The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation

Erikson, Oskar; Hertzberg, Magnus; Näsholm, Torgny

doi:10.1007/s11103-004-7902-9

Cited by 45 publications

(38 citation statements)

References 21 publications

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“…However, several alternative approaches are available to replace antibiotic resistance genes. Recent developments have produced new, alternative and less controversial selection systems that do not result in resistance genes in transgenic plants and require nontoxic selective chemicals, such as xylA, galT and dsdA mediating selection on xylose, galactose and D-serine, respectively, and that provide the transgenic shoots with a metabolic ascendancy over non-transgenic shoots (Erikson et al 2005;Haldrup et al 1998a). However, genes mediating selection that encode for the biosynthesis of plant growth regulators (PGRs), such as ipt, rol, pga 22, ESR1 and CKI1, give transgenic cells extra regeneration capacity with the PGRs .…”

Section: Introductionmentioning

confidence: 99%

“…Although these systems provide a convenient new selection strategy, they still rely on the performance of transgenic tissues/shoots. Moreover, most of these marker genes are bacterial in origin, and the introduction of such genes into foods raise ethical issues that may cause apprehension among the public and complicated legislation by governments (Erikson et al 2005;Leyman et al 2004). The recovery of marker-free plants without the necessity of sexual crossing is certainly an advantage; however, the diYculty in complete removal of the markers has hindered swift acceptance of these methods (Breitler et al 2004;Miki and McHugh 2004).…”

Section: Introductionmentioning

confidence: 99%

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Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation

Hsiao

Sanjaya

et al. 2006

Planta

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Gene transformation is an integral tool for plant genetic engineering. All antibiotic resistant genes currently employed are of bacterial origin and their presence in the field is undesirable. Therefore, we developed a novel and efficient plant native non-antibiotic selection system for the selection of transgenic plants in the model system Arabidopsis. This new system is based on the enhanced expression of Arabidopsis tryptophan synthase beta 1 (AtTSB1) and the use of 5-methyl-tryptophan (5MT, a tryptophan [Trp] analog) and/or CdCl2 as selection agent(s). We successfully integrated an expression cassette containing an AtT-SB1 cDNA driven by a cauliflower mosaic virus 35S promoter into Arabidopsis by floral dip transformation. Transgenic plants were efficiently selected on MS medium supplemented with 75 microM 5MT or 300 microM CdCl2 devoid of antibiotics. TSB1 selection was as efficient as the conventional hygromycin selection system. Northern blot analysis of transgenic plants selected by 5MT and CdCl2 revealed increased TSB1 mRNA transcript whereas uneven transcript levels of hygromycin phosphotransferase II (hpt) (control) was observed. Gas chromatography-mass spectrometry revealed 10-15 fold greater free Trp content in AtT-SB1 transgenic plants than in wild-type plants grown with or without 5MT or CdCl2. Taken together, the TSB1 system provides a novel selection system distinct from conventional antibiotic selection systems.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation

Hsiao

Sanjaya

et al. 2006

Planta

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“…Thus, the transgenic cells would be selected based on the differences in the toxicity of chiral amino acids and their metabolites to plants. This phenomenon was successfully demonstrated by employing dao1 and dsdA genes encoding D-amino acid oxidase and D-serine dehydratase, respectively as novel selectable markers for the identification of Arabidopsis transformants (Erikson et al 2004(Erikson et al , 2005. Ebmeier et al (2004) reported the use of ilvA gene, encoding threonine deaminase, as an efficient selectable marker in tobacco using L-O-methylthreonine as the selection agent.…”

Section: Introductionmentioning

confidence: 99%

Lysine racemase: a novel non-antibiotic selectable marker for plant transformation

et al. 2009

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A non-antibiotic based selection system using L-lysine as selection agent and the lysine racemase (lyr) as selectable marker gene for plant transformation was established in this study. L-lysine was toxic to plants, and converted by Lyr into D-lysine which would subsequently be used by the transgenic plants as nitrogen source. Transgenic tobacco and Arabidopsis plants were successfully recovered on L-lysine medium at efficiencies of 23 and 2.4%, respectively. Phenotypic characterization of transgenic plants clearly revealed the expression of normal growth and developmental characteristics as that of wild-type plants, suggesting no pleiotropic effects associated with the lyr gene. The specific activity of Lyr in transgenic tobacco plants selected on L: -lysine ranged from 0.77 to 1.06 mU/mg protein, whereas no activity was virtually detectable in the wild-type plants. In addition, the composition of the free amino acids, except aspartic acid, was not affected by the expression of the lyr gene in the transgenic tobacco plants suggesting very limited interference with endogenous amino acid metabolism. Interestingly, our findings also suggested that the plant aspartate kinases may possess an ability to distinguish the enantiomers of lysine for feedback regulation. To our knowledge, this is the first report to demonstrate that the lysine racemase selectable marker system is novel, less controversial and inexpensive than the traditional selection systems.

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“…Such selection systems were developed by Joersbo and Okkels (1996) with benzyladenine-N-3-glucuronide, Haldrup et al (1998) with D-xylose, Kunze et al (2001) with 2-deoxyglucose, Erikson et al (2004) with D-amino acids, Erikson et al (2005) with D-serine, You et al (2003) with a ferredoxin-like protein gene, or Ebmeier et al (2004) with the E. coli threonine deaminase gene as selectable markers. The phosphomannose isomerase (pmi) gene was originally used as a selectable marker by Joersbo et al (1998) for the transformation of sugar beet.…”

Section: Introductionmentioning

confidence: 99%

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Břı́za¹,

Pavingerová²,

Přikrylová³

et al. 2008

Biol. plant.

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Two selection systems for Agrobacterium tumefaciens mediated transformation of tomato and potato were compared. In the tomato (Lycopersicon esculentum cv. Moneymaker), the highest transformation rate, 4.2 %, of cotyledon explants on mannose-selection medium was obtained when mannose/sucrose concentration in the regeneration medium was 5/15 g dm -3 . The best transformation efficacy with the commonly used concentration of 100 mg dm -3 kanamycin as a selection agent was 9 %. In the potato (Solanum tuberosum cv. Bintje), the highest transformation frequency was 53.3 % when mannose concentration in the regeneration medium was 5 g dm -3 during the first 3 weeks after transformation and 10 g dm -3 afterwards. The optimum concentration of sucrose was 20 g dm -3 . The transformation efficiency using kanamycin as a selection agent at a concentration 100 mg dm -3 was 33.3 % with potato. Our results demonstrate that the transformation efficiency using mannose selection is 1.6-fold higher for potato and about 2 times lower for tomato comparing with the ordinary protocol using kanamycin.

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The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation

Cited by 45 publications

References 21 publications

Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation

Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation

Lysine racemase: a novel non-antibiotic selectable marker for plant transformation

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

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