The diversity and distribution of toxigenic Microcystis spp. in present day and archived pelagic and sediment samples from Lake Erie

Rinta-Kanto, Johanna M.; Saxton, Matthew; DeBruyn, Jennifer M.; Smith, Juliette L.; Marvin, Christopher H.; Krieger, Kenneth A.; Sayler, Gary S.; Boyer, Gregory L.; Wilhelm, Steven W.

doi:10.1016/j.hal.2008.08.026

Cited by 72 publications

(41 citation statements)

References 45 publications

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“…Estimates of the MDLs are lower than those reported to date and include microcystins rarely quantified [15,16,26,28], yet significant in lakes and known to cause wildlife mortalities [3,20]. Standard mix injections had RSDs of less than 5%.…”

Section: Rp-hplc-ms/ms Analysis Of Cyanotoxinsmentioning

confidence: 74%

Analysis of intracellular and extracellular microcystin variants in sediments and pore waters by accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry

Zastepa

Pick

Blais

et al. 2015

Analytica Chimica Acta

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Section: Rp-hplc-ms/ms Analysis Of Cyanotoxinsmentioning

confidence: 74%

Analysis of intracellular and extracellular microcystin variants in sediments and pore waters by accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry

Zastepa

Pick

Blais

et al. 2015

Analytica Chimica Acta

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“…In the aftermath of groundbreaking research by Preston et al (1980), who reported that bloom-forming cyanobacterium Microcystis aeruginosa overwintered on sediment surface, numerous studies have indicated that Microcystis can survive in the sediment anywhere from a few days to more than six years (Latour et al, 2007;Misson et al, 2012). In addition to these widely varying time scales, the spatial distribution and preservation of Microcystis has also been determined, with some reports reporting it as a surface sediments and others placing it depths of up to 10-12 cm (Rinta-Kanto et al, 2009). These observations indicate that cyanobacterial organic matter is likely to be a large accumulation in sediments because of the contributions from benthic and pelagic die-off cyanobacteria.…”

Section: Introductionmentioning

confidence: 99%

Predominance of terrestrial organic matter in sediments from a cyanobacteria- blooming hypereutrophic lake

Fujibayashi

et al. 2015

Ecological Indicators

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“…The abundance and diversity of natural populations of cyanobacteria have been investigated in sedimentary archives of perialpine lakes using quantitative PCR and cloning (20)(21)(22). Sedimentary DNA has also been used to investigate the past distribution and diversity of potentially toxic Microcystis in Lake Erie (23) and the saxitoxin-producing Cylindrospermopsis raciborskii in a subtropical lagoon (24).…”

mentioning

confidence: 99%

Sedimentary DNA Reveals Cyanobacterial Community Diversity over 200 Years in Two Perialpine Lakes

Monchamp

Walser

Pomati

et al. 2016

Appl Environ Microbiol

113

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We reconstructed cyanobacterial community structure and phylogeny using DNA that was isolated from layers of stratified sediments spanning 200 years of lake history in the perialpine lakes Greifensee and Lake Zurich (Switzerland). Community analysis based on amplification and sequencing of a 400-nucleotide (nt)-long 16S rRNA fragment specific to Cyanobacteria revealed operational taxonomic units (OTUs) capturing the whole phylum, including representatives of a newly characterized clade termed Melainabacteria, which shares common ancestry with Cyanobacteria and has not been previously described in lakes. The reconstruction of cyanobacterial richness and phylogenetic structure was validated using a data set consisting of 40 years of pelagic microscopic counts from each lake. We identified the OTUs assigned to common taxa known to be present in Greifensee and Lake Zurich and found a strong and significant relationship (adjusted R 2 ‫؍‬ 0.89; P < 0.001) between pelagic species richness in water and OTU richness in the sediments. The water-sediment richness relationship varied between cyanobacterial orders, indicating that the richness of Chroococcales and Synechococcales may be underestimated by microscopy. PCR detection of the microcystin synthetase gene mcyA confirmed the presence of potentially toxic cyanobacterial taxa over recent years in Greifensee and throughout the last century in Lake Zurich. The approach presented in this study demonstrates that it is possible to reconstruct past pelagic cyanobacterial communities in lakes where the integrity of the sedimentary archive is well preserved and to explore changes in phylogenetic and functional diversity over decade-to-century timescales. IMPORTANCECyanobacterial blooms can produce toxins that affect water quality, especially under eutrophic conditions, which are a consequence of human-induced climate warming and increased nutrient availability. Lakes worldwide have suffered from regular cyanobacterial blooms over the last century. The lack of long-term data limits our understanding of how these blooms form. We successfully reconstructed the past diversity of whole cyanobacterial communities over two hundred years by sequencing genes preserved in the sediments of two perialpine lakes in Switzerland. We identified changes in diversity over time and validated our results using existing data collected in the same two lakes over the past 40 years. This work shows the potential of our approach for addressing important ecological questions about the effects of a changing environment on lake ecology. U nderstanding patterns in species diversity across space and time is a fundamental topic in ecological research that has the potential to influence how ecosystems are conserved and managed. In lake ecosystems, eutrophication and warming have favored algal growth and triggered dramatic changes in phytoplankton community composition, such as the dominance of cyanobacteria and the increasing frequency of bloom formation, events that can harm aquatic species and huma...

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The diversity and distribution of toxigenic Microcystis spp. in present day and archived pelagic and sediment samples from Lake Erie

Cited by 72 publications

References 45 publications

Analysis of intracellular and extracellular microcystin variants in sediments and pore waters by accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry

Analysis of intracellular and extracellular microcystin variants in sediments and pore waters by accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry

Predominance of terrestrial organic matter in sediments from a cyanobacteria- blooming hypereutrophic lake

Sedimentary DNA Reveals Cyanobacterial Community Diversity over 200 Years in Two Perialpine Lakes

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