The diversity and potential function of endophytic bacteria isolated from Kobreasia capillifolia at alpine grasslands on the Tibetan Plateau, China

Wang, Ying; Yang, Chengde; Yao, Yonghui; Wang, Yuqin; Zhang, Zhenfen; Xue, Li

doi:10.1016/s2095-3119(15)61248-5

Cited by 19 publications

(9 citation statements)

References 41 publications

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“…Finally, the bacteria, which could produce siderophore, were screened for N 2 fixation [ 40 ], P solubilization [ 41 ], and K solubilization [ 34 ] on Ashby’s, Pikovaskaia’s (PKO; Ca 3 (PO 4 ) 2 ), and Aleksandrov (potassium feldspar) media with an Oxford cup, respectively. Then, 10 μL of bacterial suspension were inoculated into the Oxford cup of three different media at 30 °C for 24 h; then, the sizes of the colonies and the halos were measured.…”

Section: Methodsmentioning

confidence: 99%

Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Liu

Chen

et al. 2021

Plants

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Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

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Section: Methodsmentioning

confidence: 99%

Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Liu

Chen

et al. 2021

Plants

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“…Many studies have reported that strains belonging to the same genera identified in our study confer several beneficial traits to their host plants, including enhanced resistance against certain plant pathogens and/or growth promotion (Araújo et al, 2002;Berg & Hallmann, 2006;Khan et al, 2014;Rashid, Charles & Glick, 2012;Rout & Chrzanowski, 2009;Sessitsch, Reiter & Berg, 2004;Ying et al, 2016). We assessed the antagonistic activity of selected isolates of bacterial species against L. maculans (the causal agent of phoma stem canker in oilseed rape) through dual culture bioassays and observed that Methylobacterium fujisawaense and Methylobacterium phyllosphaerae possessed antagonistic potential against the pathogen.…”

Section: Discussionmentioning

confidence: 83%

Methylobacterium, a major component of the culturable bacterial endophyte community of wild Brassica seed

Roodi

Millner

McGill

et al. 2020

PeerJ

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Background Plants are commonly colonized by a wide diversity of microbial species and the relationships created can range from mutualistic through to parasitic. Microorganisms that typically form symptomless associations with internal plant tissues are termed endophytes. Endophytes associate with most plant species found in natural and managed ecosystems. They are extremely important plant partners that provide improved stress tolerance to the host compared with plants that lack this symbiosis. Plant domestication has reduced endophyte diversity and therefore the wild relatives of many crop species remain untapped reservoirs of beneficial microbes. Brassica species display immense diversity and consequently provide the greatest assortment of products used by humans from a single plant genus important for agriculture, horticulture, bioremediation, medicine, soil conditioners, composting crops, and in the production of edible and industrial oils. Many endophytes are horizontally transmitted, but some can colonize the plant’s reproductive tissues, and this gives these symbionts an efficient mechanism of propagation via plant seed (termed vertical transmission). Methods This study surveyed 83 wild and landrace Brassica accessions composed of 14 different species with a worldwide distribution for seed-originating bacterial endophytes. Seed was stringently disinfected, sown within sterile tissue culture pots within a sterile environment and incubated. After approximately 1-month, direct isolation techniques were used to recover bacterial endophytes from roots and shoots of symptomless plants. Bacteria were identified based on the PCR amplification of partial 16S rDNA gene sequences and annotated using the BLASTn program against the NCBI rRNA database. A diversity index was used as a quantitative measure to reflect how many different bacterial species there were in the seed-originating microbial community of the Brassica accessions sampled. Results Bacterial endophytes were recovered from the majority of the Brassica accessions screened. 16S rDNA gene sequencing identified 19 different bacterial species belonging to three phyla, namely Actinobacteria, Firmicutes and Proteobacteria with the most frequently isolated species being Methylobacterium fujisawaense, Stenotrophomonas rhizophila and Pseudomonas lactis. Methylobacterium was the dominant genus composing 56% of the culturable isolated bacterial community and was common in 77% of accessions possessing culturable bacterial endophytes. Two selected isolates of Methylobacterium significantly promoted plant growth when inoculated into a cultivar of oilseed rape and inhibited the growth of the pathogen Leptosphaeria maculans in dual culture. This is the first report that investigates the seed-originating endophytic microorganisms of wild Brassica species and highlights the Brassica microbiome as a resource for plant growth promoting bacteria and biological control agents.

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“…In the current study, a variety of potential rhizobia were isolated from nodules from legumes in the low-elevation Qira Desert-Oasis Transition Zone, and a few patterns suggested host preference: Rhizobium lemnae relatives from Albizia julibrissin and Ensifer kummerowiae relatives from Alhagi sparsifolia . A previous study of root nodule endophytes within several Albizia species and colocalized Acacia and Leuceana species in China uncovered patterns of host preference, with Bradyrhizobium , Mesorhizobium , and Rhizobium strains being isolated from Albizia ( Wang et al, 2016 ). Thus, the isolation of a distinct clade of Rhizobium from Albizia in this study is not unexpected, although the isolation of a few strains of Bosea , Devosia , and Ensifer from Albizia may be unique.…”

Section: Discussionmentioning

confidence: 97%

“…P. brassicacearum is a well-known plant growth-promoting endophyte within the Pseudomonas fluorescens group ( Gislason and de Kievit, 2020 ), but the characterized strains of P. brassicacearum do grow at 4°C, so soil temperature may not be a direct driver of its distribution. A study of wild legumes in high-elevation grasslands on the Tibetan Plateau (∼2,900 m) also failed to recover Pseudomonas endophytes, and the authors similarly suggested that this ecosystem does not favor Pseudomonas endophytes ( Wang et al, 2016 ). In contrast, in a separate study of alpine mosses in the Qilian Mountains in the Qinghai-Tibet Plateau, Pseudomonas strains accounted for over half of the isolates ( Lan et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China

et al. 2021

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Bacteria in root nodules of legumes play important roles in promoting plant growth. In this study, we investigated root nodule-associated bacteria isolated from leguminous plants along an elevation gradient on the northern slope of the Kunlun Mountains, China, using a cultivation approach. In total, 300 isolates were obtained from seven legume species within six ecological zones. Isolates were identified based on 16S rRNA gene phylogenetic analysis and potential rhizobia were further identified using a recA gene phylogeny. Among the isolates, Bacillales (particularly Bacillus) were the dominant isolates from all host legumes and all elevations (63.5%), followed by Rhizobiales (13%) and Pseudomonadales (11.7%). Less than 3% of the isolates belonged to Burkholderiales, Paenibacillales, Enterobacteriales, Actinomycetales, Sphingomonadales, Xanthomonadales, Chitinophagales, Brevibacillales, Staphylococcales, or Mycobacteriales. A few elevation-specific patterns emerged within the Bacillales and Pseudomonadales. For example, isolates related to the psychrotroph Bacillus psychrosaccharolyticus were only isolated from the highest elevation sites (>3,500 m) whereas those related to the mesophile Bacillus endophyticus were only isolated from lowest elevation sites (1,350 m), suggestive of a role of soil temperature in their distribution. Similarly, isolates related to Pseudomonas brassicacearum were the dominant Pseudomonadales isolates, but they were only isolated from middle and low elevations (<3,200 m). A total of 39 isolates belonged to the Rhizobiales, 36 of which were confirmed to the genus level using the recA gene. In all, Rhizobiales isolates were obtained from five different host legumes spanning the entire elevation gradient. Those from the low-elevation Qira Desert-Oasis Transition Zone (1,350–1,960 m) suggested some patterns of host preference. For example, most isolates from Albizia julibrissin formed a monophyletic group related to Rhizobium lemnae and most from Alhagi sparsifolia were closely related to Ensifer kummerowiae. In general, this study shows that most bacteria associated with root nodules of legumes are widely distributed in distinct ecological zones within a single geographic region but suggests that both climate and host interactions may influence their distributions.

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The diversity and potential function of endophytic bacteria isolated from Kobreasia capillifolia at alpine grasslands on the Tibetan Plateau, China

Cited by 19 publications

References 41 publications

Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Methylobacterium, a major component of the culturable bacterial endophyte community of wild Brassica seed

Diversity of Root Nodule-Associated Bacteria of Diverse Legumes Along an Elevation Gradient in the Kunlun Mountains, China

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