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2018
DOI: 10.5851/kosfa.2018.e43
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The Dry-aging and Heating Effects on Protein Characteristics of Beef Longissiumus Dorsi

Abstract: The aim of this study was to investigate the effects of dry-aging (DA) and the cooking process on the myofibril protein functionalities and in vitro digestibility of proteins in beef loin. Six sirloins from beef were dry-aged for 28 d, and the control group (n=6) was analyzed 2 d postmortem for this study. Dimensional changes (reduction of thickness and surface shrinkage) after cooking were significantly greater in the control group than the DA group, whereas the shear force of the DA group was significantly l… Show more

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Cited by 16 publications
(8 citation statements)
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“…Then, the mixtures were heated at 100°C for 5 min, and separated using 10% SDS-PAGE. Next, the gel was stained with Coomassie Brilliant Blue R250 (B7920; Sigma-Aldrich, St. Louis, MO, USA), and the stained protein bands were identified by molecular weight (kDa) using a regular range protein marker (PM2510; SMOBIO Technology Inc., Hsinchu City, Taiwan) (Kim et al, 2018).…”
Section: Methodsmentioning
confidence: 99%
“…Then, the mixtures were heated at 100°C for 5 min, and separated using 10% SDS-PAGE. Next, the gel was stained with Coomassie Brilliant Blue R250 (B7920; Sigma-Aldrich, St. Louis, MO, USA), and the stained protein bands were identified by molecular weight (kDa) using a regular range protein marker (PM2510; SMOBIO Technology Inc., Hsinchu City, Taiwan) (Kim et al, 2018).…”
Section: Methodsmentioning
confidence: 99%
“…The collagen in meat was heated to dissolve during the stewing procession. The cross-linking between protein molecules is reduced, resulting in the loss of MP and the fragmentation of tissue structure, which will eventually decrease the shear force of samples ( 36 ). The shear force of the lean layer of SF-2 was the lowest.…”
Section: Resultsmentioning
confidence: 99%
“…The tenderness depends on four main components: the extent of myofibrillar protein degradation (proteolysis), sarcomere length, and the content of connective tissue ( 108 ), and endogenous proteolytic enzymes ( 109 ). Dry aging of beef Longissimus Dorsi was reported to cause an increase in myofibrillar degradation and a decrease in shear force compared to commercial beef ( 110 ). Degradation of myofibril by natural enzymes will improve meat tenderness ( 111 ).…”
Section: Biochemical Characteristics and Changes During Meat Dryingmentioning
confidence: 99%