The diverse community of leaf-inhabiting fungal endophytes from Philippine natural forests reflects phylogenetic patterns of their host plant species Ficus benjamina, F. elastica and F. religiosa

Solis, Michael Jay L.; Cruz, Thomas Edison E. dela; Schnittler, Martin; Unterseher, Martin

doi:10.1016/j.myc.2015.10.002

Cited by 36 publications

(30 citation statements)

References 59 publications

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“…Schulz, Boyle, Draeger, Rommert, and Krohn () found that approximately 80% of fungal endophyte species produce secondary compounds with antifungal activity, which mediated the competition among fungal species (Saunders et al, ). Previous studies have also indicated that different host species show distinct FEF assemblages (Higgins et al, ; Solis, Dela Cruz, Schnittler, & Unterseher, ; Vincent, Weiblen, & May, ). An early study of FEF assemblages on coniferous trees found that many endophytes were restricted to a single or a restricted number of host species (Carroll & Carroll, ).…”

Section: Introductionmentioning

confidence: 91%

“…Ficus have been recognized as a keystone species in tropical rainforests because they provide a year‐round food source for many frugivorous animals (Sanitjan & Chen, ; Shanahan, So, Compton, & Corlett, ). Two studies on the FEF of Ficus (Solis et al, ; Vincent et al, ) have suggested that host identity affects FEF assemblages. In this study, we used the fig collection in the Xishuangbanna Tropical Botanical Garden (XTBG), in which all fig trees grow in a small area (1.3 ha), in the same seminatural environment, in a humid tropical climate, and are exposed to the same community of herbivores and pathogens, to address the following questions: (a) does host identity influence FEF assemblages; (b) does phylogeny of host plants significantly affect FEF assemblages; and (c) do leaf traits filter FEF and contribute to explain the variation in FEF assemblages.…”

Section: Introductionmentioning

confidence: 99%

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Host identity and phylogeny shape the foliar endophytic fungal assemblages of Ficus

Liu

Zhao

Wang

et al. 2019

Ecology and Evolution

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Foliar endophytic fungi (FEF) are diverse and ubiquitously associated with photosynthetic land plants. However, processes shaping FEF assemblages remain poorly understood. Previous studies have indicated that host identity and host habitat are contributing factors, but these factors are often difficult to disentangle. In this study, we studied FEF assemblages from plants grown in a botanical garden, enabling us to minimize the variation in abiotic environmental conditions and fungal dispersal capacity. FEF assemblages from 46 Ficus species were sequenced using next‐generation methods, and the results indicated that closely related host species had clearly differentiated FEF assemblages. Furthermore, host phylogenetic proximity was significantly correlated with the similarity of their FEF assemblages. In the canonical correspondence analysis, eleven leaf traits explained 32.9% of the total variation in FEF assemblages, whereas six traits (specific leaf area, leaf N content, leaf pH, toughness, latex alkaloid content, and latex volume per leaf area) were significant in the first two dimensions of ordination space. In the multiple regression on distance matrix analysis, 21.0% of the total variance in FEF assemblage was explained by both host phylogeny and leaf traits while phylogeny alone explained 7.9% of the variance. Thus, our findings suggest that both evolutionary and ecological processes are involved in shaping FEF assemblages.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Host identity and phylogeny shape the foliar endophytic fungal assemblages of Ficus

Liu

Zhao

Wang

et al. 2019

Ecology and Evolution

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“…Cultivation of endophytes using the dilution-to-extinction method Isolation of endophytes followed the dilution-to-extinction cultivation (Solis et al 2016). In brief, samples were blended into tiny particles and filtered.…”

Section: Study Sites and Samplingmentioning

confidence: 99%

“…Solis et al 2016). The ITS region was amplified with the primer pair V9G -ITS4 (de Hoog and Gerrits van den Ende 1998) using approved amplification kits (MangoTaq; Bioline, Germany) and cycling conditions (Solis et al 2016). Unpurified PCR products were shipped to Beckman Coulter Genomics (Takeley, UK) for sequencing.…”

Section: Dna Extraction and Its Sequencing From Axenic Culturesmentioning

confidence: 99%

What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions

Siddique¹,

Khokon²,

Unterseher³

2017

Self Cite

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Comparative simultaneous studies of environmental high-throughput sequencing (HTS) and cultivation of plant-associated fungi have rarely been conducted in the past years. For the present contribution, HTS and extinction culturing were applied for the same leaf samples of European beech (Fagus sylvatica) in order to trace both "real" environmental drivers as well as method-dependent signals of the observed mycobiomes. Both approaches resulted in non-overlapping community composition and pronounced differences in taxonomic classification and trophic stages. However, both methods revealed similar correlations of the fungal communities with local environmental conditions. Our results indicate undeniable advantages of HTS over cultivation in terms of revealing a good representation of the major functional guilds, rare taxa and biodiversity signals of leaf-inhabiting fungi. On the other hand our results demonstrate that the immense body of literature about cultivable endophytic fungi can and should be used for the interpretation of community signals and environmental correlations obtained from HTS studies and that cultivation studies should be continued at the highest standards, e.g. when sequencing facilities are not available or if such surveys are expanded into functional aspects with experiments on living isolates.

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“…The ecology of this recently described species (Crous et al., ) is poorly known, because it has been described based on a single strain isolated from the leaves of a fern in Thailand. It is evident from the ITS rDNA sequences with 99–100% similarity deposited in the GenBank database that P. hymenocallidicola is predominantly an endophytic fungus with a broad spectrum of host plants, including Ardisia (Higginbotham et al., ), Eucalyptus (Crous et al., ; Marsberg, Slippers, Wingfield, & Gryzenhout, ), Ficus (Solis, Cruz, Schnittler, & Unterseher, ), Heterosmilax (Gao et al., ), Livistona (Guo, Hyde, & Liew, ), Minosops (Sopalun, ), Phryganocydia (Higginbotham et al., ) and Psychotria (Higginbotham et al., ). Phaeophleospora hymenocallidicola has also been isolated from mangrove soil (Xu, Yu, Liu, Li, & Wang, ), coral mucus (Barathikannan, Ramasamy, Manohar, & Meena, ), deep‐sea sediment (Zhang et al., ) and hypersaline microbial mats (Cantrell et al., ).…”

Section: Discussionmentioning

confidence: 99%

Swim bladder mycosis in pretty tetra (Hemigrammus pulcher) caused by Exophiala pisciphila and Phaeophleospora hymenocallidicola, and experimental verification of pathogenicity

Řehulka

Kubátová

Hubka

2017

Journal of Fish Diseases

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Spontaneous invasive and chronic disseminated mycosis affected Hemigrammus pulcher kept in a public aquarium, and infection was manifested by inappetence, exophthalmia, erratic swimming, eroded scales, anaemia of the gills and abdominal distension. Internally, there was a grossly swollen swim bladder with a thickened wall filled with a dark mass. The body cavities contained a clear, light amber fluid and a swollen intestine which was full of a watery fluid containing small gas bubbles. Histopathology revealed a granulomatous inflammatory response with fungal hyphae in the lumen and wall of the swim bladder, hepatopancreas, spleen and kidneys with signs of nephrohydrosis. Exophiala pisciphila and Phaeophleospora hymenocallidicola were isolated from the swim bladder, abdominal cavity and gastrointestinal tract. The exogenous source of infection was probably the ample wooden decoration and plants inside the aquarium. Koch's postulates were fulfilled by re-isolation of both fungal species from fish artificially infected under laboratory conditions. As P. hymenocallidicola is less capable of defence against phagocytosis, E. pisciphila probably played a major role. Severe clinical manifestations with 100% mortality developed in two fish species infected by E. pisciphila. A significant increase in the plasma levels of amino acids was observed as a result of the activation of proteolysis.

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The diverse community of leaf-inhabiting fungal endophytes from Philippine natural forests reflects phylogenetic patterns of their host plant species Ficus benjamina, F. elastica and F. religiosa

Cited by 36 publications

References 59 publications

Host identity and phylogeny shape the foliar endophytic fungal assemblages of Ficus

Host identity and phylogeny shape the foliar endophytic fungal assemblages of Ficus

What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions

Swim bladder mycosis in pretty tetra (Hemigrammus pulcher) caused by Exophiala pisciphila and Phaeophleospora hymenocallidicola, and experimental verification of pathogenicity

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