The Distribution of ¹²⁵I‐Marked Bovine Prolactin and Human Chorionic Gonadotrophin in Rats with Experimental Ovarian Tumours

Abstract

The distribution and fate of 1251-labelled bovine prolactin, human chorionic gonadotrophin and bovine serum albumin was studied and followed by serial gamma camera photographs, scintillation counting of blood and tissues, and autoradiography in normal pro-oestrus rats, in oophorectomized rats and in rats with experimental ovarian tumours developed in intrasplenic ovarian grafts after bilateral oophorectomy. A high affinity for T -H C G was found both in ovarian tumours and in normal ovaries. An increased active tumour uptake of Iz6I-HCG was observed for 3 hours, which permitted tumour depicting with gamma-camera technique. The 1251-prolactin uptake was more moderate both in ovaries and ovarian tumours. 1251-albumin showed no specific affinity. The possible application of these findings in diagnosis and therapy of human ovarian tumours is discussed.10. -

Uptake of 125i-Labelled Human Placental Lactogen and Human Placental Lactogen by the Tissues of Normal and Lactating Rats

“…injection of 125I-labelled protein hormones into animals or by the incubation of the target organ with the labelled hormone. Human chorionic gonadotrophin (HCG) (Eshkol & Lunenfeld, 1968;Carlsson, Kullander & Müller, 1972;Catt, Tsuruhara & Dufau, 1972;Danzo, Midgley & Kleinsmith, 1972;Leidenberger & Reichert, 1972;Yaginuma, 1972), prolactin (Birkinshaw & Falconer, 1972;Shani (Mishkinsky), Givant, Sulman, Eshkol & Lunenfeld, 1972) and luteinizing hormone (LH) (Barofsky, Lipner & Armstrong, 1971) iodinated by the standard chloramine-T method, all retained sufficient biological activity to be taken up and bound to target receptors. Recently, Moodbidri, Sheth & Rao (1973) have shown that HPL radioiodinated by the modified method of Greenwood et al (1963) was bound to membranes of the buffalo corpus luteum.…”

Antitumor Effects of Analogs of Hypothalamic Hormones in Endocrine-Dependent Cancers

“…The majority of ovarian cancers are of epithelial origin. A similar dependency on gonadotropins may occur in certain human ovarian tumors (125)(126)(127). Most common functioning ovarian neoplasms are referred to as granulosa-theca cell tumors (121,122).…”

“…Ovarian cancer is not a single entity, but encompasses numerous cell types ( 12 1 ). Animal models of granulosa cell tumors are dependent on hypophyseal gonadotropins for stimulation of hormone secretion, and concentrate '251-labeled HCG (125)(126)(127). The primary treatment of ovarian cancer is surgical removal ( 12 l), but chemotherapy is more commonly used today in the management of disseminated ovarian carcinoma because of unsatisfactory results following operation alone ( 12 1).…”

Distribution of ¹²⁵I-Prolactin in Mice and Rats. Studies with Whole-Body and Microautoradiography

“…One possibility to explore the possible target organs as well as the sites of metabolic inactivation and/or excretion is to use auto radiographic techniques with labeled hormone. In a recent study, gamma camera and autoradiography have indicated that 125I-prolactin shows only a slight accumulation in the ovary or splenic ovarian tumors [Carlsson et al, 1972], We have chosen a similar approach using whole-body and microautoradiographic techniques to evaluate the distribution and time-course of disappearance of labeled prolactin in normal cyclic, pregnant and lactating female mice as well as in organs of normal male mice and rats.…”