The Duration of Differentiation in Excised Anthers

Taylor, J. Herbert

doi:10.1002/j.1537-2197.1950.tb08170.x

Cited by 37 publications

(16 citation statements)

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“…In other experiments, meiocytes in cultured anthers were able to undergo normal meiosis from the late meiotic prophase until the binucleate pollen stage (Sparrow et al 1955) in a medium that is quite similar to the medium used for the maturation of microspores (Benito Moreno et al 1988a). Earlier stages either failed to enter meiosis or showed highly abnormal behaviour (Gregory 1940;Taylor 1950). The critical stage appears to be the pachytene stage (Vasil 1959).…”

Section: In Vitro Maturation Of Pollenmentioning

confidence: 99%

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Heberle‐Bors

1989

Sexual Plant Reprod

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In the past, in vitro cultures of excised anthers and isolated pollen have been used to study normal male sexual development (gametophytic development) and, conversely, to produce and study haploid plant formation (sporophytic development). For years both branches have existed side by side, without much interaction. Today, a synthesis of the two branches is possible as well as necessary. Recent advances in the technique of isolated pollen culture in the tobacco plant model (Nicotiana tabacum L.) enable the researcher to strictly control pollen development in both the gametophytic and sporophytic direction. The nutritional status of the immature pollen grain at a particular stage of development provides the trigger for its development into one of the two phases found in the alternation of generations undergone by higher plants. In particular, a hunger signal is responsible for the derepression of cell division activity and the start of embryogenesis. Pollen starvation can occur in isolated pollen cultures in sucrose-free media, in excised anthers and flowers, and, under specific growth conditions, during pollen development in vivo.

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Section: In Vitro Maturation Of Pollenmentioning

confidence: 99%

Isolated pollen culture in tobacco: plant reproductive development in a nutshell

Heberle‐Bors

1989

Sexual Plant Reprod

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“…The technique involved sampling single anthers or anther segments from inflorescences, recording the stage of meiosis in the PMC and subsequently sampling the remaining anthers or anther segments from the same inflorescences after specific time intervals (repeated anther sampling). Other methods based on direct cytological observations involved in vitro culturing of anthers or PMC, (Lima-de-Faria 1950;Taylor 1950;Ito and Stern 1967).…”

Section: Introductionmentioning

confidence: 99%

A meiotic time-course for Arabidopsis thaliana

Armstrong

Franklin

Jones

2003

Sexual Plant Reproduction

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A meiotic time-course for Arabidopsis pollen mother cells has been established based on BrdU pulselabelling of nuclear DNA in the meiotic S-phase. Labelled flower buds were sampled at intervals and the progress of labelled cells through meiosis assessed by anti-BrdU antibody detection. The overall duration of meiosis from the end of meiotic S-phase to the tetrad stage, at 18.5C, was 33 h, which is about three times longer than the mitotic cell cycle in seedlings. The onset of leptotene was defined by reference to the loading of the axis-associated protein Asy1, and this permitted the detection of a definite G2 stage, having a maximum duration of 9 h. It is likely, from two independent sources of evidence, that the meiotic S-phase has a duration similar to that of G2. The durations of leptotene and zygotene/pachytene are 6 h and 15.3 h, respectively, but the remaining meiotic division stages are completed very rapidly, within 3 h. The establishment of a meiotic time-course provides a framework for determining the relative timing and durations of key molecular events of meiosis in Arabidopsis in relation to cytologically defined landmarks. In addition, it will be important in a broader developmental context for determining the timing of epigenetic mechanisms that are known or suspected to occur during meiosis.

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“…However, the situation in vitro cultures seems to vary from that of vivo . According to Taylor (1950) the initiation of meiosis does not insure its completion in anthers excised in early leptotene; nor does the completion of meiosis ensure that subsequent stages in sporogenesis or pollen differentiation will all be completed in culture (Vasil 1957 and1959). On Osmunda Clatter and Sussex (1965) have reported certain stages such as the deposition of exine material on the spore surface may be modified or omitted without adversely affecting the subsequent stages of development.…”

Section: Discussionmentioning

confidence: 99%