The Distribution of Transplanted Umbilical Cord Mesenchymal Stem Cells in Large Blood Vessel of Experimental Design With Traumatic Brain Injury

Abstract

The authors aim to track the distribution of human umbilical cord mesenchymal stem cells (MSCs) in large blood vessel of traumatic brain injury -rats through immunohistochemical method and small animal imaging system. After green fluorescent protein (GFP) gene was transfected into 293T cell, virus was packaged and MSCs were transfected. Mesenchymal stem cells containing GFP were transplanted into brain ventricle of rats when the infection rate reaches 95%. The immunohistochemical and small animal imaging system was used to detect the distribution of MSCs in large blood vessels of rats. Mesenchymal stem cells could be observed in large vessels with positive GFP expression 10 days after transplantation, while control groups (normal group and traumatic brain injury group) have negative GFP expression. The vascular endothelial growth factor in transplantation group was higher than that in control groups. The in vivo imaging showed obvious distribution of MSCs in the blood vessels of rats, while no MSCs could be seen in control groups. The intravascular migration and homing of MSCs could be seen in rats received MSCs transplantation, and new angiogenesis could be seen in MSCs-transplanted blood vessels.

Resveratrol pretreatment attenuates traumatic brain injury in rats by suppressing NLRP3 inflammasome activation via SIRT1

“…TBI model. The detailed methods to produce TBI model had been described previously (1). Briefly, we used a free fall device in the experiment.…”

“…Traumatic brain injury (TBI) is a common severe injury of neurosurgery, which is mainly caused by mechanical forces of tearing, shearing or stretching. TBI has been a heavy economic and social burden and has high morbidity and mortality (1). So far plenty of researches have been conducted to illuminate the mechanisms underlying TBI and explored the effective neuroprotective drugs aiming to alleviate the grave consequences of TBI.…”

Transplantation of Bone Marrow Mesenchymal Stem Cells Prevents Radiation‐Induced Artery Injury by Suppressing Oxidative Stress and Inflammation

“…MSCs orchestrate the repair process of injured vessels by various mechanisms such as transdifferentiation, microvesicles or exosomes, and secreting cytokines [ 13 , 14 ]. MSCs can directly differentiate into endothelial cells to participate in angiogenesis [ 15 ] or migrate and home to the injured large blood vessel for vascular repair by regulating various cell cytokines, such as transforming growth factor beta (TGF- β ), vascular endothelial growth factor (VEGF), and intercellular cell adhesion molecules (ICAM) [ 16 , 17 ].…”

A New Approach to Cerebral Palsy Treatment: Discussion of the Effective Components of Umbilical Cord Blood and its Mechanisms of Action

“…The distribution of MSCs can be detected after transplantation of green fluorescent protein (GFP)-containing cells into the rat ventricles. Ten days after transplantation, MSCs with positive GFP expression could be seen in large vessels, and vascular endothelial growth factors were also higher in the transplanted group than in the control group 54 . In addition, Rahimzadeh et al reported that transplantation of autologous MSCs in arrangement by HSCs improved HSC engraftment 49 and resulted in the production of anti-inflammatory macrophages for increasing tissue repair 55 .…”