The dynamics of clot formation in fresh‐frozen plasma

Lawrie, Andrew; Cardigan, Rebecca; Williamson, Lorna M.; Machin, Samuel J.; Mackie, Ian

doi:10.1111/j.1423-0410.2008.01037.x

Cited by 26 publications

(32 citation statements)

References 32 publications

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“…There are several studies documenting clinical relevance of MPs in supporting coagulation [32-38]. We and others have previously shown that PMPs, generated during extended blood storage, contribute to functional hemostasis in stored plasma and correlate with enhanced thrombin generation [12, 39]. However, since MPs are heterogenous and contain many proteins with opposing activities (pro- and anti-coagulants, proteases and their inhibitors), more detailed studies are needed to elucidate their specific roles in severe trauma with coagulopathy.…”

Section: Discussionmentioning

confidence: 99%

Cellular microparticle and thrombogram phenotypes in the Prospective Observational Multicenter Major Trauma Transfusion (PROMMTT) Study: Correlation with coagulopathy

Matijevic

Wang

Wade

et al. 2014

Thrombosis Research

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Background Trauma-induced coagulopathy following severe injury is associated with increased bleeding and mortality. Injury may result in alteration of cellular phenotypes and release of cell-derived microparticles (MP). Circulating MPs are procoagulant and support thrombin generation (TG) and clotting. We evaluated MP and TG phenotypes in severely injured patients at admission, in relation to coagulopathy and bleeding. Methods As part of the Prospective Observational Multicenter Major Trauma Transfusion (PROMMTT) study, research blood samples were obtained from 180 trauma patients requiring transfusions at 5 participating centers. Twenty five healthy controls and 40 minimally injured patients were analyzed for comparisons. Laboratory criteria for coagulopathy was activated partial thromboplastin time (APTT) ≥35 sec. Samples were analyzed by Calibrated Automated Thrombogram to assess TG, and by flow cytometry for MP phenotypes [platelet (PMP), erythrocyte (RMP), leukocyte (LMP), endothelial (EMP), tissue factor (TFMP), and Annexin V positive (AVMP)]. Results 21.7% of patients were coagulopathic with the median (IQR) APTT of 44 sec (37, 53), and an Injury Severity Score of 26 (17, 35). Compared to controls, patients had elevated EMP, RMP, LMP, and TFMP (all p<0.001), and enhanced TG (p<0.0001). However, coagulopathic PROMMTT patients had significantly lower PMP, TFMP, and TG, higher substantial bleeding, and higher mortality compared to non-coagulopathic patients (all p<0.001). Conclusions Cellular activation and enhanced TG are predominant after trauma and independent of injury severity. Coagulopathy was associated with lower thrombin peak and rate compared to non-coagulopathic patients, while lower levels of TF-bearing PMPs were associated with substantial bleeding.

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Section: Discussionmentioning

confidence: 99%

Cellular microparticle and thrombogram phenotypes in the Prospective Observational Multicenter Major Trauma Transfusion (PROMMTT) Study: Correlation with coagulopathy

Matijevic

Wang

Wade

et al. 2014

Thrombosis Research

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“…While classical quality parameters like FVIII are based on the determination of a single coagulation factor only, thrombin generation assays might be a valuable indicator for the overall hemostatic potential of FFP. However, to date there is only very limited data on thrombin generation in FFP [13,14]. Lawrie et al [13] assessed thrombin generation in FFP prepared under different conditions.…”

Section: Discussionmentioning

confidence: 99%

“…However, to date there is only very limited data on thrombin generation in FFP [13,14]. Lawrie et al [13] assessed thrombin generation in FFP prepared under different conditions. Also Pock et al [14] determined thrombin generation parameters in FFP.…”

Section: Discussionmentioning

confidence: 99%

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Thrombin Generation Capacity of Methylene Blue-Treated Plasma Prepared by the Theraflex MB Plasma System

Gravemann

Kusch

Koenig

et al. 2009

Transfus Med Hemother

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Background: Methylene blue (MB) / light treatment is a well-known procedure for the inactivation of pathogens in fresh frozen plasma (FFP). Aim of the current study was to investigate the thrombin generation (TG) characteristics and quality of MB plasma prepared by the Theraflex MB Plasma System. Methods: Single donor plasma units (n = 18) were MB/light-treated, with sampling before and after processing. Preparation included leukocyte depletion, addition of MB pill prior to illumination, and depletion of MB and photoproducts by filtration. Different plasma parameters and TG were measured. TG additionally was determined in solvent/detergent plasma (n = 8). Results: MB/light treatment significantly affected factors V, VIII and XI, which were decreased by 9–18%. While the antigen level was not affected, fibrinogen according to Clauss was decreased by 7%, correlating with a 12% prolongation of TT and RT. The total amount of free thrombin generated, given as ‘area under the curve’ (AUC), was comparable for untreated (93 ± 18% of normal plasma) and MB/light-treated plasma (95 ± 20%). Also peak thrombin concentration was not significantly affected by treatment (94 ± 11% (untreated) vs. 96 ± 12% (treated)). The ‘time to peak’ value (TTP) was 105% of normal plasma for untreated FFP and 89% for MB-treated plasma. Conclusion: For plasma treated with the Theraflex MB Plasma System no profound influence of MB/ light treatment on the characteristics of thrombin generation was detected. In concordance with data from the literature, coagulation factors V, VIII and XI were decreased due to MB/ light treatment. Decrease was less than 20%.

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“…Recently, miRNA-21 has been found to be overexpressed in diffuse large B cell lymphoma and is validated as an independent risk factor in prognosis [10–12]. miRNA-21 is also a diagnostic biomarker in primary diffuse large B cell lymphoma of the central nervous system [13].…”

Section: Introductionmentioning

confidence: 99%

Circulating MicroRNA-21, MicroRNA-23a, and MicroRNA-125b as Biomarkers for Diagnosis and Prognosis of Burkitt Lymphoma in Children

Zhai

Wang

et al. 2016

Med Sci Monit

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BackgroundThe aim of this study was to investigate the diagnostic and prognostic value of microRNA (miRNA)-21, miRNA-23a, and miRNA-125b in Burkitt lymphoma (BL) in children.Material/MethodsWe recruited 41 children with BL for the case group, 56 children with lymph node inflammation for the positive control group, and 60 healthy children for the negative control group. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was conducted for detection of circulating miRNA-21, miRNA-23a, and miRNA-125b. A receiver operating characteristic (ROC) curve was drawn to compare the diagnostic value of miRNA-21, miRNA-23a, and miRNA-125b. Kaplan-Meier method and log-rank test were used for prognostic analyses.ResultsMiRNA-21 and miRNA-23a had significantly higher expression in cases than in positive and negative controls (all P<0.05). Overexpression of miRNA-21 and miRNA-23a were associated with staging, WBC, upregulated serum lactate dehydrogenase (LDH) level, presence of lymphoma size ≥6 cm, and cluster of differentiation 10 (CD10) expression, while miRNA-125b expression had an association with staging and upregulated serum LDH level (both P<0.05). ROC curves of miRNA-21, miRNA-23a, and miRNA-125b presented an area under curve (AUC) of 0.759, 0.853 and 0.615, respectively. MiRNA-21 and miRNA-23a in combination had an AUC of 0.869. After treatment, both miRNA-21 and miRNA-23a expression were significantly decreased (both P<0.05). Advanced clinical stage, upregulated LDH, and lymphoma size of ≥6 cm were related to low complete remission rate (all P<0.05).ConclusionsPatients with high expression of miRNA-21 and miRNA-23a had significantly lower complete remission rates and survival rates than those with low expression. Expression of miRNA-21 and miRNA-23a may serve as useful diagnostic and prognostic biomarkers in children with BL.

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The dynamics of clot formation in fresh‐frozen plasma

Abstract: We conclude that levels of FVIII do not predict the haemostatic potential of FFP and that there may be a role for alternative technologies in monitoring the quality of FFP.

Cited by 26 publications

References 32 publications

Cellular microparticle and thrombogram phenotypes in the Prospective Observational Multicenter Major Trauma Transfusion (PROMMTT) Study: Correlation with coagulopathy

Cellular microparticle and thrombogram phenotypes in the Prospective Observational Multicenter Major Trauma Transfusion (PROMMTT) Study: Correlation with coagulopathy

Thrombin Generation Capacity of Methylene Blue-Treated Plasma Prepared by the Theraflex MB Plasma System

Circulating MicroRNA-21, MicroRNA-23a, and MicroRNA-125b as Biomarkers for Diagnosis and Prognosis of Burkitt Lymphoma in Children

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