The dominant negative ARM domain uncovers multiple functions of PUB13 in Arabidopsis immunity, flowering, and senescence

Zhou, Jianguang; Lu, Dongping; Xu, Guangyuan; Finlayson, Scott A.; He, Ping; Shan, Libo

doi:10.1093/jxb/erv148

Cited by 79 publications

(72 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, ubiquitination regulation of leaf senescence has attracted increasing attention (Kim et al ., ). The U‐box E3 ligases, PUB12 and PUB13, as well as the RING E3 ligase, ATL31, have been found to function as repressors during leaf senescence (Aoyama et al ., ; Zhou et al ., ). In this study, we found that, in addition to the gene expression of MdbHLH93 (Fig.…”

Section: Discussionmentioning

confidence: 97%

MdbHLH93, an apple activator regulating leaf senescence, is regulated by ABA and MdBT2 in antagonistic ways

Zhang

et al. 2019

New Phytologist

View full text Add to dashboard Cite

Summary The molecular mechanism of leaf senescence in apple (Malus domestica) is still not fully understood. We used gene expression analysis and protein–protein interactions to decipher the relationships of abscisic acid (ABA) and two proteins, MdbHLH93 and MdBT2, in the senescence process. We found that MdbHLH93 promoted leaf senescence and the expression of senescence‐related genes, which exhibited similar effects to ABA on leaf senescence. MdbHLH93 activated directly the transcription of MdSAG18. We also found that an ABA‐responsive protein, MdBT2, interacted directly with MdbHLH93, and induced the ubiquitination and degradation of the MdbHLH93 protein, and thus delayed leaf senescence. Our findings provide new insights into the regulatory network of leaf senescence through the functional interactions among ABA, MdbHLH93 and MdBT2.

show abstract

Section: Discussionmentioning

confidence: 97%

MdbHLH93, an apple activator regulating leaf senescence, is regulated by ABA and MdBT2 in antagonistic ways

Zhang

et al. 2019

New Phytologist

View full text Add to dashboard Cite

show abstract

“…However, rice ATPase XB24 promotes XA21 autophosphorylation via its ATPase activity but inhibits XA21-mediated resistance (21). The Arabidopsis E3 ubiquitin ligases PUB12 and PUB13 are recruited to FLS2 in a BAK1-dependent manner and directly ubiquitinate FLS2 for FLS2 degradation, leading to the attenuation of FLS2 signaling (99, 175). Ubiquitination of membrane receptors also leads to receptor endocytosis and lysosome/vacuole targeting.…”

Section: Pattern Recognition Receptor Complexes Perceiving Patternsmentioning

confidence: 99%

From Chaos to Harmony: Responses and Signaling upon Microbial Pattern Recognition

Xiao

Feng

et al. 2017

Annu. Rev. Phytopathol.

Self Cite

418

406

View full text Add to dashboard Cite

Pathogen- or microbe-associated molecular patterns (PAMPs/MAMPs) are detected as nonself by host pattern recognition receptors (PRRs) and activate pattern-triggered immunity (PTI). Microbial invasions often trigger the production of host-derived endogenous signals referred to as danger-or damage-associated molecular patterns (DAMPs), which are also perceived by PRRs to modulate PTI responses. Collectively, PTI contributes to host defense against infections by a broad range of pathogens. Remarkable progress has been made toward demonstrating the cellular and physiological responses upon pattern recognition, elucidating the molecular, biochemical, and genetic mechanisms of PRR activation, and dissecting the complex signaling networks that orchestrate PTI responses. In this review, we present an update on the current understanding of how plants recognize and respond to nonself patterns, a process from which the seemingly chaotic responses form into a harmonic defense.

show abstract

“…Yeast two-hybrid (Y2H) and Co-IP experiments using the BAK1 kinase domain as a bait demonstrated that flg22 treatment stimulates interaction between BAK1 and the plant U box (PUB) E3 ubiquitin ligases PUB12 and PUB13, and that the BAK1-PUB12/13 complex associates with FLS2 within 30 seconds of flg22 treatment [12]. Kinase assays using protoplasts expressing BAK1 or a kinase-inactive mutant indicated that BAK1 phosphorylates the C-terminal Armadillo (ARM) repeat domain of PUB13 in response to flg22 treatment, and chemical inhibition of kinase activity confirmed that BAK1-dependent phosphorylation is required for flg22-induced FLS2-PUB12/PUB13 interaction [12,13]. In vitro ubiquitination assays confirmed that PUB12 and PUB13 polyubiquitinate the cytosolic domain of FLS2, but not BAK1 or BIK1 [12].…”

Section: Ubiquitination and N-glycosylation Contribute To Pti Throughmentioning

confidence: 99%

Post-translational regulation of plant immunity

Withers

Dong

2017

Current Opinion in Plant Biology

131

View full text Add to dashboard Cite

The dominant negative ARM domain uncovers multiple functions of PUB13 in Arabidopsis immunity, flowering, and senescence

Abstract: HighlightEctopic expression of the PUB13 ARM domain in planta mimics the pub13 mutant with enhanced immune responses, stress-induced leaf senescence, and early flowering.

Cited by 79 publications

References 51 publications

MdbHLH93, an apple activator regulating leaf senescence, is regulated by ABA and MdBT2 in antagonistic ways

MdbHLH93, an apple activator regulating leaf senescence, is regulated by ABA and MdBT2 in antagonistic ways

From Chaos to Harmony: Responses and Signaling upon Microbial Pattern Recognition

Post-translational regulation of plant immunity

Contact Info

Product

Resources

About