The distribution of phosphorus and its transformations during batch growth of Synechocystis

Zhou, Yun; Nguyen, Binh T.; Zhou, Chen; Straka, Levi; Lai, YenJung Sean; Xia, Siqing; Rittmann, Bruce E.

doi:10.1016/j.watres.2017.06.017

Cited by 73 publications

(29 citation statements)

References 35 publications

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“…Soluble TOC represents total organic matters and UV 254 reflects the humic acid-like macromolecular matters and aromatic compounds containing CC and CO, both of which could be used as the substrate and electron donor for heterotrophs. 18,19 dramatically increased with the increased CAPB dosage, which was consistent with the biopolymers release after addition of CAPB in Figure 1. Throughout the digestion process without addition of CAPB, both UV 254 and STOC gradually decreased from 0 to 8 h, which should be attributed to the fact that soluble biopolymers could be the electron-donor substrates and excellent carbon sources for heterotrophs.…”

Section: ■ Results and Discussionsupporting

confidence: 84%

“…Throughout the digestion process without addition of CAPB, the concentration of soluble PO 4 3− -P decreased gradually, which should result from the accumulation of phosphorus inside the cell and EPS adsorption due to the functional groups of quaternary ammonium (−NH 3 + ). 19 After addition of CAPB, soluble PO 4…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Soluble TOC represents total organic matters and UV 254 reflects the humic acid-like macromolecular matters and aromatic compounds containing CC and CO, both of which could be used as the substrate and electron donor for heterotrophs. , Figure presents the UV 254 and STOC of soluble organic matters after addition of various dosages of CAPB at different digestion times. Both STOC and UV 254 dramatically increased with the increased CAPB dosage, which was consistent with the biopolymers release after addition of CAPB in Figure .…”

Section: Resultsmentioning

confidence: 99%

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Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Zhou

Zhang

et al. 2018

ACS Sustainable Chem. Eng.

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Cocoamidopropyl betaine (CAPB) has newly been found to improve the reduction of waste-activated sludge (WAS) by a short-time aerobic digestion (STAD) process. This work systematically discloses the influences of CAPB dosage on the reduction of WAS by the STAD process. Results showed that CAPB lower than 0.10 g/g of TSS (total suspended solids) mainly increase the organic substances release and low-molecularweight (MW) fractions proportion via its surfactant action, leading to the increased concentration of soluble biopolymers. The concentration of soluble PO 4 3− -P and NH 4 + -N increased during the biopolymers release, but gradually decreased in the later stage. Moreover, the specific oxygen uptake rate (SOUR) of digested sludge (denoted as aerobic microorganisms activity) was increased, resulting in the increased biodegradation rate of WAS chemical oxygen demand (k COD,WAS ). A higher amount of CAPB (>0.10 g/g of TSS) led to cell lysis and intracellular polymeric substances (IPS) release, which led to an additional increase of PO 4 3− -P, NH 4 + -N, soluble organic matters, and its low MW fractions; all these fractions gradually decreased later. Nevertheless, cell lysis reduces both pH value and SOUR, which caused the decrease of CAPB biodegradation rate and k COD,WAS . This study lays the foundation for improving the reduction efficiency of WAS in the STAD system by optimizing surfactant dose.

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Section: ■ Results and Discussionsupporting

confidence: 84%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Zhou

Zhang

et al. 2018

ACS Sustainable Chem. Eng.

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“…Thus, phosphate deprivation is unlikely to be the main reason for the cessation of growth after six days in wastewater containing 8 times more phosphate than in BG-11-5%P. In cyanobacteria, phosphate taken from growth medium is deposited to an inner cellular pool and to an extracellular pool [42][43][44]. Cells first both take in phosphate and adsorb it with the extracellular matrix, and when the growth medium is depleted of phosphate, phosphate from the extracellular matrix is taken in [44].…”

Section: Discussionmentioning

confidence: 99%

“…In cyanobacteria, phosphate taken from growth medium is deposited to an inner cellular pool and to an extracellular pool [42][43][44]. Cells first both take in phosphate and adsorb it with the extracellular matrix, and when the growth medium is depleted of phosphate, phosphate from the extracellular matrix is taken in [44]. Inside the cells, phosphate is stored as polyphosphate [17], and Synechocystis has been speculated to use multiple DNA copies as a phosphate storage as well, as at least the number of genome copies is dependent on the amount of phosphate [45].…”

Section: Discussionmentioning

confidence: 99%

Testing the Potential of Regulatory Sigma Factor Mutants for Wastewater Purification or Bioreactor Run in High Light

et al. 2020

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It is shown that a freshly inoculated culture of the model cyanobacterium Synechocystis sp. PCC 6803 consumed almost all phosphate and 50% of nitrate within 6 days from the nutrient-rich BG-11 growth medium, indicating potential of cyanobacteria to purify wastewaters. Synechocystis sp. PCC 6803 control strain also collected nutrients efficiently from a landfill leachate wastewater KA2 (5.9-6.9 mM ammonium and 0.073-0.077 mM phosphate). Wastewaters might induce oxidative stress to microalgae, which prompted us to test growth of sigma factor inactivation strains, as ΔsigBCE and ΔsigCDE strains show superior growth in chemically induced oxidative stress. All cyanobacterial strains, including a stress-sensitive strain ΔsigBCDE, grew well in KA2 for four days, indicating that KA2 did not cause immediate oxidative stress. Completely arrested growth and bleaching of ΔsigBCDE cells after one week in KA2 wastewater point to the importance of group 2 sigma factor-mediated changes in gene expression during wastewater treatment. The growth of ΔsigBCD was arrested early in un-buffered and Hepes buffered (pH 7.5) KA2. In ΔsigBCD, all phosphate transporter genes are upregulated in standard conditions, and ΔsigBCD cells showed growth defects in low-phosphate BG-11 medium. ΔsigBCD cells removed phosphate slower from KA2 than the control strain, but phosphate supplementation of KA2 did not improve growth of ΔsigBCD. The ΔsigBCE strain showed superior growth in a laboratory-scale bioreactor in bright light and removed phosphate even slightly more efficiently than the control strain if KA2 was Hepes buffered although ΔsigBCE grew slowly in un-buffered KA2 and in low-phosphate BG-11 medium. The results indicate that engineering expression of regulatory group 2 sigma factor(s) might be useful for practical applications.

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Phosphate depletion controls lipid content and accumulation of heterotrophic bacteria during growth of Synechocystis sp. PCC 6803

Zhou

Lai

Eustance

et al. 2019

Appl Microbiol Biotechnol

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The distribution of phosphorus and its transformations during batch growth of Synechocystis

Cited by 73 publications

References 35 publications

Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Cocoamidopropyl Betaine Dosage Dependence of Short-Time Aerobic Digestion for Waste-Activated Sludge Reduction

Testing the Potential of Regulatory Sigma Factor Mutants for Wastewater Purification or Bioreactor Run in High Light

Phosphate depletion controls lipid content and accumulation of heterotrophic bacteria during growth of Synechocystis sp. PCC 6803

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