The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids

Горкун, А.А.; Revokatova, Daria P; Зурина, И.М.; Никишин, Д. А.; Bikmulina, Polina; Timashev, Peter; Shpichka, A.I.; Kosheleva, Nastasia; Kolokoltsova, T.D.; Сабурина, И.Н.

doi:10.3389/fcell.2021.572727

Cited by 16 publications

(7 citation statements)

References 61 publications

(41 reference statements)

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“…Human samples were obtained from healthy adults from the dental pulp (bone marrow and adipose tissue) of clinically healthy exfoliated deciduous teeth or maternal cord blood under protocols approved by the ethical review board at the respective institutions. Bone marrow derived (BM), umbilical cord blood derived (UC), adipose tissue derived (AD), and dental pulp derived (DP) MSCs (BM-MSCs, AD-MSCs, UC-MSCs, and DP-MSCs, respectively) were isolated as previously described [ 27 , 28 , 29 , 30 ]. The cells’ cultural and morphological properties, the expression of surface receptors, and the ability to differentiate were characterized.…”

Section: Methodsmentioning

confidence: 99%

Human Mesenchymal Stem Cells Modified with the NS5A Gene of Hepatitis C Virus Induce a Cellular Immune Response Exceeding the Response to DNA Immunization with This Gene

Масалова

Леснова

Kalsin

et al. 2023

Biology

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Hepatitis C virus (HCV) is one of the basic culprits behind chronic liver disease, which may result in cirrhosis and hepatocarcinoma. In spite of the extensive research conducted, a vaccine against HCV has not been yet created. We have obtained human mesenchymal stem cells (hMSCs) and used them for expressing the HCV NS5A protein as a model vaccination platform. Sixteen hMSC lines of a different origin were transfected with the pcNS5A-GFP plasmid to obtain genetically modified MSCs (mMSCs). The highest efficiency was obtained by the transfection of dental pulp MSCs. C57BL/6 mice were immunized intravenously with mMSCs, and the immune response was compared with the response to the pcNS5A-GFP plasmid, which was injected intramuscularly. It was shown that the antigen-specific lymphocyte proliferation and the number of IFN-γ-synthesizing cells were two to three times higher after the mMSC immunization compared to the DNA immunization. In addition, mMSCs induced more CD4+ memory T cells and an increase in the CD4+/CD8+ ratio. The results suggest that the immunostimulatory effect of mMSCs is associated with the switch of MSCs to the pro-inflammatory phenotype and a decrease in the proportion of myeloid derived suppressor cells. Thus, the possibility of using human mMSCs for the creation of a vaccine against HCV has been shown for the first time.

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Section: Methodsmentioning

confidence: 99%

Human Mesenchymal Stem Cells Modified with the NS5A Gene of Hepatitis C Virus Induce a Cellular Immune Response Exceeding the Response to DNA Immunization with This Gene

Масалова

Леснова

Kalsin

et al. 2023

Biology

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“…After centrifugation, ASCs contained in the SVF can be successively expanded for further uses. Based on cell-cell and cell-matrix interactions, 3D cultures of ASCs would offer a favorable context for their multilineage vascular and osteogenic differentiation, providing in the resulting spheroids an in vitro model of the interactive elements [54]. This strategy is particularly advantageous to improve the thickness of a scaffold-free implant.…”

Section: Asc-based Repair Strategiesmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stromal Cells: A Tool for Bone and Cartilage Repair

et al. 2023

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The osteogenic and chondrogenic differentiation ability of adipose-derived mesenchymal stromal cells (ASCs) and their potential therapeutic applications in bone and cartilage defects are reported in this review. This becomes particularly important when these disorders can only be poorly treated by conventional therapeutic approaches, and tissue engineering may represent a valuable alternative. Being of mesodermal origin, ASCs can be easily induced to differentiate into chondrocyte-like and osteocyte-like elements and used to repair damaged tissues. Moreover, they can be easily harvested and used for autologous implantation. A plethora of ASC-based strategies are being developed worldwide: they include the transplantation of freshly harvested cells, in vitro expanded cells or predifferentiated cells. Moreover, improving their positive effects, ASCs can be implanted in combination with several types of scaffolds that ensure the correct cell positioning; support cell viability, proliferation and migration; and may contribute to their osteogenic or chondrogenic differentiation. Examples of these strategies are described here, showing the enormous therapeutic potential of ASCs in this field. For safety and regulatory issues, most investigations are still at the experimental stage and carried out in vitro and in animal models. Clinical applications have, however, been reported with promising results and no serious adverse effects.

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“…For AT-MSCs isolation the previously reported protocols 23,24 were adjusted as follows: abdominal subcutaneous adipose tissue biopsy samples were thoroughly washed with Hanks' solution (BioloT, Russia) supplemented with Di ucan (P zer, USA), gentamicin (50 µg/mL, PanEco, Russia) and penicillin/streptomycin (100 U/ml/100 µg/ml, PanEco, Russia). Each sample was minced into small (~ 1 mm 3 ) pieces with scissors and transferred into conical tubes.…”

Section: Mscs Sourcesmentioning

confidence: 99%

MSCs’ conditioned media cytokine and growth factor profiles and their impact on macrophage polarization

Peshkova

Корнеев

Сулейманов

et al. 2023

Preprint

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Background. There is a growing body of evidence that multipotent mesenchymal stromal cells’ (MSCs’) remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. MSCs’ secretome (i.e., cytokines, growth factors, and extracellular vesicles) is therefore increasingly discussed in the context of their ability to modulate inflammatory response and promote regeneration. There is evidence that 2D or 3D culturing conditions have an impact on the cells’ secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. Methods. MSCs were derived from human adipose tissue, bone marrow, gingiva, placenta, and umbilical cord, cultured as monolayers or as cell spheroids. Their cytokine profiles were analyzed, and data standardization was carried out using a z-score. Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs’ conditioned media and their effect on macrophages polarization was assessed. Results. Our findings suggest that umbilical cord-derived MSCs’ conditioned media demonstrated the highest cytokine and growth factor levels and despite mostly pro-inflammatory cytokine profile were able to promote human macrophages’ polarization to anti-inflammatory M2 state and inhibit their polarization to pro-inflammatory M1 state. Conclusions. Umbilical cord-derived MSCs’ conditioned media hold great potential for therapeutic use, demonstrating significant anti-inflammatory effect on human macrophages.

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The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids

Abstract: Graphical Abstract

Cited by 16 publications

References 61 publications

Human Mesenchymal Stem Cells Modified with the NS5A Gene of Hepatitis C Virus Induce a Cellular Immune Response Exceeding the Response to DNA Immunization with This Gene

Human Mesenchymal Stem Cells Modified with the NS5A Gene of Hepatitis C Virus Induce a Cellular Immune Response Exceeding the Response to DNA Immunization with This Gene

Adipose-Derived Mesenchymal Stromal Cells: A Tool for Bone and Cartilage Repair

MSCs’ conditioned media cytokine and growth factor profiles and their impact on macrophage polarization

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