The diverse and extensive plant polysaccharide degradative apparatuses of the rumen and hindgut Prevotella species: A factor in their ubiquity?

Accetto, Tomaž; Avguštin, Gorazd

doi:10.1016/j.syapm.2018.10.001

Cited by 51 publications

(48 citation statements)

References 46 publications

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“…Utilization of xylan, which is found in cereal grains, has been repeatedly established in Prevotella species and in P. copri specifically (Accetto and Avgustin, 2019;da Silva et al, 2012;Dodd et al, 2011;Tan et al, 2018). Two distinct PULs encoding enzymes not previously associated with xylan catabolism were identified for corn xylan, birchwood xylan, and arabinoxylan in multiple B. ovatus and B. xylanisolvens strains (Rogowski et al, 2015).…”

Section: Prevotella Polysaccharide Utilizationmentioning

confidence: 94%

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Distinct polysaccharide growth profiles of human intestinalPrevotella copriisolates

Fehlner-Peach

Magnabosco

Raghavan

et al. 2019

Preprint

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The gut-dwelling Prevotella copri, the most prevalent Prevotella species in human gut, have been associated with diet as well as disease, but our understanding of their diversity and functional roles remains rudimentary, as studies have been limited to 16S and metagenomic surveys and experiments using a single type strain. Here, we describe the genomic diversity of 83 P. copri isolates from 11 donors. We demonstrate that genomically distinct isolates, which can be categorized into different P. copri complex clades, utilize defined sets of plant polysaccharides that can be predicted in part from their genomes and from metagenomic data.This study thus reveals both genomic and functional differences in polysaccharide utilization between several novel human intestinal P. copri strains.

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Section: Prevotella Polysaccharide Utilizationmentioning

confidence: 94%

“…Prevotella isolates from livestock grow in the presence of plant polysaccharides and encode gene products capable of breaking down these polysaccharides (Accetto and Avgustin, 2019). Thus, diet may be an important factor promoting growth of intestinal Prevotella.…”

mentioning

confidence: 99%

Distinct polysaccharide growth profiles of human intestinalPrevotella copriisolates

Fehlner-Peach

Magnabosco

Raghavan

et al. 2019

Preprint

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“…Rumen microbial community was usually dominated by Prevotella at genus level when fed high grain diets [43]. Members of Prevotella are able to utilise various nutrients such as starch, proteins and noncellulosic polysaccharides [44,45], and to convert lactate into propionate to prevent accumulation of lactate [40]. Moreover, as Prevotella are also H 2 -consuming bacteria in addition to Selenomonas [46], the reduced H 2 with FlaH and AlcH was likely due to the increased relative abundance of genus Prevotella.…”

Section: Effects Of Alch and Flah On Microbial Protein Synthesis And mentioning

confidence: 99%

Effects of Brewers’ Spent Grain Protein Hydrolysates on Gas Production, Ruminal Fermentation Characteristics, Microbial Protein Synthesis and Microbial Community in an Artificial Rumen Fed a High Grain Diet

Ran

Jin

Abeynayake

et al. 2020

Preprint

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Background: Brewers’ spent grain (BSG) contains 20 ~ 29% protein with high amount of glutamine, proline and hydrophobic and non-polar amino acid residues, making it an ideal material for producing value-added products like bioactive peptides. Protein were extracted from BSG, hydrolyzed with 1% alcalase and flavourzyme, and the generated protein hydrolysates (AlcH and FlaH) showed antioxidant activities. This study was conducted to evaluate effects of AlcH and FlaH on gas production, fermentation characteristics, nutrient disappearance, microbial protein synthesis and microbial community using an artificial rumen system (RUSITEC) fed a high-grain diet.Results: Supplementation of FlaH decreased (P < 0.01) disappearances of dry matter (DM), organic matter (OM), crude protein (CP) and starch, without affecting fibre disappearances; while AlcH had no effect on nutrient disappearance. Neither AlcH nor FlaH affected gas production and VFA profiles, except they enhanced (P < 0.01) NH3-N but decreased (P < 0.01) H2 production. Supplementation of FlaH decreased (P < 0.01) percentage of CH4 in total gas and dCH4 in dissolved gas. Addition of monensin reduced (P < 0.01) nutrient disappearances, improved fermentation efficiency and reduced CH4 and H2 emission. Total microbial nitrogen production decreased (P < 0.05) but proportion of feed particle associated (FPA) bacteria increased with FlaH and monensin. Numbers of OTUs or Shannon diversity indices of FPA microbial community were unaffected by AlcH and FlaH; whereas both indices were reduced (P < 0.05) by monensin. Taxonomic analysis revealed no effect of AlcH and FlaH on the relative abundance (RA) of bacteria at phylum level; monensin reduced (P < 0.05) the RA of Firmicutes and Bacteroidetes and enhanced Proteobacteria. The FlaH enhanced (P < 0.05) the RA of genus Prevotella, reduced Selenomonas, Shuttleworthia, Bifidobacterium and Dialister as compared to control; monensin reduced (P < 0.05) RA of genus Prevotella but enhaced Succinivibrio.Conclusions: Inclusion of FlaH in high-grain diet may potentially protect CP and starch from ruminally degradation, without adversely affecting fibre degradation and VFA profiles. The FlaH also showed promising effects on reducing CH4 production by suppressing H2 generation. Protein enzymatic hydrolysates from BSG using flavourzyme showed potential application to high value-added bio-products.

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“…Many of the CAZy were unanimously identified only on GH family level, but a number of CAZy in Bacteroides and Prevotella CAZy profiles were identified even by their actual enzyme names. According to these annotations, enzymes in both the Bacteroides and Prevotella CAZy profiles were predicted to have activities against complex polysaccharides of either plant cell wall or animal glycan origin 6,35 , and to some extent, against simpler carbohydrates. Pathway analysis using KEGG orthologs indicated that enzymes in the Prevotella CAZy cluster participated in various carbohydrate degradation pathways, many of them in degradation of pectin.…”

Section: Discussionmentioning

confidence: 99%

A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota

Aakko

Pietilä

Toivonen

et al. 2020

Sci Rep

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Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being. Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment. We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging. We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects. In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-larabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides. This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy. Identification of Bacteroides-and Prevotella-derived cAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.

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The diverse and extensive plant polysaccharide degradative apparatuses of the rumen and hindgut Prevotella species: A factor in their ubiquity?

Cited by 51 publications

References 46 publications

Distinct polysaccharide growth profiles of human intestinalPrevotella copriisolates

Distinct polysaccharide growth profiles of human intestinalPrevotella copriisolates

Effects of Brewers’ Spent Grain Protein Hydrolysates on Gas Production, Ruminal Fermentation Characteristics, Microbial Protein Synthesis and Microbial Community in an Artificial Rumen Fed a High Grain Diet

A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota

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