С. С.; Заичкина, С. И.; Rozanova, O. M.; Shemyakov, A. E.; Smirnova, E. H.; Dyukina, A. R.; Malkov, Anton; Балакин, В.Е.; Pikalov, V. A.

doi:10.1134/s1062359020120109

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…A few studies have established that single particle type exposures lead to fine motor deficits in rats (Blackwell et al . 2021), but differences in gross locomotion are scarce in the literature with some studies reporting that post-IRR OF locomotion is increased (Sorokina et al . 2020; Kokhan et al .…”

Section: Discussionmentioning

confidence: 99%

“…10). A few studies have established that single particle type exposures lead to fine motor deficits in rats (Blackwell et al 2021), but differences in gross locomotion are scarce in the literature with some studies reporting that post-IRR OF locomotion is increased (Sorokina et al 2020;Kokhan et al 2019;Liu et al 2019;Boutros et al 2021), decreased (Allen et al 2015;Holshouser et al 2020), or unchanged (Impey et al 2023). Time post-IRR and dose may be important in seeing 5-ion (1H, 2 x 1,000 MeV/n; 4He, 400 MeV/n; 16O, 400 MeV/n; 28Si, 600 MeV/n) GCR-induced changes in locomotion (Puukila et al 2023), although there are conflicting results (Boutros et al 2021).…”

Section: Discussionmentioning

confidence: 99%

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The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

Yun,

Kiffer,

Bancroft

et al. 2024

Preprint

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Galactic cosmic radiation (GCR) is an unavoidable risk to astronauts that may affect mission success. Male rodents exposed to 33-beam-GCR (33-GCR) show short-term cognitive deficits but reports on female rodents and long-term assessment is lacking. Here we asked: What are the longitudinal behavioral effects of 33-GCR on female mice? Also, can an antioxidant/anti-inflammatory compound mitigate the impact of 33-GCR? Mature (6-month-old) C57BL/6J female mice received the antioxidant CDDO-EA (400 μg/g of food) or a control diet (vehicle, Veh) for 5 days and either Sham-irradiation (IRR) or whole-body 33-GCR (0.75Gy) on the 4th day. Three-months post-IRR, mice underwent two touchscreen-platform tests: 1) location discrimination reversal (which tests behavior pattern separation and cognitive flexibility, two abilities reliant on the dentate gyrus) and 2) stimulus-response learning/extinction. Mice then underwent arena-based behavior tests (e.g. open field, 3-chamber social interaction). At the experiment end (14.25-month post-IRR), neurogenesis was assessed (doublecortin-immunoreactive [DCX+] dentate gyrus neurons). Female mice exposed to Veh/Sham vs. Veh/33-GCR had similar pattern separation (% correct to 1st reversal). There were two effects of diet: CDDO-EA/Sham and CDDO-EA/33-GCR mice had better pattern separation vs. their respective control groups (Veh/Sham, Veh/33-GCR), and CDDO-EA/33-GCR mice had better cognitive flexibility (reversal number) vs. Veh/33-GCR mice. Notably, one radiation effect/CDDO-EA countereffect also emerged: Veh/33-GCR mice had worse stimulus-response learning (days to completion) vs. all other groups, including CDDO-EA/33-GCR mice. In general, all mice show normal anxiety-like behavior, exploration, and habituation to novel environments. There was also a change in neurogenesis: Veh/33-GCR mice had fewer DCX+ dentate gyrus immature neurons vs. Veh/Sham mice. Our study implies space radiation is a risk to a female crew ′s longitudinal mission-relevant cognitive processes and CDDO-EA is a potential dietary countermeasure for space-radiation CNS risks.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

Yun,

Kiffer,

Bancroft

et al. 2024

Preprint

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“…The results demonstrated that the parameters of behavior did not change in irradiated animals 3 months after exposure: the level of anxiety was not increased, the exploratory behavior pattern was pronounced, and hippocampus-dependent memory de cits were not observed. However, in a long-term memory test, the group of animals irradiated with protons made fewer mistakes in nding a hidden target hole, which may be indicative of higher integrity of memory traces (Sorokina et al 2020). In our other study, in examining the effect of a dose of 0.7 Gy of accelerated carbon ions at total irradiation of mice, it was found 2 months after exposure that animals exhibited an altered behavioral pattern characterized by anxiety and a hippocampus-dependent memory de cit; however, there was no impairment of episodic memory.…”

Section: Introductionmentioning

confidence: 99%

Behavioral performance and microglial status in mice after moderate dose of proton irradiation

Sorokina,

Malkov,

Rozanova

et al. 2023

Radiat Environ Biophys

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The cognitive impairment is a remote effect of gamma radiation treatment of malignancies. The major part of the studies on the effect of proton irradiation (a promising alternative in the treatment of radioresistant tumors and tumors located close to critical organs) on the cognitive abilities of laboratory animals and their relation to morphological changes in the brain is rather contradictory. The aim of this study was to investigate cognitive functions and the dynamics of changes in morphological parameters of hippocampal microglial cells after proton irradiation. Two months after the cranial irradiation, 8-9-week-old male SHK mice were tested for total activity, spatial learning, as well as long-and short-term hippocampusdependent memory. To estimate the morphological parameters of microglia, the brain slices of control and irradiated animals each with different time after proton irradiation (24 h, 7 days, 1 month) were stained for microglial marker Iba-1. No changes in behavior or de cits in short-term and long-term hippocampus-dependent memory were found, but the impairment of episodic memory was observed. A change in the morphology of hippocampal microglial cells, which is characteristic of the transition of cells to an activated state, was detected. Radiation-induced oxidative stress into the brain tissue of mice one day after exposure resulted in a slight decrease in cell density, which was restored to the control level by 30 days after exposure, probably due to the activation of proliferation. The results obtained may be promising with regard to the future use of using high doses of protons per fraction in the irradiation of tumors.

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The Combined Effect of Protective Agents and Accelerated Carbon Ions on the Behavior of Mice

Сорокина

Paskevich

Заичкина

et al. 2022

Biol Bull Russ Acad Sci

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The Early Delayed Effect of Accelerated Carbon Ions and Protons on the Cognitive Functions of Mice

Cited by 5 publications

References 12 publications

The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

The longitudinal behavioral effects of acute exposure to galactic cosmic radiation in female C57BL/6J mice: implications for deep space missions, female crews, and potential antioxidant countermeasures

Behavioral performance and microglial status in mice after moderate dose of proton irradiation

The Combined Effect of Protective Agents and Accelerated Carbon Ions on the Behavior of Mice

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