The diversity of plant carbohydrate hydrolysis in nature and technology

Buckeridge, Marcos Silveira

doi:10.1016/b978-0-323-99986-1.00015-6

Cited by 1 publication

(1 citation statement)

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“…Instead, based on Phytozome and STRING database, it is responsible for gibberellin-regulated protein and protein interaction with xyloglucan endotransglucosylase/hydrolase (Table 4). Xyloglucan endotransglucosylase/hydrolase catalyzes the cleavage of the main chain of xyloglucan molecules until only glucose, xylose, and galactose are produced, of which glucose is an essential component of the sucrose metabolism (Buckeridge, 2023). Two candidate genes, Glyma.05g025300 and Glyma.13g077900, showed high total gene expression levels for stachyose content in developing seed tissues and total expression levels across 19 different parts of the soybean plant (Figures 5C, D).…”

Section: Discussionmentioning

confidence: 99%

Novel genetic resources associated with sucrose and stachyose content through genome-wide association study in soybean (Glycine max (L.) Merr.)

Lee,

Lara,

Moseley

et al. 2023

Front. Plant Sci.

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The nutritional value of soybean [Glycine max (L.) Merr.] for animals is influenced by soluble carbohydrates, such as sucrose and stachyose. Although sucrose is nutritionally desirable, stachyose is an antinutrient causing diarrhea and flatulence in non-ruminant animals. We conducted a genome-wide association study of 220 soybean accessions using 21,317 single nucleotide polymorphisms (SNPs) from the SoySNP50K iSelect Beadchip data to identify significant SNPs associated with sucrose and stachyose content. Seven significant SNPs were identified for sucrose content across chromosomes (Chrs.) 2, 8, 12, 17, and 20, while thirteen significant SNPs were identified for stachyose content across Chrs. 2, 5, 8, 9, 10, 13, 14, and 15. Among those significant SNPs, three sucrose-related SNPs on Chrs. 8 and 17 were novel, while twelve stachyose-related SNPs on Chrs. 2, 5, 8, 9, 10, 13, 14, and 15 were novel. Based on Phytozome, STRING, and GO annotation, 17 and 24 candidate genes for sucrose and stachyose content, respectively, were highly associated with the carbohydrate metabolic pathway. Among these, the publicly available RNA-seq Atlas database highlighted four candidate genes associated with sucrose (Glyma.08g361200 and Glyma.17g258100) and stachyose (Glyma.05g025300 and Glyma.13g077900) content, which had higher gene expression levels in developing seed and multiple parts of the soybean plant. The results of this study will extend knowledge of the molecular mechanism and genetic basis underlying sucrose and stachyose content in soybean seed. Furthermore, the novel candidate genes and SNPs can be valuable genetic resources that soybean breeders may utilize to modify carbohydrate profiles for animal and human usage.

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Section: Discussionmentioning

confidence: 99%