The diuretic activity of a series of cephalomyotropic neuropeptides, the achetakinins, on isolated Malpighian tubules of the house cricket, Acheta domesticus

An antidiuretic peptide (Tenmo-ADFb) with kinin-like diuretic activity on Malpighian tubules of the house cricket, Acheta domesticus (L.)

“…The apparent EC 50 is 10.2·pmol·l . This compares with an EC 50 of 22·pmol·l -1 for Achdo-KII when assayed in cricket saline containing 9.6·mmol·l -1 K + (Coast et al, 1990). of six residues from the N-terminus had no effect on activity.…”

“…AMP Synergism between kinins and cyclic AMP has been described in several insects, notably A. domesticus (Coast et al, 1990), Locusta migratoria (Coast, 1995) and M. domestica (Holman et al, 1999), and can be attributed to the separate activation of anion and cation transport processes, respectively. To investigate possible synergism between TenmoADFb and 8-bromo-cyclic AMP, tubules were challenged with each of the secretagogues separately and together.…”

Effects of leucokinin-VIII onAedesMalpighian tubule segments lacking stellate cells

“…In Drosophila Malpighian tubules, stellate cells mediate the effects of drosokinin, the Drosophila leucokinin (Radford et al, 2002;Terhzaz et al, 1999). In the house cricket Acheta domesticus, principal cells must by default mediate the effects of achetakinins (the Acheta leucokinin) because Acheta Malpighian tubules do not possess stellate cells (Coast et al, 1990;Hazelton et al, 1988). However, Malpighian tubules of the blood-sucking bug Rhodnius prolixus, which also lack stellate cells, do not respond to leucokinin (Bradley, 1983;Te Brugge et al, 2002).…”

“…Curious that agents which stimulate hindgut contractions might also enhance excretory activity in Malpighian tubules upstream, we discovered that leucokinin has diuretic effects in Malpighian tubules of the yellow fever mosquito Aedes aegypti (Hayes et al, 1989). Since then, the stimulation of fluid secretion by leucokinin and other kinins has been found in Malpighian tubules of the house cricket (Coast et al, 1990), locust (Thompson et al, 1995), tobacco hornworm (Blackburn et al, 1995), fruit fly (O'Donnell et al, 1996) and housefly (Iaboni et al, 1998). In Malpighian tubules of the yellow fever mosquito, locust and cricket, leucokinin is thought to increase transepithelial secretion of Cl -, thereby increasing the transepithelial secretion of Na + , K + and water (Coast, 2001;Pannabecker et al, 1993).…”

Intracellular Na+, K+ and Cl− activities in Acheta domesticus Malpighian tubules and the response to a diuretic kinin neuropeptide

“…Malpighian tubules of the house cricket, Acheta domesticus Linnaeus 1758, lack stellate cells (Hazelton et al, 1988), and kinins must therefore bind receptors on principal cells to stimulate fluid secretion and depolarize V t (Coast et al, 1990;Coast et al, 2007). As in D. melanogaster and A. aegypti tubules, Ca 2+ is used as second messenger in opening a transepithelial Cl -conductance pathway, leading to the non-selective stimulation of NaCl and KCl transport (Coast et al, 2007;Coast, 2011).…”