The Drosophila odorant-binding protein 28a is involved in the detection of the floral odour ß-ionone

González, Daniel; Rihani, Karen; Neiers, Fabrice; Poirier, Nicolas; Fraichard, Stéphane; Gotthard, Guillaume; Chertemps, Thomas; Maı̈bèche, Martine; Ferveur, Jean‐François; Briand, Loı̈c

doi:10.1007/s00018-019-03300-4

Cited by 35 publications

(32 citation statements)

References 66 publications

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“…4c). For example, the Obp28a protein binds floral odors (Gonzalez et al 2020) and we found higher adaptive expression of Obp28a in Drosophila subgenus female abdomens. Additionally, while we haven’t detected statistically significant adaptation in males within the tested regimes, we saw a species-specific increase in D. pseudoobscura male abdomen.…”

Section: Resultsmentioning

confidence: 70%

Modeling gene expression evolution with EvoGeneX uncovers differences in evolution of species, organs and sexes

Pal

Oliver

Przytycka

2020

Preprint

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While DNA sequence evolution has been well studied, the expression of genes is also subject to evolution. Yet the evolution of gene expression is currently not well understood. In recent years, new tissue/organ specific gene expression datasets spanning several organisms across the tree of life, have become available providing the opportunity to study gene expression evolution in more detail. However, while a theoretical model to study evolution of continuous traits exist, in practice computational methods often cannot distinguish, with confidence, between alternative evolutionary scenarios. This lack of power has been attributed to the modest number of species with available expression data.To solve this challenge, we introduce EvoGeneX, a computationally efficient method to uncover the mode of gene expression evolution based on the Ornstein-Uhlenbeck process. Importantly, EvoGeneX in addition to modelling expression variations between species, models within species variation. To estimate the within species variation, EvoGeneX formally incorporates the data from biological replicates as a part of the mathematical model. We show that by modelling the within species diversity EvoGeneX significantly outperforms the currently available computational method. In addition, to facilitate comparative analysis of gene expression evolution, we introduce a new approach to measure the dynamics of evolutionary divergence of a group of genes.We used EvoGeneX to analyse the evolution of expression across different organs, species and sexes of the Drosophila genus. Our analysis revealed differences in the evolutionary dynamics of male and female gonads, and uncovered examples of adaptive evolution of genes expressed in the head and in the thorax.

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Section: Resultsmentioning

confidence: 70%

Modeling gene expression evolution with EvoGeneX uncovers differences in evolution of species, organs and sexes

Pal

Oliver

Przytycka

2020

Preprint

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“…The currently known Drosophila sex pheromones are cVA produced by males and CHCs produced by oenocytes, but males still show strong courtship for females without oenocytes (Billeter and Levine, 2015), indicating that females produce sex pheromones other than CHCs, and further research is clearly needed. β-ionone is a floral odor substance that attracts D. melanogaster and has a strong affinity with OBP28a (Gonzalez et al, 2019). Ab4 and pb2 sensilla are the most sensitive to β-ionone, and OBP28a mutants cause the responses of ab4 and pb2 sensors to β-ionone to be reduced, but they did not completely disappear, indicating that ab4 and pb2 sensors can also detect β-ionone by means other than OBP28a (Gonzalez et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…β-ionone is a floral odor substance that attracts D. melanogaster and has a strong affinity with OBP28a ( Gonzalez et al., 2019 ). Ab4 and pb2 sensilla are the most sensitive to β-ionone, and OBP28a mutants cause the responses of ab4 and pb2 sensors to β-ionone to be reduced, but they did not completely disappear, indicating that ab4 and pb2 sensors can also detect β-ionone by means other than OBP28a ( Gonzalez et al., 2019 ). Similarly, in our experiments, β-ionone bind to DsOBP69a and DsOBP76a and have the highest affinity compared to all the ligands tested.…”

Section: Discussionmentioning

confidence: 99%

Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Zhan¹,

Dewer

et al. 2021

Heliyon

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The fruit fly Drosophila suzukii is a fruit crop pest that causes a severe economic threat to soft summer fruit worldwide. The male sex pheromone, cis-vaccenyl acetate (cVA) has multiple functions in intra-species communication in Drosophila melanogaster, which is required in male to suppress male-male courtship. D. suzukii males do not produce cVA; however, the odorant receptor for cVA (Or67d) is still functional. The lack of cVA in D. suzukii casts the question of whether this pheromone might have been replaced by another compound similar to cVA that disrupts mating in D. suzukii. In order to address this question, we cloned two D. suzukii adult antenna-specific odorant-binding proteins (OBPs) DsOBP69a and DsOBP76a and aligned with their D. melanogaster orthologues. Moreover, we examined the binding properties of the newly identified recombinant proteins against 26 potential ligands including cVA, using the fluorescence-based ligand binding assay. The alignment showed that DsOBP69a and DsOBP76a, have six conserved cysteines and belong to the classic OBP family. Furthermore, our results revealed that cVA did not bind to DsOBP69a or DsOBP76a proteins. Interestingly, the floral odorant β-ionone and the bitter substance berberine chloride and coumarin displayed high binding ability. It is also worth noting that DsOBP69a and DsOBP76a have different affinities to (Z)-7-Tricosene that may reflect different functional roles. These findings suggest that DsOBP69a and DsOBP76a are potentially involved in olfaction and gustation of D. suzukii.

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“…4), unlike classic insect OBPs with six ⊍-helices. 45,46 Some OBPs can form a small seventh ⊍-helix, which enters the binding pocket to stabilize the structure when the ligand is released. 47 Interestingly, in our simulations of molecular dynamics, the seventh ⊍-helix of AmalOBP8 was quickly wileyonlinelibrary.com/journal/ps transformed into a loop structure upon binding with geranyl formate.…”

Section: Discussionmentioning

confidence: 99%

Analyses of structural dynamics revealed flexible binding mechanism for the Agrilus mali odorant binding protein 8 towards plant volatiles

Liu

2020

Pest Management Science

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BACKGROUND Volatiles from host plants are an important source of insect pest attractants and repellents. Insect odorant binding proteins (OBPs) have been widely characterized, but the molecular binding dynamics and underlying mechanisms are still not well understood. Thus, we characterized binding characteristics of AmalOBP8 from the apple buprestid beetle (Agrilus mali Matsumura), an unprecedented serious threat to rare apple germplasm resources and local ecosystems. RESULTS Fluorescence studies demonstrated that the quenching mechanism was clearly static. AmalOBP8 was found to bind with both volatiles at single independent sites. Negative thermodynamic parameters suggested that binding interactions between AmalOBP8 and both volatiles could occur spontaneously. Hydrogen bonding was the key force in AmalOBP8's binding to geranyl formate, for which the amino acid residue Trp106 played a critical role in the binding pocket. Multiple Leu residues in AmalOBP8 created a strong hydrophobic environment, and formed the binding pocket for (Z)‐3‐hexenyl hexanoate. Compared to classic OBPs, in addition to lack of one disulfide bridge, AmalOBP8 had a small α‐helix (α7) at the C‐terminus, resulting in greater flexibility and adaptability for this protein to bind with different compound molecules. CONCLUSION Key residues of AmalOBP8 in binding interactions with plant volatiles were clarified. AmalOPB8 had a large ligand binding spectrum and great flexibility in binding with plant volatiles, providing good molecular targets for screening insect attractants and repellents. Our results can promote understanding of insects' perception of various odorants, and establish a foundation for discovery of new pest control agents. © 2020 Society of Chemical Industry

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The Drosophila odorant-binding protein 28a is involved in the detection of the floral odour ß-ionone

Cited by 35 publications

References 66 publications

Modeling gene expression evolution with EvoGeneX uncovers differences in evolution of species, organs and sexes

Modeling gene expression evolution with EvoGeneX uncovers differences in evolution of species, organs and sexes

Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Analyses of structural dynamics revealed flexible binding mechanism for the Agrilus mali odorant binding protein 8 towards plant volatiles

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