“…small vibrations), as classified in the Matryoshka model recently proposed for a very complete description of motions within a membrane. 28 The behaviour of G as function of Q 2 is indicative for the type of movement: 29 a linear behaviour represents translational diffusion characterized by the diffusion coefficient D T :…”
“…The C10mix seems again the most flexible, but very similar to its counterpart with eicosane. The amplitudes A 0 to A 3 will be treated in more detail with the Matryoshka model 28 to extract also structural information (see below).…”
“…small vibrations), as classified in the Matryoshka model recently proposed for a very complete description of motions within a membrane. 28 The behaviour of G as function of Q 2 is indicative for the type of movement: 29 a linear behaviour represents translational diffusion characterized by the diffusion coefficient D T :…”
“…The C10mix seems again the most flexible, but very similar to its counterpart with eicosane. The amplitudes A 0 to A 3 will be treated in more detail with the Matryoshka model 28 to extract also structural information (see below).…”
“…In the dynamical analysis of apo B-100 in complex with NP40, a recently published dynamical model called the Matryoshka model [ 15 , 16 , 78 ] was employed with some modifications. The Matryoshka model takes into account various kinds of intramolecular motions manifested in phospholipids in addition to the diffusion of the entire molecule.…”
Section: Ins Studies On Pathologically Relevant Bio-macromoleculesmentioning
confidence: 99%
“… Hierarchical intramolecular motions observed by iNS for proteins ( a ) and lipid molecules ( b ). Classification of the motions of lipid molecules is based on the latest theoretical dynamical model called the Matryoshka model [ 15 ]. This figure is adapted from Ref.…”
Incoherent neutron scattering (iNS) is one of the most powerful techniques to study the dynamical behavior of bio-macromolecules such as proteins and lipid molecules or whole cells. This technique has widely been used to elucidate the fundamental aspects of molecular motions that manifest in the bio-macromolecules in relation to their intrinsic molecular properties and biological functions. Furthermore, in the last decade, iNS studies focusing on a possible relationship between molecular dynamics and biological malfunctions, i.e., human diseases and disorders, have gained importance. In this review, we summarize recent iNS studies on pathologically relevant proteins and lipids and discuss how the findings are of importance to elucidate the molecular mechanisms of human diseases and disorders that each study targets. Since some diseases such as amyloidosis have become more relevant in the aging society, research in this field will continue to develop further and be more important in the current increasing trend for longevity worldwide.
“…This approach is directly derived from a model for lipid motions called the Matryoshka model. 46 In the case of apo B-100/NP40, our model considers that apo B-100 domains and NP40 chains undergo distinct confined diffusion 47 while at the same time their methyl groups perform jump diffusion. 35,48 Jump diffusion is defined as a diffusion within distinct sites and "jumps" between these sites after a certain time interval.…”
Apolipoprotein B-100 (apo B-100) is the protein moiety of both low-and verylow-density lipoproteins, whose role is crucial to cholesterol and triglyceride transport. Aiming at the molecular dynamics' details of apo B-100, scarcely studied, we performed elastic and quasi-elastic incoherent neutron scattering (EINS, QENS) experiments combining different instruments and time scales. Similar to classical membrane proteins, the solubilization results in remaining detergent, here Nonidet P-40 (NP40). Therefore, we propose a framework for QENS studies of protein−detergent complexes, with the introduction of a combined model, including the experimental apo B-100/NP40 ratio. Relying on the simultaneous analysis of all QENS amplitudes, this approach is sensitive enough to separate both contributions. Its application identified two points: (i) apo B-100 slow dynamics and (ii) the acceleration of NP40 dynamics in the presence of apo B-100. Direct translation of the exposed methodology now makes the investigation of more membrane proteins by neutron spectroscopy achievable.
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