The diversity of conjugative relaxases and its application in plasmid classification

Garcillán-Barcia, M. Pilar; Francia, María Victoria; Cruz, Fernando de la

doi:10.1111/j.1574-6976.2009.00168.x

Cited by 468 publications

(697 citation statements)

References 146 publications

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“…pSmeSM11b of S. meliloti strain SM11 (Stiens et al, 2007). The predicted origin of conjugal transfer (oriT) of pSinA is placed between the traC and traA genes, and its location and nucleotide sequence (5'-CCAAGGGCGCAATTATACGTCGCTGACGCGACGTT-3') are conserved among plasmids belonging to the MOB Q2 family (Garcillán-Barcia et al, 2009). …”

Section: The Plasmid Backbonementioning

confidence: 99%

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Drewniak

Dziewit

Ciezkowska

et al. 2013

Journal of Biotechnology

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Plasmid pSinA of Sinorhizobium sp. M14 (Alphaproteobacteria) is the first described, natural, self-transferable plasmid harboring a complete set of genes for oxidation of arsenite. Removal of this plasmid from cells of the host strain caused the loss of resistance to arsenic and heavy metals (Cd, Co, Zn and Hg) and abolished the ability to grow on minimal salt medium supplemented with sodium arsenite as the sole energy source. Plasmid pSinA was introduced into other representatives of Alphaproteobacteria which resulted in acquisition of new abilities concerning arsenic resistance and oxidation, as well as heavy metals resistance. Microcosm experiments revealed that plasmid pSinA can also be transferred via conjugation into other indigenous bacteria from microbial community of As-contaminated soils, including representatives of Alpha-and Gammaproteobacteria. Analysis of "natural" transconjugants showed that pSinA is functional (expresses arsenite oxidase) and is stably maintained in their cells after approximately 60 generations of growth under nonselective conditions. This work clearly demonstrates that pSinA is a self-transferable, broad-host-range plasmid, which plays an important role in horizontal transfer of arsenic metabolism genes.

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Section: The Plasmid Backbonementioning

confidence: 99%

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Drewniak

Dziewit

Ciezkowska

et al. 2013

Journal of Biotechnology

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“…A classification scheme has been proposed for fully sequenced plasmids based on phylogenetic analysis of the mobilization regions. Garcillan-Barcia et al, 2009;Smet et al, 2010;Smillie et al, 2010;Woodford et al, 2009) Characterization of plasmids on which ESBL and/or AmpC genes are located is an essential tool to study the epidemiology of these genes and plasmids. Since in Enterobacteriaceae often several different plasmids are present in each isolate, a structured approach is needed to identify the characteristics of the plasmid on which the β-lactamase genes are located.…”

Section: Whole Plasmid Sequencingmentioning

confidence: 99%

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

2011

EFSA Journal

240

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The potential contribution of food-producing animals or foods to public health risks by ESBL and/or AmpCproducing bacteria is related to specific plasmid-mediated ESBL and/or AmpC genes encoded by a number of organisms. The predominant ESBL families encountered are CTX-M, TEM, and SHV; the predominant AmpCfamily is CMY. The most common genes associated with this resistance in animals are bla CTX-M-1 (the most commonly identified ESBL), and bla CTX-M-14 , followed by bla and bla Among the genes encoding AmpC-type β-lactamases, bla CMY-2 is the most common.The bacterial species most commonly identified with these genes are Escherichia coli and non-typhoidal Salmonella. ESBL/AmpC transmission is mainly driven by integrons, insertion sequences, transposons and plasmids, some of which are homologous in isolates from both food-production animals and humans. Cefotaxime is used as the drug of choice for optimum detection of bla ESBL and/or bla AmpC genes. The preferred method for isolation of ESBL-and/or AmpC-producers is screening on selective agar preceded by selective enrichment in a broth.The establishment of risk factors for occurrence of ESBL/AmpC-producing bacteria is particularly complicated by the data unavailability or lack of its accuracy. The use of antimicrobials is a risk factor for the selection and spread of resistant clones, resistance genes and plasmids. Since most ESBL-and AmpC-producing strains carry additional resistances to other commonly-used veterinary drugs, generic antimicrobial use is a risk factor for ESBL/AmpC and it is not restricted specifically to the use of cephalosporins. An additional risk factor is extensive trade of animals in EU MS. There are no data on the comparative efficiency of individual control options in reducing public health risks caused by ESBL and/or AmpC-producing bacteria related to food-producing animals. Prioritisation is complex, but it is considered that a highly effective control option would be to stop all uses of cephalosporins/systemically active 3 rd /4 th generation cephalosporins, or to restrict their use (use only allowed under specific circumstances). As co-resistance is an important issue, it is also of high priority to decrease the total antimicrobial use in animal production in the EU. KEY WORDSResistance, ESBLs, AmpC, occurrence, transmission, control options, public health microbiology. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum beta (β)-lactamases (ESBL) and/or AmpC β-lactamases (AmpC) in food and foodproducing animals. In particular, the Panel was asked: (i) to propose a definition of the ESBL-and/or AmpC-producing bacterial strains and genes relevant for public health and linked to food-producing animals or food borne transmission; (ii) to review the information on the epidemiology of acquired resistance to broad spectrum cephalosporins including the genes coding for ...

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“…Horizontal transmission of ICEs starts by their excision from the host chromosome through site-specific recombination and subsequent processing of the excised circularized DNA for conjugation, similar to conjugative plasmids (20,21) (Fig. 1A).…”

mentioning

confidence: 99%

Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element

Delavat

Mitri

Pelet

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

106

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Horizontal gene transfer is an important evolutionary mechanism for bacterial adaptation. However, given the typical low transfer frequencies in a bacterial population, little is known about the fate and interplay of donor cells and the mobilized DNA during transfer. Here we study transfer of an integrative and conjugative element (ICE) among individual live bacterial cells. ICEs are widely distributed mobile DNA elements that are different than plasmids because they reside silent in the host chromosome and are maintained through vertical descent. Occasionally, ICEs become active, excise, and transmit their DNA to a new recipient, where it is reintegrated. We develop a fluorescent tool to differentiate excision, transfer, and reintegration of a model ICE named ICEclc (for carrying the clc genes for chlorocatechol metabolism) among single Pseudomonas cells by using timelapse microscopy. We find that ICEclc activation is initiated in stationary phase cells, but excision and transfer predominantly occur only when such cells have been presented with new nutrients. Donors with activated ICE develop a number of different states, characterized by reduced cell division rates or growth arrest, persistence, or lysis, concomitant with ICE excision, and likely, ICE loss or replication. The donor cell state transitions can be described by using a stochastic model, which predicts that ICE fitness is optimal at low initiation rates in stationary phase. Despite highly variable donor cell fates, ICE transfer is remarkably robust overall, with 75% success after excision. Our results help to better understand ICE behavior and shed a new light on bacterial cellular differentiation during horizontal gene transfer.

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The diversity of conjugative relaxases and its application in plasmid classification

Cited by 468 publications

References 146 publications

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element

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