The dprA gene is required for natural transformation of Helicobacter pylori

Smeets, Leonard C.

doi:10.1016/s0928-8244(99)00170-4

Cited by 44 publications

(59 citation statements)

References 17 publications

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“…DprA (Smf) and SsbB (YwpH) (Berka et al, 2002;Lindner et al, 2004) are single-stranded DNA (ssDNA) binding proteins (Mortier-Barrière et al, 2007). dprA (smf) was identified in Haemophilus influenzae and H. pylori as a gene needed for transformation (Karudapuram et al, 1995;Smeets et al, 2000), and in Streptococcus pneumoniae, DprA was shown to protect incoming transforming DNA from degradation (Berge et al, 2003). YjbF (CoiA) is a protein of unknown function, needed for optimal transformation in B. subtilis (Eschevins, 2003).…”

mentioning

confidence: 99%

Multiple interactions among the competence proteins of Bacillus subtilis

Kramer¹,

Hahn²,

Dubnau³

2007

Molecular Microbiology

117

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mentioning

confidence: 99%

Multiple interactions among the competence proteins of Bacillus subtilis

Kramer¹,

Hahn²,

Dubnau³

2007

Molecular Microbiology

117

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“…DprA has an established role during transformation of many bacteria species, as mutants deficient in DprA are completely or partially deficient in transformation (Berge et al, 2003;Dwivedi et al, 2013;Karudapuram & Barcak, 1997;Mirouze et al, 2013;Mortier-Barriere et al, 2007;Quevillon-Cheruel et al, 2012;Smeets et al, 2000Smeets et al, , 2006Takata et al, 2005). B. subtilis and S. pneumoniae DprA have been extensively studied (Mortier-Barriere et al,…”

Section: Discussionmentioning

confidence: 99%

“…pylori (Smeets et al, 2000;Zhang & Blaser, 2012) and C. jejuni (Takata et al, 2005). To test the role of DprA in transformation of N. gonorrhoeae, we performed quantitative transformation assays in parental (wild-type) and dprA strains of FA1090 and MS11.…”

Section: Dpra Is Absolutely Required For Transformationmentioning

confidence: 99%

“…pylori, Bacillus subtilis and Campylobacter jejuni (Ando et al, 1999;Berge et al, 2003;Dwivedi et al, 2013;Quevillon-Cheruel et al, 2012;Smeets et al, 2000;Takata et al, 2005;Yadav et al, 2013Yadav et al, , 2014. Additionally, DprA was found to be required for transformation in N. meningitidis (Sun et al, 2005) which serves as a model for Neisseria transformation.…”

Section: Introductionmentioning

confidence: 99%

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DprA is required for natural transformation and affects pilin variation in Neisseria gonorrhoeae

Duffin

Barber

2016

Microbiology

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Natural transformation is the main means of horizontal genetic exchange in the obligate human pathogen Neisseria gonorrhoeae and drives the spread of antibiotic resistance and virulence determinants. Transformation can be divided into four steps: (1) DNA binding, (2) DNA uptake, (3) DNA processing and (4) DNA recombination into the chromosome. The DNA processing enzyme DprA has been shown to shuttle incoming ssDNA to the recombination enzyme RecA during transformation in Bacillus subtilis and Streptococcus pneumoniae. Here, we investigate the role of DprA during transformation in N. gonorrhoeae. Inactivation of dprA completely abrogated transformation of gyrB1-encoding DNA, which confers nalidixic acid resistance. The presence of the DNA uptake sequence enhances DNA uptake and transformation by binding to the minor pilus protein ComP. Loss of transformation in the dprA null mutants was independent of the DNA uptake sequence. DprA mutants exhibited increased RecA-dependent pilin variation suggesting that DprA affects pilin variation. Unlike the exquisite UV sensitivity of a recA mutant, inactivation of dprA did not affect survival following UV irradiation. These results demonstrate that DprA has a conserved function during transformation, and reveal additional effects of DprA in N. gonorrhoeae during pilin variation.

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“…Furthermore, ComB9 was identified as a periplasmic protein attached to the membrane, and the N-terminus of ComB10 seems to be anchored in the cytoplasmic membrane, while the main part of the protein is located in the periplasm [42]. Apart from the ComB system, additional proteins involved at different steps in the transformation competence process have also been identified, such as the cytoplasmatic proteins RecA [43], DprA [44], the secreted protein ComH [45], and the cytoplasmatic channel, ComEC, previously described in Bacillus subtilis [46]. These factors also appear to be essential for DNA uptake in H. pylori.…”

Section: General Role Of Type IV Secretion Systems (T4sss) In Dna Excmentioning

confidence: 99%

DNA transfer in the gastric pathogen Helicobacter pylori

Fernández-González

Backert

2014

J Gastroenterol

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The gastric pathogen Helicobacter pylori is one of the most genetically diverse bacteria. Recombination and DNA transfer contribute to its genetic variability and enhance host adaptation. Among the strategies described to increase genetic diversity in bacteria, DNA transfer by conjugation is one of the best characterized. Using this mechanism, a fragment of DNA from a donor cell can be transferred to a recipient, always mediated by a conjugative nucleoprotein complex, which is evolutionarily related to type IV secretion systems (T4SSs). Interestingly, the H. pylori chromosomes can encode up to four T4SSs, including the cagPAI, comB, tfs3, and tfs4 genes, some of which are known to promote chronic H. pylori infection. The T4SS encoded by the cagPAI mediates the injection of the effector protein CagA and proinflammatory signaling, and the comB system is involved in DNA uptake from the environment. However, the role of tfs3 and tfs4 is not yet clear. The presence of a functional XerD tyrosine recombinase and 5 0 AAAGAATG-3 0 border sequences as well as two putative conjugative relaxases (Rlx1 and Rlx2), a coupling protein (TraG), and a chromosomal region carrying a putative origin of transfer (oriT) suggest the existence of a DNA transfer apparatus in tfs4. Moreover, a conjugation-like DNA transfer mechanism in H. pylori has already been described in vitro, but whether this occurs in vivo is still unknown. Some extrachromosomal plasmids and phages are also present in various H. pylori strains. Genetic exchange among plasmids and chromosomes, and involved DNA mobilization events, could explain part of H. pylori's genetic diversity. Here, we review our knowledge about the possible DNA transfer mechanisms in H. pylori and its implications in bacterial adaptation to the host environment.

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The dprA gene is required for natural transformation of Helicobacter pylori

Cited by 44 publications

References 17 publications

Multiple interactions among the competence proteins of Bacillus subtilis

Multiple interactions among the competence proteins of Bacillus subtilis

DprA is required for natural transformation and affects pilin variation in Neisseria gonorrhoeae

DNA transfer in the gastric pathogen Helicobacter pylori

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