The distribution of methionine loci in Neurospora crassa

Murray, Noreen E.

doi:10.1038/hdy.1960.76

Cited by 26 publications

(6 citation statements)

References 16 publications

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“…This is illustrated in Figure 6, where the model correctly predicts that cys-5 , cys-9 , and cys-11 mutants can be rescued when the downstream nutrients sulfite and thiosulfate are provided in the media [85]. Similarly, the model correctly predicts that met-2 , met-5 , met-6 , met-7 and met-8 mutants are rescued by L-methionine; met-2 , met-5 and met-7 mutants are rescued by L-homocysteine; and met-5 and met-7 mutants are rescued by L-cystathione [86], [87].…”

Section: Resultsmentioning

confidence: 93%

Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM

et al. 2013

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The filamentous fungus Neurospora crassa played a central role in the development of twentieth-century genetics, biochemistry and molecular biology, and continues to serve as a model organism for eukaryotic biology. Here, we have reconstructed a genome-scale model of its metabolism. This model consists of 836 metabolic genes, 257 pathways, 6 cellular compartments, and is supported by extensive manual curation of 491 literature citations. To aid our reconstruction, we developed three optimization-based algorithms, which together comprise Fast Automated Reconstruction of Metabolism (FARM). These algorithms are: LInear MEtabolite Dilution Flux Balance Analysis (limed-FBA), which predicts flux while linearly accounting for metabolite dilution; One-step functional Pruning (OnePrune), which removes blocked reactions with a single compact linear program; and Consistent Reproduction Of growth/no-growth Phenotype (CROP), which reconciles differences between in silico and experimental gene essentiality faster than previous approaches. Against an independent test set of more than 300 essential/non-essential genes that were not used to train the model, the model displays 93% sensitivity and specificity. We also used the model to simulate the biochemical genetics experiments originally performed on Neurospora by comprehensively predicting nutrient rescue of essential genes and synthetic lethal interactions, and we provide detailed pathway-based mechanistic explanations of our predictions. Our model provides a reliable computational framework for the integration and interpretation of ongoing experimental efforts in Neurospora, and we anticipate that our methods will substantially reduce the manual effort required to develop high-quality genome-scale metabolic models for other organisms.

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Section: Resultsmentioning

confidence: 93%

Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM

et al. 2013

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“…The media were as previously described (Murray, 1960) except that in plating experiments the sorbose concentration was increased to so per cent.…”

Section: Methodsmentioning

confidence: 99%

Complementation and recombination between Methionine-2 alleles in Neurospora crassa

Murray

1960

Heredity

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AMONGST the many new methionine mutant strains of Jt[eurospora crassa which have been isolated (Murray, 1960) the sulphur requirement of forty-two is satisfied by homocysteine or methionine, but not by cystathionine. Heterocaryon tests show that the mutant genes in these strains are physiologically allelic with H98 and 48004 (me-2) and that some pairs of them exhibit the phenomenon of interallelic complementation. Methionine-2 is located in• the right arm of linkage group IV, in a region well supplied with genetic markers, and as interallelic crosses proved to be relatively fertile, it appeared that this gene would be suitable for the investigation of the relationship between genetic and complementation maps. The data from ordered tetrads (Murray, 1960) indicated that me-2 is 87 units distal to typ-4 and 37 units proximal to pan-I. Therefore, trp-4 and pan-I were chosen as suitable markers for the analysis of recombination between me-2 alleles. The discovery of recombination between the two physiologically allelic mutants "Star" and "asteroid" in Drosophila (Lewis, 1945), and more recently between alleles at loci in Aspergillus (Pritchard, 1955), JVeurospora (Giles, 1951, 1956; Mitchell, 1955a, I.955b) and yeasts (Roman, 1956; Leupold, 1957), suggests that the gene may be interpreted as comprising a number of linearly arranged mutable sites which are separable by crossing-over. Pritchard (1955) was able to arrange a number of adenine alleles of Aspergillus in linear order by an analysis controlled by markers on each side of the locus. In general, recombination between the adenine alleles was accompanied by recombination of the associated markers. Prototroph formation was therefore explicable as the result of a single cross-over between the defective sites of the locus. Such a cross-over would produce one normal and one doubly mutant chromatid strand, and Pritchard was able to recover the doubly mutant recombinant. Other adenine prototrophs, of parental phenotype with respect to the marker genes, could not be interpreted as the products of single intragenic cross-overs, but were, perhaps, comparable in origin with the prototrophs from crosses of allelic pyridoxine mutants of .J'Teurospora (Mitchell, 1955a, i955b). By studies of ordered tetrads Mitchell showed that * Work done while holder of a postgraduate grant from the

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“…Uses cystathionine, homocysteine, or methionine (354,718). Defective homosenne transacetylase (547, 733) (Fig.…”

Section: In(il -) Ir)h4250mentioning

confidence: 99%

“…Between ff-S (1 to 4%) and ad4 (4%) (219, 815, 1052). ( 718) Uses methionine but not precursors (718). Lacks methyl tetrahydrofolate homocysteine transmethylase (124, 964) (Fig.…”

Section: In(il -) Ir)h4250mentioning

confidence: 99%

Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

259

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I I ' ~~~~T h Ñ CiOr(r r N z ADDITIONAL LOCI IN LINKAGE GROUP ILinked to mt (8 to 12%) Linked to mt, acr-3 (5%) Right of arg-i (5/29) Linked to so and aro-8 Right of un-18 Between ad-5 and the centromere Between arg-i (4%) and his-3 (I to 2%) Between In(H4250) and T(39311) Left of mt (23%) Linked to mt (2%) and aza-i (39%o) Left of nit-2 (30%) Left of mt (14%) Linked to un-i (9%), probably to the right Linked to hom (1%), probably to the right Linked to mt (17 to 22%) Between T(NM103) and al-2 (18%) Between T(4540) and cr-2Between thi-i (12 to 20%/6) and ad-9 (5%) Linked to ad-5 (1/54), probably to the left Linked to mt (6%) Linked to cys-5 (<1%), probably to the right Right of ad-9 (12%), linked to al (0/76) Right of his-3 (2%) Between the T(AR173) breakpoints Linked to al-2 (10%) and nic-i (1 to 5%) Linked to ad-9 (0/44); right of thi-i (2%) Linked to mt (5%), probably to the left Linked to al-I (10%) Right of os-i (3%) Between nit,i (5 to 19%) and al-I (6 to 19%io) Linked near arg-i Left of al-2 (25%o) Left of mt (16%) Right of nic-2 (6%) Linked to ad-9 (2%), probably to the right Left of cr-i (15%) Left of mt (10%) Probably between his-2 (3%) and cr-i (3%) Between T(NM103) and the right tip Between mt (20%) and arg-i (1%) Between ad-3 (6%) and al-i (16%) Between his-3 and nic-2 Between cyh-i (5%) and al-I (7%) Linked to cyh-i (18%), al-i (2%), and mb-2 (6%) Between arg-i (3%) and T(39311) Between tre (41%) and ad-9 Linked to mt (9%) Linked to mt (20%) Complex phenotype. Alternative requirements: 2-acetylaminofluorine, certain azo dyes, or certain single amino acids. Cold sensitive. Originated among progeny of a rib-i strain that had become tolerant to 2-acetylaminofluorine.

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The distribution of methionine loci in Neurospora crassa

Cited by 26 publications

References 16 publications

Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM

Reconstruction and Validation of a Genome-Scale Metabolic Model for the Filamentous Fungus Neurospora crassa Using FARM

Complementation and recombination between Methionine-2 alleles in Neurospora crassa

Chromosomal loci of Neurospora crassa

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