The Diversity and Functional Capacity of Microbes Associated with Coastal Macrophytes

Miranda, Khashiff K.; Weigel, Brooke L.; Fogarty, Emily C.; Veseli, Iva; Giblin, Anne E.; Eren, A. Murat; Pfister, Catherine A.

doi:10.1128/msystems.00592-22

Cited by 11 publications

(19 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found a small number of ASVs on kelp in the nursery that persisted after outplanting, despite large overall shifts in kelp microbiome composition. The ASVs from the nursery found on outplanted kelp, including members of Rhodobacteraceae and Saprospiraceae, are common in kelp, macroalgal, and microalgal microbiomes (Kimbrel et al, 2019;Lemay et al, 2020Lemay et al, , 2021Miranda et al, 2022;Weigel & Pfister, 2019). They are likely well adapted to hostassociated niches (e.g., metabolizing host-derived compounds; Florez et al, 2017, Schultz-Johansen et al, 2018 and may produce antimicrobial compounds (Wiese et al, 2009) that shape microbiome composition.…”

Section: Discussionmentioning

confidence: 99%

Successional dynamics of the cultivated kelp microbiome

Davis

Zeinert

Byrne

et al. 2023

Journal of Phycology

View full text Add to dashboard Cite

Kelp are important primary producers that are colonized by diverse microbes that can have both positive and negative effects on their hosts. The kelp microbiome could support the burgeoning kelp cultivation sector by improving host growth, stress tolerance, and resistance to disease. Fundamental questions about the cultivated kelp microbiome still need to be addressed before microbiome‐based approaches can be developed. A critical knowledge gap is how cultivated kelp microbiomes change as hosts grow, particularly following outplanting to sites that vary in abiotic conditions and microbial source pools. In this study we assessed if microbes that colonize kelp in the nursery stage persist after outplanting. We characterized microbiome succession over time on two species of kelp, Alaria marginata and Saccharina latissima, outplanted to open ocean cultivation sites in multiple geographic locations. We tested for host‐species specificity of the microbiome and the effect of different abiotic conditions and microbial source pools on kelp microbiome stability during the cultivation process. We found the microbiome of kelp in the nursery is distinct from that of outplanted kelp. Few bacteria persisted on kelp following outplanting. Instead, we identified significant microbiome differences correlated with host species and microbial source pools at each cultivation site. Microbiome variation related to sampling month also indicates that seasonality in host and/or abiotic factors may influence temporal succession and microbiome turnover in cultivated kelps. This study provides a baseline understanding of microbiome dynamics during kelp cultivation and highlights research needs for applying microbiome manipulation to kelp cultivation.

show abstract

Section: Discussionmentioning

confidence: 99%

Successional dynamics of the cultivated kelp microbiome

Davis

Zeinert

Byrne

et al. 2023

Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…The data in this study are in direct alignment with existing marine biology studies such as that on Chlorella ( Chlorella vulgaris ), Spirulina, Dulse ( Palmaria palmate ), Irish Moss ( Chondrus crispus ), Seamoss (combination of Irish Moss and Bladderwrack ( Fucus vesiculosus ) all showing a high presence of gram negative microbes (and thus LPS). ( Miranda et al, 2022 ; Weigel and Pfister, 2021 ). Other reports show high gram negative presence in the sea kelps ( P. scouleri Laminaria setchellii, N. luetkeana, Chondrus crispus, Laminaria ochroleuca, Palmaria palmate etc.)…”

Section: Discussionmentioning

confidence: 99%

“…Other reports show high gram negative presence in the sea kelps ( P. scouleri Laminaria setchellii, N. luetkeana, Chondrus crispus, Laminaria ochroleuca, Palmaria palmate etc.) Granulosicoccus antarcticus , hellea balneolensis ( Miranda et al, 2022 ; Weigel and Pfister, 2021 ) which reside amongst hundreds of bacterial strains rich in Bacillaceae and Enterobacteriaceae . ( Del Olmo et al, 2018 ; Picon et al, 2021 ) The same is true for coastal ocean sea grasses, with gram-negative biofilms, dominated by Granulosicoccus sp.…”

Section: Discussionmentioning

confidence: 99%

Functional immune boosters; the herb or its dead microbiome? Antigenic TLR4 agonist MAMPs found in 65 medicinal roots and algae’s

Mazzio,

Barnes,

Badisa

et al. 2023

Journal of Functional Foods

View full text Add to dashboard Cite

“…28,109,110 The new kelp holobiont may have been established in China's farming ecosystem. Beneficial microbes can facilitate host growth and, hence, increase yield but at the possible expense of decreased resistance to environmental shifts (e.g., pathogenic infection, 111 ). Therefore, the right kelp-associated microbiome may have the potential to restore the growth-defence trade-off by balancing energy allocation in a farming ecosystem.…”

Section: Priming-based Breedingmentioning

confidence: 99%

Kelp breeding in China: Challenges and opportunities for solutions

Hu,

Shan,

Zhang

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Breeding has played an important role in the mariculture and industrialization of kelp in China. However, the current kelp breeding systems in China have encountered some problems relating to germplasm diversity, management, technological innovations, and regional co‐operation. This review summarizes the main challenges, such as top‐down and fragmented management of germplasm libraries, as well as private industry breeding without government regulations, inter‐cultivar accidental admixing and genetic erosion, loss of heterozygosity due to repeated selection and self‐crossing. We outline multiple potential approaches to breed cultivars with improved qualitative/quantitative traits which can be subjected to changing environments, for example: (i) establishing a national germplasm repository to enhance integrative collection and preservation of kelp resources; (ii) planning and implementing kelp breeding programmes according to strategic priorities and goal‐orientations; (iii) optimizing a hybridization‐based breeding pipeline to produce robust cultivars through the introgression of novel alleles and thus the expression of hybrid vigour; (iv) enriching the high‐quality annotated reference genomes and functional analysis of trait‐associated markers/loci to develop DNA‐based breeding technologies; (v) developing new priming‐based (e.g., thermal and disease resistance) bio‐engineering breeding strategies to meet future unpredictable climate change; and (vi) breeding towards an ecological kelp‐microbiome interaction‐based technique to produce cultivars with enhanced performance and adaptability to environmental scenarios. Collectively, the lessons learned from kelp breeding in China and the solutions proposed here may not only potentially improve or re‐invigorate the Chinese kelp industry, but will also assist other developing countries in taking corrective actions to develop a sustainable future kelp farming industry.

show abstract

The Diversity and Functional Capacity of Microbes Associated with Coastal Macrophytes

Cited by 11 publications

References 99 publications

Successional dynamics of the cultivated kelp microbiome

Successional dynamics of the cultivated kelp microbiome

Functional immune boosters; the herb or its dead microbiome? Antigenic TLR4 agonist MAMPs found in 65 medicinal roots and algae’s

Kelp breeding in China: Challenges and opportunities for solutions

Contact Info

Product

Resources

About