The dynamics of GATG glycodendrimers by NMR diffusion and quantitative 13C relaxation

Novoa-Carballal, Ramón; Säwén, Elin; Fernández-Megía, Eduardo; Correa, Juan; Riguera, Ricardo; Widmalm, Göran

doi:10.1039/c003645p

Cited by 25 publications

(25 citation statements)

References 29 publications

(15 reference statements)

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“…The presence of peripheral azides on GATG dendrimers has allowed their straightforward functionalization by means of the Cu (I) catalyzed azide alkyne cycloaddition (CuAAC). The resulting dendrimers have emerged as interesting tools in the study of the multivalent carbohydrate-receptor interaction, the dynamics of dendrimers, and the preparation of polyion complex (PIC) micelles [17][18][19]. More recently, GATG-based contrast agents for magnetic resonance imaging (MRI) and as inhibitors of the dimerization of the capsid protein (CA) of HIV-1 have been also reported [20,21].…”

Section: Introductionmentioning

confidence: 99%

Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers

et al. 2012

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The synthesis of a new family of amino-functionalized gallic acid-triethylene glycol (GATG) dendrimers and their block copolymers with polyethylene glycol (PEG) has recently being disclosed. In addition, these dendrimers have shown potential for gene delivery applications, as they efficiently complex nucleic acids and form small and homogeneous dendriplexes. On this basis, the present study aimed to explore the interaction of the engineered dendriplexes with blood components, as well as their stability, cytotoxicity and ability to enter and transfect mammalian cells. Results show that GATG dendrimers can form stable dendriplexes, protect the associated pDNA from degradation, and are biocompatible with HEK-293T cells and erythrocytes. More importantly, dendriplexes are effectively internalized by HEK-293T cells, which are successfully transfected. Besides, PEGylation has a marked influence on the properties of the resulting dendriplexes. While PEGylated GATG dendrimers have improved biocompatibility, the long PEG chains limit their uptake by HEK-293T cells, and thus, their ability to transfect them. As a consequence, the degree of PEGylation in dendriplexes containing dendrimer/block copolymer mixtures emerges as an important parameter to be modulated in order to obtain an optimized stealth formulation able to effectively induce the expression of the encoded protein.

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Section: Introductionmentioning

confidence: 99%

Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers

et al. 2012

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“…9) (86), were interpreted as a radial increase of dynamics in the same direction (congested core protons surrounded by more flexible external nuclei). This dynamical picture was later confirmed under more relevant aqueous conditions by a quantitative 13 C relaxation study in collaboration with the group of Widmalm (87).…”

Section: Dendrimer Dynamicsmentioning

confidence: 73%

“…In general, for NMR studies on the dynamics of macromolecules, quantitative modeling from 13 C relaxation is preferred to 1 H. However, lengthy 13 C experiments and the necessity of recording various parameters at different magnetic fields have limited such approach in dendrimers to a single report apart from ours (87,88). Conversely, a great deal of information has been extracted by qualitative interpretation of 1 H and/or 13 C relaxation.…”

Section: Dendrimer Dynamicsmentioning

confidence: 99%

GATG Dendrimers and PEGylated Block Copolymers: from Synthesis to Bioapplications

Sousa-Hervés

Novoa-Carballal

Riguera

et al. 2014

AAPS J

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Dendrimers are synthetic macromolecules composed of repetitive layers of branching units that emerge from a central core. They are characterized by a tunable size and precise number of peripheral groups which determine their physicochemical properties and function. Their high multivalency, functional surface, and globular architecture with diameters in the nanometer scale makes them ideal candidates for a wide range of applications. Gallic acid-triethylene glycol (GATG) dendrimers have attracted our attention as a promising platform in the biomedical field because of their high tunability and versatility. The presence of terminal azides in GATG dendrimers and poly(ethylene glycol) (PEG)-dendritic block copolymers allows their efficient functionalization with a variety of ligands of biomedical relevance including anionic and cationic groups, carbohydrates, peptides, or imaging agents. The resulting functionalized dendrimers have found application in drug and gene delivery, as antiviral agents and for the treatment of neurodegenerative diseases, in diagnosis and as tools to study multivalent carbohydrate recognition and dendrimer dynamics. Herein, we present an account on the preparation and recent applications of GATG dendrimers in these fields.

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“…We found a few experimental studies in which the local orientational mobility of dendrimers in a dilute solution of chloroform was studied. 34,37 Novoa-Carballal et al 37 studied GATG 1-, 2-and 3-generation dendrimers using an NMR technique. The 13 C NMR spin-lattice relaxation time, T 1C , was particularly studied in detail for different carbon atoms.…”

Section: Resultsmentioning

confidence: 99%

Average relaxation time of internal spectrum for carbosilane dendrimers: Nuclear magnetic resonance studies

Markelov

Матвеев

Ingman

et al. 2011

The Journal of Chemical Physics

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A new theoretical description of the interior mobility of carbosilane dendrimers has been tested. Experiments were conducted using measurements of the (1)H NMR spin-lattice relaxation time, T(1H), of two-, three- and four-generation carbosilane dendrimers with three different types of terminal groups in dilute chloroform solutions. Temperature dependences of the NMR relaxation rate, 1/T(1H), were obtained for the internal CH(2)-groups of the dendrimers in the range of 1/T(1H) maximum, allowing us to directly evaluate the average time of the internal spectrum for each dendrimer. It was found that the temperature of 1/T(1H) maximum is practically independent of the number of generations, G; therefore, the theoretical prediction was confirmed experimentally. In addition, the average time of the internal spectrum of carbosilane dendrimers was found to be near 0.2 ns at room temperature, and this value correlates well with the values previously obtained for other dendrimer structures using other experimental techniques.

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The dynamics of GATG glycodendrimers by NMR diffusion and quantitative 13C relaxation

Abstract: The dynamics of GATG glycodendrimers have been investigated by NMR translational diffusion and quantitative (13)C relaxation studies (Lipari-Szabo model-free), allowing the determination of the correlation times describing the dendrimer segmental orientational mobility.

Cited by 25 publications

References 29 publications

Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers

Exploring the efficiency of gallic acid-based dendrimers and their block copolymers with PEG as gene carriers

GATG Dendrimers and PEGylated Block Copolymers: from Synthesis to Bioapplications

Average relaxation time of internal spectrum for carbosilane dendrimers: Nuclear magnetic resonance studies

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