The Drosophila wing test: A comparison of the sensitivity of different strains

Clements, Julie; Howe, Denise; Phillips, M. J.; Todd, N.K.

doi:10.1016/0165-1161(88)90026-x

Cited by 9 publications

(1 citation statement)

References 13 publications

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“…Twenty male flies (0-5 days old, after emergence), D. melanogaster, iv //rVTM3,'v", used for the radicalrelated study [ 19], were placed in test tubes (pyrex cul ture tube. 25 X 100 mm).…”

Section: Animals and Survival Studiesmentioning

confidence: 99%

Low-Level Chemiluminescence and Life Span of <i>Drosophila melanogaster</i>

Sato¹,

Miyazawa

Kobayashi³

et al. 1992

Gerontology

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Spontaneous photon emission (chemiluminescence, CL) as a monitor of free radical evolution in Drosophila melanogaster which had been maintained at 25 or 30°C for 5 days after emergence was measured. When maintained at 30°C the fly CL intensity was stronger than at 25°C. Under the condition of the higher temperature, the fly life span was shorter (mean life span = 29 days at 30°C and 63 days at 25°C), and oxygen consumption (3.7 μl/mg·h at 25°C, 4.9 μl/mg·h at 30°C) and the mobility (movement distance = 25 mm/min at 25°C, 700 mm/min at 30°C) increased, together with augmentation of phospholipid hydroperoxide in the fly total lipids. The CL spontaneously emitted from fly homogenate was decreased by the free radical scavengers both in experimtens in vivo and in vitro. The hypothesis is proposed that as the oxygen metabolism grows active, the chemiluminescent reactions that involve oxygen-dependent free radical metabolism, including membrane phospholipid hydroperoxidation, contribute to the acceleration of senescence of fly bodies.

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“…Twenty male flies (0-5 days old, after emergence), D. melanogaster, iv //rVTM3,'v", used for the radicalrelated study [ 19], were placed in test tubes (pyrex cul ture tube. 25 X 100 mm).…”

Section: Animals and Survival Studiesmentioning

confidence: 99%