The DOF Transcription Factor SlDOF10 Regulates Vascular Tissue Formation During Ovary Development in Tomato

Rojas-Gracia, Pilar; Roque, Edelín; Medina, Mónica; López-Martín, María Jesús; Cañas, Luis A.; Beltrán, José Pío; Gómez‐Mena, Concepción

doi:10.3389/fpls.2019.00216

Cited by 23 publications

(15 citation statements)

References 47 publications

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“… Wen et al (2016) found that CsDof TF might play a role against biotic stresses in cucumber ( Cucumis sativus ). Rojas-Gracia et al (2019) stated the SIDOF10 TF involved regulation of vascular tissue in tomatoes ( Solanum lycopersicum ). C2H2 TF regulates abiotic stress responses in plants ( Han et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

Rizwan

Shaozhong

et al. 2022

Front. Plant Sci.

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Plant and fruit surfaces are covered with cuticle wax and provide a protective barrier against biotic and abiotic stresses. Cuticle wax consists of very-long-chain fatty acids (VLCFAs) and their derivatives. β-Ketoacyl-CoA synthase (KCS) is a key enzyme in the synthesis of VLCFAs and provides a precursor for the synthesis of cuticle wax, but the KCS gene family was yet to be reported in the passion fruit (Passiflora edulis). In this study, thirty-two KCS genes were identified in the passion fruit genome and phylogenetically grouped as KCS1-like, FAE1-like, FDH-like, and CER6-like. Furthermore, thirty-one PeKCS genes were positioned on seven chromosomes, while one PeKCS was localized to the unassembled genomic scaffold. The cis-element analysis provides insight into the possible role of PeKCS genes in phytohormones and stress responses. Syntenic analysis revealed that gene duplication played a crucial role in the expansion of the PeKCS gene family and underwent a strong purifying selection. All PeKCS proteins shared similar 3D structures, and a protein–protein interaction network was predicted with known Arabidopsis proteins. There were twenty putative ped-miRNAs which were also predicted that belong to nine families targeting thirteen PeKCS genes. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation results were highly associated with fatty acid synthase and elongase activity, lipid metabolism, stress responses, and plant-pathogen interaction. The highly enriched transcription factors (TFs) including ERF, MYB, Dof, C2H2, TCP, LBD, NAC, and bHLH were predicted in PeKCS genes. qRT-PCR expression analysis revealed that most PeKCS genes were highly upregulated in leaves including PeKCS2, PeKCS4, PeKCS8, PeKCS13, and PeKCS9 but not in stem and roots tissues under drought stress conditions compared with controls. Notably, most PeKCS genes were upregulated at 9th dpi under Fusarium kyushuense biotic stress condition compared to controls. This study provides a basis for further understanding the functions of KCS genes, improving wax and VLCFA biosynthesis, and improvement of passion fruit resistance.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

Rizwan

Shaozhong

et al. 2022

Front. Plant Sci.

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“…The expression of the AtDOF2.4/PEAR1 (PHLOEM EARLY DOF1), AtDOF5.1/PEAR2 (PHLOEM EARLY DOF2), AtDOF3.2/DOF6, AtDOF5.3/TMO6, AtDOF1.1/OBP2 (OBF BINDING PROTEIN2) and AtDOF5.6/HCA2 (HIGH CAMBIAL ACTIVITY2) genes was reported to be regulated by auxin and cytokinin in the sieve tube of the primary phloem (Miyashima et al, 2019;Smetana et al, 2019). Downregulation of tomato SlDOF10 triggered parthenocarpic development while suppressing vascular tissue development (Rojas-Gracia et al, 2019). MdDOF15, MdDOF18, and MdDOF48 were significantly upregulated in apple during pollen tube growth and development (Yang et al, 2018b).…”

Section: Functions Of Dofs In Plant Growth and Developmentmentioning

confidence: 99%

DOF transcription factors: Specific regulators of plant biological processes

Zou

Sun

2023

Front. Plant Sci.

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Plant biological processes, such as growth and metabolism, hormone signal transduction, and stress responses, are affected by gene transcriptional regulation. As gene expression regulators, transcription factors activate or inhibit target gene transcription by directly binding to downstream promoter elements. DOF (DNA binding with One Finger) is a classic transcription factor family exclusive to plants that is characterized by its single zinc finger structure. With breakthroughs in taxonomic studies of different species in recent years, many DOF members have been reported to play vital roles throughout the plant life cycle. They are not only involved in regulating hormone signals and various biotic or abiotic stress responses but are also reported to regulate many plant biological processes, such as dormancy, tissue differentiation, carbon and nitrogen assimilation, and carbohydrate metabolism. Nevertheless, some outstanding issues remain. This article mainly reviews the origin and evolution, protein structure, and functions of DOF members reported in studies published in many fields to clarify the direction for future research on DOF transcription factors.

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“…In addition, these male sterile plants could be used in hybrid breeding applications as very convenient parental lines. The transgenic lines generated could also be useful tools to investigate the molecular mechanisms accountable for the observed metabolic phenotypes, and also to understand the connection between impaired anther development and parthenocarpy (Medina et al, 2013; Rojas-Gracia et al, 2017, 2019).…”

Section: Side Effects In the Psend1::barnase-barstar Male Sterile Plamentioning

confidence: 99%

Engineered Male Sterility by Early Anther Ablation Using the Pea Anther-Specific Promoter PsEND1

et al. 2019

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Genetic engineered male sterility has different applications, ranging from hybrid seed production to bioconfinement of transgenes in genetic modified crops. The impact of this technology is currently patent in a wide range of crops, including legumes, which has helped to deal with the challenges of global food security. Production of engineered male sterile plants by expression of a ribonuclease gene under the control of an anther- or pollen-specific promoter has proven to be an efficient way to generate pollen-free elite cultivars. In the last years, we have been studying the genetic control of flower development in legumes and several genes that are specifically expressed in a determinate floral organ were identified. Pisum sativum ENDOTHECIUM 1 ( PsEND1 ) is a pea anther-specific gene displaying very early expression in the anther primordium cells. This expression pattern has been assessed in both model plants and crops (tomato, tobacco, oilseed rape, rice, wheat) using genetic constructs carrying the PsEND1 promoter fused to the uidA reporter gene. This promoter fused to the barnase gene produces full anther ablation at early developmental stages, preventing the production of mature pollen grains in all plant species tested. Additional effects produced by the early anther ablation in the PsEND1 :: barnase-barstar plants, with interesting biotechnological applications, have also been described, such as redirection of resources to increase vegetative growth, reduction of the need for deadheading to extend the flowering period, or elimination of pollen allergens in ornamental plants ( Kalanchoe, Pelargonium ). Moreover, early anther ablation in transgenic PsEND1::barnase-barstar tomato plants promotes the developing of the ovaries into parthenocarpic fruits due to the absence of signals generated during the fertilization process and can be considered an efficient tool to promote fruit set and to produce seedless fruits. In legumes, the production of new hybrid cultivars will contribute to enhance yield and productivity by exploiting the hybrid vigor generated. The PsEND1::barnase-barstar construct could be also useful to generate parental lines in hybrid breeding approaches to produce new cultivars in different legume species.

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The DOF Transcription Factor SlDOF10 Regulates Vascular Tissue Formation During Ovary Development in Tomato

Cited by 23 publications

References 47 publications

Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

DOF transcription factors: Specific regulators of plant biological processes

Engineered Male Sterility by Early Anther Ablation Using the Pea Anther-Specific Promoter PsEND1

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