The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.

Spana, Carl; Harrison, Douglas A.; Corcés, Victor G.

doi:10.1101/gad.2.11.1414

Cited by 193 publications

(141 citation statements)

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“…Circumstantial experimental evidence suggests that a specific human nuclear ZFP interacts with the Alu DNA repeats (Chesnokov et al, 1991). In Drosophilu, the dispersed gypsy retro-transposon element is specifically bound by the su (Hw) zinc finger protein (Parkhust et al, 1988); binding of su (Hw) can lead to the inactivation of nearby transcription units in a position-specific fashion (Geyer and Corces, 1992), and su (Hw) is a ubiquitous nuclear protein, localized to 100-200 sites on Drosophila polytene chromosomes (Harrison et al, 1993;Spana et al, 1988). Null mutations in the su (Hw) gene lead to female sterility correlating with deregulated chromosome aggregation and condensation (Harrison et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Sequence‐Specific Recognition of a Repetitive DNA Element by a C₂H₂ Zinc‐Finger Protein in Xenopus

Schäfer

Rausch

Bouwmeester

et al. 1994

European Journal of Biochemistry

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XFG 20-1 is one of several hundred C,H,-type zinc finger proteins of unknown biological function in Xenopus. Affinity selection of genomic DNA fragments identifies a 54-bp consensus sequence with high affinity for the XFG 20-1 protein. This sequence is part of a conserved inverted repeat in REM-1, a 0.5-kb repetitive, dispersed genomic DNA element. This finding indicates a possible link between the non-conserved nature of a large subgroup of DNA-binding C,H, zinc finger proteins and the non-conserved nature of putative genomic target sequences. The 19 zinc finger modules of XFG 20-1 are organized in three structurally distinct groups. Of these, only the first and third ones are required and are sufficient for high-affinity DNA binding. The finding that removal of five internal zinc finger units does not alter the DNase I protection pattern of the complex formed is indicative of a high degree of structural flexibilitv for linear arrays of zinc fingers, which

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Section: Discussionmentioning

confidence: 99%

Sequence‐Specific Recognition of a Repetitive DNA Element by a C₂H₂ Zinc‐Finger Protein in Xenopus

Schäfer

Rausch

Bouwmeester

et al. 1994

European Journal of Biochemistry

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“…These data suggest that the lack of enhancer blocking in flies may be caused by sequences other than the ϩ660 gypsy insulator. (19,34). Insertion into, or deletion of, binding sites within the region disrupts the insulator function (35)(36)(37), with an apparent threshold of four binding sites required for activity (38).…”

Section: Methodsmentioning

confidence: 99%

“…The function of this insulator depends on two proteins, the zinc finger DNA-binding protein, Suppressor of Hairy-wing [Su(Hw)], and the BTB͞POZ domain protein Modifier of mdg4 [Mod-(mdg4)]67.2 that is recruited to chromosomes through proteinprotein interactions with the Su(Hw) protein (19)(20)(21)(22). The gypsy insulator proteins colocalize at hundreds of genomic sites, with Ͻ1% of these sites representing positions of the gypsy retrotransposon (23).…”

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confidence: 99%

An endogenous Suppressor of Hairy-wing insulator separates regulatory domains in Drosophila

Parnell

Viering²,

Skjesol³

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…The y 2 allele is an insertion of the gypsy retrotransposon between the y gene and the y wing and body enhancers (Geyer et al 1990;Morris et al 1999a). The gypsy retrotransposon contains 12 degenerate binding sites for the Suppressor of Hairy-Wing (SuHw) protein, which are sufficient to function as a boundary element (Parkhurst et al 1988;Spana et al 1988;Geyer and Corces 1992). Both homozygous y 2 flies and y 2 /Df flies have strong yellow phenotypes in their wings and bodies.…”

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confidence: 99%

Enhancer Blocking and Transvection at the DrosophilaapterousLocus

et al. 2008

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Intra-and interchromosomal interactions have been implicated in a number of genetic phenomena in diverse organisms, suggesting that the higher-order structural organization of chromosomes in the nucleus can have a profound impact on gene regulation. In Drosophila, homologous chromosomes remain paired in somatic tissues, allowing for trans interactions between genes and regulatory elements on the two homologs. One consequence of homolog pairing is the phenomenon of transvection, in which regulatory elements on one homolog can affect the expression of a gene in trans. We report a new instance of transvection at the Drosophila apterous (ap) locus. Two different insertions of boundary elements in the ap regulatory region were identified. The boundaries are inserted between the ap wing enhancer and the ap promoter and have highly penetrant wing defects typical of mutants in ap. When crossed to an ap promoter deletion, both boundary inserts exhibit the interallelic complementation characteristic of transvection. To confirm that transvection occurs at ap, we generated a deletion of the ap wing enhancer by FRT-mediated recombination. When the wing-enhancer deletion is crossed to the ap promoter deletion, strong transvection is observed. Interestingly, the two boundary elements, which are inserted $10 kb apart, fail to block enhancer action when they are present in trans to one another. We demonstrate that this is unlikely to be due to insulator bypass. The transvection effects described here may provide insight into the role that boundary element pairing plays in enhancer blocking both in cis and in trans.

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The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.

Cited by 193 publications

References 40 publications

Sequence‐Specific Recognition of a Repetitive DNA Element by a C₂H₂ Zinc‐Finger Protein in Xenopus

Sequence‐Specific Recognition of a Repetitive DNA Element by a C₂H₂ Zinc‐Finger Protein in Xenopus

An endogenous Suppressor of Hairy-wing insulator separates regulatory domains in Drosophila

Enhancer Blocking and Transvection at the DrosophilaapterousLocus

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The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon.

Cited by 193 publications

References 40 publications

Sequence‐Specific Recognition of a Repetitive DNA Element by a C2H2 Zinc‐Finger Protein in Xenopus

Sequence‐Specific Recognition of a Repetitive DNA Element by a C2H2 Zinc‐Finger Protein in Xenopus

An endogenous Suppressor of Hairy-wing insulator separates regulatory domains in Drosophila

Enhancer Blocking and Transvection at the DrosophilaapterousLocus

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Sequence‐Specific Recognition of a Repetitive DNA Element by a C₂H₂ Zinc‐Finger Protein in Xenopus

Sequence‐Specific Recognition of a Repetitive DNA Element by a C₂H₂ Zinc‐Finger Protein in Xenopus