2001
DOI: 10.1038/35106593
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The E2F1–3 transcription factors are essential for cellular proliferation

Abstract: The retinoblastoma tumour suppressor (Rb) pathway is believed to have a critical role in the control of cellular proliferation by regulating E2F activities. E2F1, E2F2 and E2F3 belong to a subclass of E2F factors thought to act as transcriptional activators important for progression through the G1/S transition. Here we show, by taking a conditional gene targeting approach, that the combined loss of these three E2F factors severely affects E2F target expression and completely abolishes the ability of mouse embr… Show more

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Cited by 544 publications
(503 citation statements)
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“…Compound knockout of E2Fs 1-3 leads to cell cycle arrest of mouse embryonic fibroblasts (MEFs) with an accumulation of the cdk inhibitor p21 (Wu et al, 2001). This is similar to RNAi knock-down of dE2F1 in Drosophila SL2 cells , supporting the notion of a conserved role for the activating E2F proteins.…”
Section: Cell Cycle Roles Of the Rb Family Of Proteinsmentioning
confidence: 63%
“…Compound knockout of E2Fs 1-3 leads to cell cycle arrest of mouse embryonic fibroblasts (MEFs) with an accumulation of the cdk inhibitor p21 (Wu et al, 2001). This is similar to RNAi knock-down of dE2F1 in Drosophila SL2 cells , supporting the notion of a conserved role for the activating E2F proteins.…”
Section: Cell Cycle Roles Of the Rb Family Of Proteinsmentioning
confidence: 63%
“…Mouse embryo fibroblasts (MEFs) with a defect in the E2F3 gene (E2F3À/À) have a proliferative and cell cycle defect when compared to their wild-type counterparts and at a critical threshold level, E2F3-controlled genes appear to determine the time of the G1/S transition (Leone et al, 1998;Humbert et al, 2000;Wu et al, 2001). Additionally, microinjection of E2F3 antibodies into the MEFs impairs entry into S phase (Leone et al, 1998).…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, knockout of E2F1 in mouse embryo fibroblasts or in T lymphocytes modestly slows progression from quiescence to S phase (Wang et al, 1998;Murga et al, 2001), and E2F2 À/À and E2F1/E2F2 À/À mice exhibit impaired hematopoiesis resulting from defective S-phase progression in progenitor populations (Li et al, 2003). The combined inactivation of E2F1, 2 and 3 completely abolishes entry into S phase and proliferation, along with reduced expression of E2F target genes necessary for these processes (Wu et al, 2001). These results argue for a positive role for E2F1-3 in cell cycle progression.…”
Section: Introductionmentioning
confidence: 99%