The diverse roles of myeloid derived suppressor cells in mucosal immunity

Ashkenazi‐Preiser, Hadas; Mikula, I.; Baniyash, Michal

doi:10.1016/j.cellimm.2021.104361

Cited by 10 publications

(6 citation statements)

References 214 publications

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“…They are a unique component of the immune system and have a remarkable ability to inhibit the T-cell response. They are immature in the bone marrow and amplified during infection, inflammation, and cancer [ 82 , 83 ]. MDSCs can be classified as granulocyte or polymorphonuclear MDSCs (PMN-MDSCs) and monocyte MDSCs (M-MDSCs).…”

Section: The Role Of Immune Cells In Antibacterial Activity With Biom...mentioning

confidence: 99%

Immunomodulatory biomaterials for implant-associated infections: from conventional to advanced therapeutic strategies

et al. 2022

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Implant-associated infection (IAI) is increasingly emerging as a serious threat with the massive application of biomaterials. Bacteria attached to the surface of implants are often difficult to remove and exhibit high resistance to bactericides. In the quest for novel antimicrobial strategies, conventional antimicrobial materials often fail to exert their function because they tend to focus on direct bactericidal activity while neglecting the modulation of immune systems. The inflammatory response induced by host immune cells was thought to be a detrimental force impeding wound healing. However, the immune system has recently received increasing attention as a vital player in the host’s defense against infection. Anti-infective strategies based on the modulation of host immune defenses are emerging as a field of interest. This review explains the importance of the immune system in combating infections and describes current advanced immune-enhanced anti-infection strategies. First, the characteristics of traditional/conventional implant biomaterials and the reasons for the difficulty of bacterial clearance in IAI were reviewed. Second, the importance of immune cells in the battle against bacteria is elucidated. Then, we discuss how to design biomaterials that activate the defense function of immune cells to enhance the antimicrobial potential. Based on the key premise of restoring proper host-protective immunity, varying advanced immune-enhanced antimicrobial strategies were discussed. Finally, current issues and perspectives in this field were offered. This review will provide scientific guidance to enhance the development of advanced anti-infective biomaterials.

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Section: The Role Of Immune Cells In Antibacterial Activity With Biom...mentioning

confidence: 99%

Immunomodulatory biomaterials for implant-associated infections: from conventional to advanced therapeutic strategies

et al. 2022

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“…(1) the increase in total amount during disease progression; (2) the diversity and plasticity of MDSC subpopulations; (3) their increased suppressive features and functions; and (4) their distinct homing into specific tissue (Ashkenazi-Preiser, Mikula, & Baniyash, 2021;Hou, Hou, Huang, Lei, & Chen, 2020).…”

Section: Commentary Background Informationmentioning

confidence: 99%

“…Thus, identifying novel markers for diagnosis, prognosis, and targeting is imperative for improving anticancer therapies. MDSCs have been reported in various human cancer types and in mouse models depicting a specific pattern regarding: (1) the increase in total amount during disease progression; (2) the diversity and plasticity of MDSC subpopulations; (3) their increased suppressive features and functions; and (4) their distinct homing into specific tissue (Ashkenazi‐Preiser, Mikula, & Baniyash, 2021; Hou, Hou, Huang, Lei, & Chen, 2020).…”

Section: Commentarymentioning

confidence: 99%

Phenotypic Characterization and Isolation of Myeloid‐Derived Suppressor Cells

Reuven¹,

Mikula²,

Ashkenazi‐Preiser³

et al. 2022

Current Protocols

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Myeloid‐derived suppressor cells (MDSCs) are heterogenous populations of immature myeloid cells that can be divided into two main subpopulations, polymorphonuclear (PMN) MDSCs and monocytic (M) MDSCs. These cells accumulate during chronic inflammation and induce immunosuppression evident in an array of pathologies such as cancer, inflammatory bowel disease, and infectious and autoimmune diseases. Herein, we describe methods to isolate and characterize MDSCs from various murine tissue, as well as to phenotype blood‐derived MDSCs from patients. The protocols describe methods for isolation of total MDSCs and their subpopulations, for characterization, and for evaluation of their distribution within tissue, as well as for assessing their maturation stage by flow cytometry, immunofluorescence analyses, and Giemsa staining. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Single‐cell suspension generation from different tissue Alternate Protocol 1: Single‐cell suspension generation from subcutaneous melanoma tumors Basic Protocol 2: Characterization of MDSC phenotype Basic Protocol 3: Cell separation using magnetic beads: Separating pan‐MDSCs or PMN‐MDSC and M‐MDSC subpopulations Alternate Protocol 2: Staining and preparing MDSCs for sorting Support Protocol: PMN‐MDSC and M‐MDSC gating strategy in mouse Basic Protocol 4: Immunofluorescence analysis of MDSCs Basic Protocol 5: Handling human blood samples and characterizing human MDSCs Alternate Protocol 3: Flow cytometry staining of thawed human whole blood samples

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“…MDSCs have been reported in various human cancer types and mouse models and display unique properties. These properties include increased total number during disease progression, enhanced suppressive features and functions, distinct homing to specific tissue, and diversity and plasticity (Ashkenazi‐Preiser, Mikula, & Baniyash, 2021; Hou, Hou, Huang, Lei, & Chen, 2020). MDSC immunosuppressive features are characterized by elevated levels of secreted ROS and NO, production of proinflammatory cytokines, and enhanced activity of arginase‐1, which results in the deprivation of arginine and cysteine from the environment.…”

Section: Commentarymentioning

confidence: 99%

Functional Assays Evaluating Immunosuppression Mediated by Myeloid‐Derived Suppressor Cells

Reuven¹,

Mikula²,

Ashkenazi‐Preiser³

et al. 2022

Current Protocols

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Myeloid-derived suppressor cells (MDSCs) are heterogenous populations of immature myeloid cells that can be divided into two main subpopulations, polymorphonuclear (PMN) MDSCs and monocytic (M) MDSCs. These cells accumulate during chronic inflammation, characterizing an array of pathologies such as cancer, inflammatory bowel disease, and infectious and autoimmune diseases, and induce immunosuppression. The suppressive effects of MDSCs on the immune system are studied mainly when focusing on their features, functions, and impact on target cells such as T cells, natural killer cells, and B cells, among others. Herein, we describe methods for the analysis of MDSC immunosuppressive features and functions, measuring different mediators that contribute to their activities and how they impact on T cell function. The protocols described are a continuation to those in a companion Current Protocols article by Reuven et al. (2022), which uses a generated single-cell suspension and isolated cells to test their activity.

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The diverse roles of myeloid derived suppressor cells in mucosal immunity

Cited by 10 publications

References 214 publications

Immunomodulatory biomaterials for implant-associated infections: from conventional to advanced therapeutic strategies

Immunomodulatory biomaterials for implant-associated infections: from conventional to advanced therapeutic strategies

Phenotypic Characterization and Isolation of Myeloid‐Derived Suppressor Cells

Functional Assays Evaluating Immunosuppression Mediated by Myeloid‐Derived Suppressor Cells

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