The E-Test applied to susceptibility tests of gonococci, multiply-resistant enterococci, and enterobacteriaceae producing potent β-lactamases

Sanchez, Martha L.; Barrett, Mary S.; Jones, Ronald N.

doi:10.1016/0732-8893(92)90090-g

Cited by 36 publications

(28 citation statements)

References 7 publications

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“…Etest technology is based on the diffusion of a defined continuous antimicrobial gradient from a thin plastic strip, producing an organism inhibition ellipse whose intercepts with the graded test carrier indicate MICs. 24 All the isolates of E. faecalis, 41(100%) and E. avium, 19(100%), minimum inhibitory concentration (MIC) for vancomycin were less than 2 microgram. Whereas, among the 66 isolates of E. faecium, 62 (93.94%), MIC was less than 2 microgram, while the remaining 4 (6.06%) MIC was in between 30 to 60 microgram ( Figure 6).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic speciation of enterococci with special reference to prevalence, virulence and antimicrobial resistance

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Phenotypic speciation of enterococci with special reference to prevalence, virulence and antimicrobial resistance

et al. 2015

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“…The minimum inhibitory concentration of each antibiotic against various strains employed in this study was determined by the E-test system (Sanchez et al 1992) and checkerboard microdilution techniques (Eliopulos & Moellering 1991).…”

Section: Minimum Inhibitory Concentration Determinationsmentioning

confidence: 99%

In vitro Activity of Phenothiazine Derivatives in Enterococcus faecalis and Enterococcus faecium

Hendricks

Molnár

Butterworth

et al. 2005

Basic Clin Pharma Tox

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Abstract:The antimicrobial activity of the phenothiazine derivatives thioridazine and prochlorperazine have been evaluated with 11 Enterococcus faecalis strains and 9 Enterococcus faecium strains, originating from human infections and animal faecal flora. We found that all E. faecalis and E. faecium strains, regardless of their susceptibility to commonly used antibiotics, were inhibited by thioridazine at a concentration of 16-32 mg/ml and by prochlorperazine at a concentration of 32-64 mg/ ml. Combinations of the antibiotics vancomycin or ampicillin and thioridazine and prochlorperazine at subinhibitory concentrations, could render vancomycin-or ampicillin-resistant bacteria sensitive to each of the antibiotics. Verapamil and reserpine, inhibitors of P-glycoprotein-mediated multidrug resistance, did not reduce resistance. Our results outline modification of resistance in enterococci induced by phenothiazine derivatives unrelated to P-glycoprotein-mediated multidrug resistance.

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“…E-test MIC determinations (AB BIODISK) were performed on Por-producing E. coli, the vector-bearing negative controls, and isogenic gonococcal mutants producing either P.1A or P.1B [55]. The only modification to usual E-test methods was the inclusion of ampicillin in all E. coli groups, to maintain plasmid selection.…”

Section: Methodsmentioning

confidence: 99%

Invasion of Human Fallopian Tube Epithelium byEscherichia coliExpressing Combinations of a Gonococcal Porin, Opacity‐Associated Protein, and Chimeric Lipo‐oligosaccharide

et al. 2001

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The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P<.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P<.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P<.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P<.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.

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The E-Test applied to susceptibility tests of gonococci, multiply-resistant enterococci, and enterobacteriaceae producing potent β-lactamases

Cited by 36 publications

References 7 publications

Phenotypic speciation of enterococci with special reference to prevalence, virulence and antimicrobial resistance

Phenotypic speciation of enterococci with special reference to prevalence, virulence and antimicrobial resistance

In vitro Activity of Phenothiazine Derivatives in Enterococcus faecalis and Enterococcus faecium

Invasion of Human Fallopian Tube Epithelium byEscherichia coliExpressing Combinations of a Gonococcal Porin, Opacity‐Associated Protein, and Chimeric Lipo‐oligosaccharide

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