The distribution of radioactive carbon from (U-14C)glucosamine in mice.

Goto, Hideyuki; Watanabe, Masaru; Shimono, Ryuichi; Kihara, Takashi

doi:10.1267/ahc.17.213

Cited by 11 publications

(14 citation statements)

References 23 publications

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“…The general pattern of the autoradiographs obtained, especially at the late intervals, after injection of radioactive tyrosine had a resemblance to those reported about tryptophan (9) and other amino acids (1,3,12). This `amino acid pattern' was relatively similar to that about dopa (6,22) and different from those about hexoses (13,21) and hexosamines (7,8).…”

Section: Discussionmentioning

confidence: 67%

“…The mice were injected intravenously with 10 iCi of radioactive tyrosine and sacrificed in groups of three at 5 , 30, 60 and 180 min after injection. Whole-body sagittal sections (25 (gym thick) of the mice were prepared according to the methods in a previous report (7) . Autoradiography of sections was performed before and after incubation with 6% perchloric acid in the manner previously reported (24).…”

Section: Methodsmentioning

confidence: 99%

“…The blood, brain, Harderian gland, salivary gland, thymus, lung, cardiac muscle, skeletal muscle, stomach, small intestine, large intestine, liver, pancreas, spleen, kidney, seminal vesicle and testis were immediately removed. The acid-soluble, acidinsoluble and lipid fractions of each organ were obtained by the methods described in a previous report (7) except that homogenization was carried out with 6% perchloric acid instead of with 10% trichloroacetic acid. The radioactivity of each fraction was determined by a Packard liquid scintillation counter.…”

Section: Methodsmentioning

confidence: 99%

“…The distribution and metabolic fates of tyrosine have been extensively studied, but not satisfactorily in the whole-body. In the meantime, whole-body autoradiography has been used for investigation of the distributions of hexoses (13,21) and hexosamines (7,8) in mice in our laboratory. In the recent report (9), the distribution of radioactive tryptophan and/or its metabolites in mice was examined by this method.…”

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confidence: 99%

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The distribution of radioactive carbon from L-(U-14C)tyrosine in mice.

Goto

Matsushima

Kanayama

et al. 1987

Acta Histochem. Cytochem

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Tissue and organ distribution of radioactive carbon from 14C-tyrosine in the mouse was studied by whole-body autoradiography and biochemical analysis. The mice injected intravenously with L-[U-14C]tyrosine were sacrificed at various intervals. Examination of autoradiographs disclosed that the injected 14C-tyrosine was rapidly taken up from the blood by the tissues. The radioactivity in the pancreas was the highest and predominant throughout the intervals after injection in this investigation.The comparative values among radioactivity in the organs estimated by a liquid scintillation counter were approximately consistent with those obtained from the whole-body autoradiographs. Radioactivity in the acid-insoluble fractions was increased with time in all organs examined. High-performance liquid chromatography of the acid-soluble fractions disclosed that the amounts of the radioactive tyrosine and its metabolites and/or their molar ratios were different among the organs.Tyrosine is distributed in tissues and body fluids, in a free form and incorporated into protein molecules. It is generally accepted that tyrosine is a precursor of catecholamines, thyroxine and tyramine. The distribution and metabolic fates of tyrosine have been extensively studied, but not satisfactorily in the whole-body. In the meantime, whole-body autoradiography has been used for investigation of the distributions of hexoses (13, 21) and hexosamines (7,8) in mice in our laboratory. In the recent report (9), the distribution of radioactive tryptophan and/or its metabolites in mice was examined by this method. To our knowlege, the distribution of tyrosine in the whole body has not been reported by this method, although those of the amino acids such as methionine (1), glutamine (3) and other amino acids (12, 15) and dopa (22) have been studied. The present report deals with the tissue distribution in mice by means of whole-body autoradiography and biochemical analysis at several intervals after injection of L-[U-14C]tyrosine. MATERIALS AND METHODSAnimals. Adult male albino mice of ICR strain, weighing approx. 30 g, were used. Materials. L-[U-14C]tyrosine (specific activity: 493.0 mCi/mmol) was purchased from New England Nuclear, U. S.A. The substance was lyophilyzed and dissolved in physiological saline solution (50 iCi/ml).

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Section: Discussionmentioning

confidence: 67%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

The distribution of radioactive carbon from L-(U-14C)tyrosine in mice.

Goto

Matsushima

Kanayama

et al. 1987

Acta Histochem. Cytochem

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“…After developing and drying, horizontal scratches were made in the silica gel on the plate 3 mm apart, and the radioactivity was measured with an Aloka liquid scintillation counter (6,27). Radioactivity of each peak on the chromatogram was corrected with the original radioactivity (cpm/gram of wet weight) of the acid-soluble fraction of each organ.…”

Section: Methodsmentioning

confidence: 99%

Tissue distribution and metabolic fate of 35S from 35S-taurine in pregnant mice by whole-body autoradiographic and biochemical study.

Shimono

Shimada

1988

Acta Histochem. Cytochem

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Tissue distribution and metabolic fate of HS from 33S-taurine in pregnant mice were studied by whole-body autoradiography and biochemical analysis.Whole-body autoradiography was performed at 30 min, 3 and 6 hr after intravenous injection of 35S-taurine. In the maternal body, high densities were observed in the kidney, liver, bile and digestive tract, while low densities were in the blood and brain. The placenta showed a relatively high density, but the fetus was low density.As for the biochemical analysis, the mice were decapitated at the same intervals after injection as those for autoradiography and various tissues were removed. These tissues were fractionated into 6% perchloric acid-soluble, -insoluble and lipid fractions. Total radioactivities (cpm) per wet weight (g) were high in the liver, small intestine and kidney and low in the brain, blood and fetus. These data were consistent with those of whole-body autoradiographs. The greatest part (over 98%) of total radioactivities in all tissues was incorporated into the acid-soluble fraction.The acid-soluble fraction of each tissue was analyzed by thin-layer chromatography.The radioactive spots were examined for taurine and taurocholic acid in the bile, but only for taurine in all other tissues.

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Autoradiographic distribution of radioactivity from14C-GABA in the mouse

Kuroda

Watanabe

Tamayama

et al. 2000

Microsc. Res. Tech.

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We investigated the distribution of radioactivity from 14C‐labeled γ‐aminobutyric acid (GABA) in the mouse by in vivo autoradiography to clarify the tissues that show GABA uptake and/or GABA binding. Male mice were injected intravenously with 14C‐GABA in both the absence and presence of an excess of unlabeled GABA, baclofen and isoguvacine. Whole‐body autoradiography of 3H‐baclofen, a GABAB receptor agonist was also performed. At short intervals after 14C‐GABA injection ( 3 and 6 minutes), very high radioactivity was detected in the kidney cortex, liver, pineal gland, hypophysis, median eminence of the hypothalamus, and cervical ganglion. The hyaline cartilage and glandular part of the stomach showed moderate radioactivity. In the presence of an excess amount of unlabeled GABA, radioactivity in most of tissues decreased significantly, but no significant difference in radioactivity was observed in the presence of baclofen and isoguvacine, agonists of GABAA and GABAB receptors, respectively. Autoradiography of 3H‐baclofen showed that the kidney had high level of radioactivity, whereas the activity in other tissues and organs was similar or lower than in the blood except for the content of the urinary bladder and the pancreas at 15 minutes after injection. These data indicate that radioactivity from incorporated 14C‐GABA into a variety of cells is much higher than that from bound 14C‐GABA to the receptor sites. Our results suggest that GABA can be quickly localized in many organs of the mouse body after 3 minutes following injection, and GABA may serve multiple functions in those organs. Microsc. Res. Tech. 41:116–126, 2000. © 2000 Wiley‐Liss, Inc.

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The distribution of radioactive carbon from (U-14C)glucosamine in mice.

Cited by 11 publications

References 23 publications

The distribution of radioactive carbon from L-(U-14C)tyrosine in mice.

The distribution of radioactive carbon from L-(U-14C)tyrosine in mice.

Tissue distribution and metabolic fate of 35S from 35S-taurine in pregnant mice by whole-body autoradiographic and biochemical study.

Autoradiographic distribution of radioactivity from14C-GABA in the mouse

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