The DT40 web site: sampling and connecting the genes of a B cell line

Buerstedde, Jean-Marie; Arima, Hiroshi; Watahiki, Akira; Carninci, Piero; Hayashizaki, Yoshihide; Korn, Bernhard; Plachý, Jiřı́

doi:10.1093/nar/30.1.230

Cited by 26 publications

(19 citation statements)

References 14 publications

(9 reference statements)

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“…Chicken EST databases (12,53) were screened by BLAST with human TLR5 (huTLR5) sequences (NM003268). Primers were designed from a putative chTLR5 EST and used to generate amplification products from genomic DNA for hybridization screening of the Wageningen Bacterial Artificial Chromosome (BAC) library (14) with filters supplied by the Medical Research Council Human Genome Mapping Project Resource Centre, Cambridge, United Kingdom.…”

Section: Methodsmentioning

confidence: 99%

Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium

Iqbal¹,

Philbin²,

et al. 2005

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Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In chickens, the differential biology of Salmonella serovars (systemic versus gut-restricted localization) correlates with the presence or absence of flagella, a known TLR5 agonist. Chicken TLR5 (chTLR5) exhibits conserved sequence and structural similarity with mammalian TLR5 and is expressed in tissues and cell populations of immunological and stromal origin. Exposure of chTLR5 ؉ cells to flagellin induced elevated levels of chicken interleukin-1␤ (chIL-1␤) but little upregulation of chIL-6 mRNA. Consistent with the flagellin-TLR5 hypothesis, an aflagellar Salmonella enterica serovar Typhimurium fliM mutant exhibited an enhanced ability to establish systemic infection. During the early stages of infection, the fliM mutant induced less IL-1␤ mRNA and polymorphonuclear cell infiltration of the gut. Collectively, the data represent the identification and functional characterization of a nonmammalian TLR5 and indicate a role in restricting the entry of flagellated Salmonella into systemic sites of the chicken.The pattern recognition receptors (PRRs) play a central role in the rapid initiation of host immune responses and the generic identification of an invading pathogen (36, 43) by recognition of pathogen-associated molecular patterns. Toll-like receptors (TLRs) have emerged as a major component of the vertebrate PRR repertoire. Upon activation, TLRs induce the expression of a wide range of immunoregulatory and effector molecules (41, 51) and maturation of immune cell types (1,3,11,24,50).A range of TLR genes has been identified in nonmammalian vertebrates including chicken (10, 18, 32) and fish (6,26,37). To date, avian orthologues of TLR2 and TLR4 have been characterized and expressed sequence tags (ESTs) with sequence homologies to TLR1, -6, or -10; TLR3; TLR5; and TLR7 have been identified (34, 48; our unpublished results). Two chicken TLR2 (chTLR2) molecules (type 1 and type 2) were identified that lie in a tandem arrangement within a genomic region expressing conserved synteny to mammals (10, 18). The chTLR4 gene was also demonstrated to lie in a region of conserved synteny and has been associated with susceptibility to systemic infection with Salmonella enterica serovar Typhimurium in young chickens (32). Collectively, these data indicate that a range of distinct TLR genes, orthologous to the mammalian TLR repertoire, were present before the divergence of birds and mammals over 300 million years ago.The observation that nonflagellated Salmonella enterica serovars (Gallinarum or Pullorum) typically cause more acute systemic infection than flagellated serovars (Typhimurium or Enteritidis) provoked our interest in chTLR5. Our working hypothesis was that TLR5-flagellin interactions contribute to the broad biology of Salmonella serovars in the chicken. We identified a chicken orthologue for TLR5, determined expression ...

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Section: Methodsmentioning

confidence: 99%

Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium

Iqbal¹,

Philbin²,

et al. 2005

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“…Data thus obtained can be of great help for building the more detailed maps based on large insert BAC clone contigs (Lee et al, 2001;Jennen et al, 2004). The mapping of genes is also greatly facilitated, as compared to genetic mapping, an important point to be taken into consideration with the large amount of EST data now available (Abdrakhmanov et al, 2000;Tirunagaru et al, 2000;Carre et al, 2001;Boardman et al, 2002;Buerstedde et al, 2002).…”

Section: Construction Of the Gga10 Rh Mapmentioning

confidence: 99%

Second report on chicken genes and chromosomes 2005

Schmid

Nanda

Hoehn

et al. 2005

Cytogenet Genome Res

149

167

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“…Chicken EST clones homologous to human Rad17 and Rad9 proteins were searched for using the blast program in the chicken bursal EST database (Buerstedde et al 2002). Oligonucleotide PCR primers were designed based on the identified chicken Rad17 (riken1 16b18r1.blsp) and Rad9 (dkfz426 5C5r1.blsp) EST clones, and partial chicken rad17 and rad9 cDNAs were amplified by RT-PCR using mRNA extracted from DT40 cells.…”

Section: Gene Targetingmentioning

confidence: 99%

Critical role for chicken Rad17 and Rad9 in the cellular response to DNA damage and stalled DNA replication

et al. 2004

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The Rad17-replication factor C (Rad17-RFC) and Rad9-Rad1-Hus1 complexes are thought to function in the early phase of cell-cycle checkpoint control as sensors for genome damage and genome replication errors. However, genetic analysis of the functions of these complexes in vertebrates is complicated by the lethality of these gene disruptions in embryonic mouse cells. We disrupted the Rad17 and Rad9 loci by gene targeting in the chicken B lymphocyte line DT40. Rad17

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The DT40 web site: sampling and connecting the genes of a B cell line

Cited by 26 publications

References 14 publications

Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium

Identification and Functional Characterization of Chicken Toll-Like Receptor 5 Reveals a Fundamental Role in the Biology of Infection with Salmonella enterica Serovar Typhimurium

Second report on chicken genes and chromosomes 2005

Critical role for chicken Rad17 and Rad9 in the cellular response to DNA damage and stalled DNA replication

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