The Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles

Zhang, Qi; Li, Yulong; Tsien, Richard W.

doi:10.1126/science.1167373

Cited by 314 publications

(378 citation statements)

References 41 publications

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“…In dopaminergic neurons, the neurons vulnerable in Parkinson's disease, kiss-and-run-mediated exocytosis has been clearly shown [58,59]. However, in hippocampal neurons some studies argue that clathrin-mediated endocytosis is the main or even the only mechanism [54], while others report up to 60% of kiss-and-run endocytosis [60]. Clathrin-mediated endocytosis is one major mechanism of endocytosis, but there is still controversy on the involvement and relative importance of other mechanisms during 'normal' synaptic vesicle endocytosis, and it is not yet known if there are variations for different neuronal subtypes.…”

Section: Mechanisms Of Synaptic Vesicle Endocytosismentioning

confidence: 99%

α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?

Lautenschläger

Kaminski

Schierle

2017

Trends in Cell Biology

121

124

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a-Synuclein is known as a presynaptic protein that binds to small synaptic vesicles. Recent studies suggest that a-synuclein is not only attracted to these tiny and therewith highly curved membranes, but that in fact the sensing and regulation of membrane curvature is part of its physiological function. Moreover, recent studies have suggested that a-synuclein plays a role in the endocytosis of synaptic vesicles, and have provided support for a function of a-synuclein during exo-and endocytosis in which curvature sensing and membrane stabilization are crucial steps. This review aims to highlight recent research in the field and adds a new picture on the function of a-synuclein in maintaining synaptic homeostasis upon intense and repetitive neuronal activity.

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Section: Mechanisms Of Synaptic Vesicle Endocytosismentioning

confidence: 99%

α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?

Lautenschläger

Kaminski

Schierle

2017

Trends in Cell Biology

121

124

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“…Different FM dyes exhibit distinct partitioning coefficients and wavelengths that allow for probing the system. Recently, quantum dots have been introduced as an alternative tracer to monitor fusion events 116 . A distinct advantage of such live tracers is the fact that they can be applied directly to preparations without a need for genetic manipulation or transfection.…”

Section: Box 3 | Visualizing Presynaptic Exocytosis and Endocytosismentioning

confidence: 99%

Protein scaffolds in the coupling of synaptic exocytosis and endocytosis

2011

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Communication between nerve cells largely occurs at chemical synapses -specialized sites of cell-cell contact where electrical signals trigger the exocytic release of neurotransmitter, which in turn activates postsynaptic receptor channels. The efficacy with which such chemical signals are transmitted is crucial for the functioning of the nervous system. Modulation of neurotransmission enables nerve cells to respond to a vast range of stimulus patterns. Synaptic activity can also be tremendously diverse; from the fast synapses of the hippocampus, to slow neuropeptide-containing synapses. Indeed, some signalling pathways are active in the absence of stimulation, such as those involved in the processing of sensory information, which transmit tonically at high rates of up to 100 Hz or more 1,2 .Intriguing questions arise from these facts, including how high rates of neurotransmission can be maintained over long periods of time, and what limits the ability of a given synapse to release neurotransmitter during sustained periods of activity. Recent data indicate that in many synapses, exocytosis of neurotransmitter is coupled to endocytosis, and that synapses have evolved a specialized apparatus of scaffolding proteins to comply with these demands. Such scaffolds aid the temporal and spatial coupling of exocytosis and endocytosis, and are crucial for maintaining rapid neurotransmission during sustained activity 3 .Exocytosis of neurotransmitter is triggered by stimulusinduced calcium influx into the nerve terminal 4,5 and the subsequent fusion of synaptic vesicles with the presynaptic plasma membrane at specialized regions called active zones (BOX 1). Exocytosed synaptic vesicle membrane proteins and lipids in turn are recycled at the endocytic or periactive zone (BOX 1) that surrounds the release site, to restore functional synaptic vesicle pools for reuse and to ensure long-term functionality of the synapse 6-8 (FIG. 1). Depending on the type of synapse and the stimulation frequency, several modes of endocytosis with different time constants -for example, fast and slow endocytosisseem to operate 7,9,10 . Clathrin-mediated endocytosis arguably represents the main pathway of synaptic vesicle endocytosis 6,8,11 , although other modes -for example, fast kiss-and-run exocytosis and endocytosis -could operate in parallel.Although the machineries for exocytic membrane fusion 12,13 and for endocytic retrieval of synaptic vesicle membranes have been studied separately in some detail 6,14 , comparatively little is known about the coupling between exocytosis and endocytosis. Here we synthesize recent data from physiological, morphological and biochemical studies in several model systems into hypothetical models for scaffold-based mechanisms underlying exocytic-endocytic coupling.The synaptic vesicle cycle Synaptic vesicle pools. Some defining features of chemical synapses are the presence of one or several clusters of synaptic vesicles in the presynaptic nerve terminal, and ultrastructural specializations at the pre-and postsy...

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“…This presents a challenge because synapses are small with complex nanoscale morphology which is not readily resolvable using conventional light microscopy. Elegant methods relying on nano-particles such as quantum dots 19,20 and super-resolution fluorescence imaging 8 provide important strategies to address this challenge, but are still limited in spatial information and ultrastructural context. An alternative approach is to combine a functional measure of vesicle pools with subsequent ultrastructural investigation.…”

Section: Labelling and Visualizing Functional Vesiclesmentioning

confidence: 99%

Ultrastructural readout of functional synaptic vesicle pools in hippocampal slices based on FM dye labeling and photoconversion

et al. 2014

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(2014) Ultrastructural readout of functional synaptic vesicle pools in hippocampal slices based on FM dye labeling and photoconversion. Nature Protocols, 9 (6). pp. 1337 -1347 . ISSN 1754 -2189 This version is available from Sussex Research Online: http://sro.sussex.ac.uk/60182/ This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published version. Copyright and reuse:Sussex Research Online is a digital repository of the research output of the University.Copyright and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable, the material made available in SRO has been checked for eligibility before being made available.Copies of full text items generally can be reproduced, displayed or performed and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way.

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The Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles

Cited by 314 publications

References 41 publications

α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?

α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?

Protein scaffolds in the coupling of synaptic exocytosis and endocytosis

Ultrastructural readout of functional synaptic vesicle pools in hippocampal slices based on FM dye labeling and photoconversion

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