THE DISTRIBUTION OF RADIOACTIVE myo-INOSITOL IN THE REPRODUCTIVE TRACT OF THE MALE RAT. THE BLOOD-TESTIS BARRIER TO myo-INOSITOL

Lewin, L.; Yannai, Y.; Kraicer, P. F.

doi:10.1016/b978-0-12-741750-9.50028-8

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…which contains high levels of inositol. W e have previously reported that the secretory organs of the male reproductive tract of the rat, with the exception of the testis, were sites of active uptake of injected tritiated inositol, and we provided evidence that the coagulating gland, seminal vesicle and prostate glands secreted the radioactive inositol into their respective glandular fluids (Lewin & Sulimovici 1975;Lewin ei al. 1976).…”

supporting

confidence: 56%

The Effect of Cyproterone Acetate on Myo‐Inositol Uptake and Secretion in the Reproductive Tract of the Male Rat

1979

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A procedure is described for evaluating the uptake of myo‐inositol by measuring the radioactivity (dpm/mg wet weight of tissue) in the male reproductive accessory glands after injection of (2‐3H)myo‐inositol into bilaterally nephrectomized rats. The rate of secretion of the labelled inositol was assessed by measuring the total dpm in fluids expressed from the seminal vesicles and coagulating glands after injection of the tritiated inositol. Cyproterone acetate treatment (15 mg/day), for 2 to 7 days, inhibited secretion of radioactive inositol by the seminal vesicles and decreased the uptake capacity to a greater extent than it inhibited the rate of inositol accumulation into these glands. It had a similar effect on the coagulating glands after 7 days of treatment. The prostate was the least affected by the cyproterone acetate treatment.

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confidence: 56%

The Effect of Cyproterone Acetate on Myo‐Inositol Uptake and Secretion in the Reproductive Tract of the Male Rat

1979

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“…In the trichloroacetic acid extracts of all organs studied, the amount of radioactivity found in free inositol greatly predominated over that associated with its metabolites. Radioactive inositol was mainly in the high-speed supernatant fraction isolated from testis, prostate, and seminal vesicles and particulate bound in the microsomes and mitochondria from liver (Lewin and Sulimovici, 1975).…”

Section: Digestion and Absorptionmentioning

confidence: 99%

The Nutritional Significance, Metabolism, and Function of myo-Inositol and Phosphatidylinositol in Health and Disease

Holub

1982

Advances in Nutritional Research

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“…The great capacity of the testis to produce inositol may be a consequence of the blood/testis barrier, which prevents the entry of certain blood substances including inositol (20). To maintain the high concentration of inositol found in the rete testis (100 times the plasma concentration), the organism requires a nearby synthetic system (21).…”

Section: Figurementioning

confidence: 99%

Selective hormonal control of myo-inositol biosynthesis in reproductive organs and liver of the male rat.

Hasegawa¹,

Eisenberg²

1981

Proc. Natl. Acad. Sci. U.S.A.

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myo-Inositol biosynthesis has been examined in hypophysectomized and thyroidectomized male rats. After hypophysectomy, inositol-l-phosphate synthase [IL-myo-inositol-lphosphate lyase (isomerizing), EC 5.5. 1.4] Among the many tissues capable of synthesizing myo-inositol, the testis ranks as the richest mammalian source of inositol-1-phosphate synthase [lL-myo-inositol-l-phosphate synthase, EC 5.5.1.4], the NAD+-dependent enzyme that isomerizes glucose 6-phosphate to myo-inositol 1-phosphate (1) referred to here as simply inositol 1-phosphate. Because the testis is the target organ for various hormones and is itself the site of androgen biosynthesis, we were led to inquire into possible hormonal control of inositol biosynthesis. Although there have been sporadic reports on the effects of various physiological and pathological states on the levels of free inositol and inositol-l-phosphate synthase in certain tissues (2-7), there has been no systematic study of hormonal control of the enzyme. Here we report the effect in male rats of hypophysectomy, adrenalectomy, and thyroidectomy on tissue levels of inositol-l-phosphate synthase and preliminary experiments in the reversal of these effects by gonadotropic and thyroid hormones. MATERIALS AND METHODSMale Sprague-Dawley rats weighing approximately 200 g were obtained from Taconic Farms (Germantown, NY) and maintained as follows. Normal animals received regular tap water; hypophysectomized, 5% dextrose; thyroidectomized, 1% calcium lactate; and adrenalectomized, isotonic saline. Sham-operated rats were given the same fluids; all animals received regular laboratory chow ad lib. Tissues for enzyme assay were removed and weighed immediately after killing by decapitation. Seminal vesicles were washed with cold isotonic saline after removal of seminal fluid through an incision. Brain, liver, spleen, thymus, pancreas, kidney, testis, and ventral prostate were homogenized in a Potter-Elvehjem homogenizer with 2-3 vol of 0.154 M KCI/0.2 mM dithiothreitol. Epididymis and seminal vesicles were minced and then homogenized in a Polytron homogenizer with 3-5 vol of the same solution. The homogenates were centrifuged 20 min at 27,000 X g, and the supernatants were heated at 60'C for 2 min exactly, chilled in ice, and centrifuged at 105,000 x g for 1 hr. Solid ammonium sulfate was added to 40% of saturation (0.226 g/ml). The precipitate was collected by centrifugation, dissolved in 0.6-0.7 ml of 50 mM Tris acetate, pH 7.4/0.2 mM dithiothreitol and dialyzed against the same buffer overnight. The suspension was freed of insoluble material by centrifugation and assayed for inositol-1-phosphate synthase by incubation with glucose 6-phosphate (5 mM) and NAD' (1 mM); the resulting inositol 1-phosphate was determined as inorganic phosphate by the periodate method of Barnett et al. (8). Protein was determined by the method of Lowry et al. (9), with correction for the effect ofTris.

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THE DISTRIBUTION OF RADIOACTIVE myo-INOSITOL IN THE REPRODUCTIVE TRACT OF THE MALE RAT. THE BLOOD-TESTIS BARRIER TO myo-INOSITOL

Cited by 5 publications

References 11 publications

The Effect of Cyproterone Acetate on Myo‐Inositol Uptake and Secretion in the Reproductive Tract of the Male Rat

The Effect of Cyproterone Acetate on Myo‐Inositol Uptake and Secretion in the Reproductive Tract of the Male Rat

The Nutritional Significance, Metabolism, and Function of myo-Inositol and Phosphatidylinositol in Health and Disease

Selective hormonal control of myo-inositol biosynthesis in reproductive organs and liver of the male rat.

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