The distribution of lead in duckweed (Lemna minor L.) root tip

Samardakiewicz, Sławomir; Woźny, Adam

doi:10.1007/978-94-017-2858-4_19

Cited by 12 publications

(19 citation statements)

References 13 publications

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“…Accumulation of heavy metal ions in roots is a result of the function of detoxicating mechanisms that consist in the following: metal binding on the root surface and its binding in the cell wall, chelating in the cytoplasm, transport of metal complexes by the tonoplast and their depositing in the vacuoles. Many studies showed that cell wall (Samardakiewicz and Wozny 2000;Seregin et al 2002) and apoplast (Seregin et al 2002) are the main sites of Pb accumulation. This is an additional defence barrier protecting cell metabolism against toxic effects.…”

Section: Discussionmentioning

confidence: 98%

“…However, part of Pb penetrated the cell and its presence was localized in various cell compartments. The occurrence of Pb was observed in the vacuoles, mitochondria and peroxisomes (Samardakiewicz and Wozny 2000;Jarvis and Leung 2001). Pb occurrence was reported in the endoplasmic reticulum (Jarvis and Leung 2001) and nuclear membrane and in the cell membrane (Samardakiewicz and Wozny 2000).…”

Section: Discussionmentioning

confidence: 98%

“…Small amounts of Pb enter cytosol, partially accumulate in dictyosomes and then they are transported with dictyosomal vesicles back to cell wall (Samardakiewicz and Wozny 2000). Numerous studies indicate that lead penetrates root cells and is translocated there via the apoplastic pathway, using the transpiration conductive system (Tung and Temple 1996;Wierzbicka 1999).…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of lead in root cells of Pisum sativum

Małecka

Piechalak

Morkunas³

et al. 2008

Acta Physiol Plant

119

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The ever-increasing environmental pollution necessitates organisms to develop specific defense systems in order to survive and function effectively. Lead is taken up by plants mainly through roots and over 96% are accumulated there.Pea plants were cultivated hydroponically for 4 days with 0.1, 0.5 and 1 mM Pb(NO 3 ) 2 . Uptake of lead ions from nutrient solution and accumulation in root stems and leaves during 96-h cultivation was estimated. The root tip cells were observed with transmission electron microscope to analyse their ultrastructure and lead localization. Pb was accumulated in the cell wall, cell membrane, vacuoles, mitochondria and peroxisomes. The fractions of mitochondria and peroxisomes were isolated from pea roots purified by means Percoll gradient, and were observed by means of electron microscope with the attachment for X-ray microanalysis. Visible deposits containing Pb were observed in both cell organelles.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Accumulation of lead in root cells of Pisum sativum

Małecka

Piechalak

Morkunas³

et al. 2008

Acta Physiol Plant

119

View full text Add to dashboard Cite

show abstract

“…Moreover, the detection of TMs is possible by X-ray microanalysis (Samardakiewicz and Woźny 2000;Neumann and zur Nieden 2001;Carrier et al 2003;Kopittke et al 2008;Meyers et al 2009;Krzesłowska et al 2010), energy dispersive transmission electron microscopy (Lütz-Meindl 2007) and micro-protoninduced X-ray emission (Vogel-Mikuš et al 2008). These methods enable detection of TMs when they do not form deposits and are dissolved, e.g., in cytosol.…”

Section: Detection Of Tms In Plant Cell Wallmentioning

confidence: 99%

“…The occurrence of TMs within the CW has been reported since the earliest studies of plant cell reactions to TMs (e.g., Malone et al 1974, Woźny et al 1982Wierzbicka 1998;Samardakiewicz and Woźny 2000;Neumann and zur Nieden 2001). Recent papers indicate that the CW is still recognized as one of the main compartments for TM accumulation (e.g., Wójcik et al 2005;Islam et al 2007;Małecka et al 2008;Konno et al 2010;Meyers et al 2009) and can contain high amounts of TMs (Kopittke et al 2007(Kopittke et al , 2008Konno et al 2010;Krzesłowska et al 2009aKrzesłowska et al , 2010.…”

Section: Detection Of Tms In Plant Cell Wallmentioning

confidence: 99%

The cell wall in plant cell response to trace metals: polysaccharide remodeling and its role in defense strategy

2010

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This review paper is focused predominantly on the role of the cell wall in the defense response of plants to trace metals. It is generally known that this compartment accumulates toxic divalent and trivalent metal cations both during their uptake by the cell from the environment and at the final stage of their sequestration from the protoplast. However, from results obtained in recent years, our understanding of the role played by the cell wall in plant defense response to toxic metals has markedly altered. It has been shown that this compartment may function not only as a sink for toxic trace metal accumulation, but that it is also actively modified under trace metal stress. These modifications lead to an increase in the capacity of the cell wall to accumulate trace metals and a decrease of its permeability for trace metal migration into the protoplast. One of the most striking alterations is the enhancement of the level of low-methylesterified pectins: the polysaccharides able to bind divalent and trivalent metal ions. This review paper will present the most recent results, especially those that are concerned with polysaccharide level, composition and distribution under trace metal stress, and describe in detail the polysaccharides responsible for metal binding and immobilization in different groups of plants (algae and higher plants). The review also contains information related to the entry pathways of trace metals into the cell wall and their detection methods.

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Lead toxicity to Lemna minor predicted using a metal speciation chemistry approach

Antunes

Kreager²

2014

Enviro Toxic and Chemistry

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In the present study, predictive measures for Pb toxicity and Lemna minor were developed from bioassays with 7 surface waters having varied chemistries (0.5-12.5 mg/L dissolved organic carbon, pH of 5.4-8.3, and water hardness of 8-266 mg/L CaCO3 ). As expected based on water quality, 10%, 20%, and 50% inhibitory concentration (IC10, IC20, and IC50, respectively) values expressed as percent net root elongation (%NRE) varied widely (e.g., IC20s ranging from 306 nM to >6920 nM total dissolved Pb), with unbounded values limited by Pb solubility. In considering chemical speciation, %NRE variability was better explained when both Pb hydroxides and the free lead ion were defined as bioavailable (i.e., f{OH} ) and colloidal Fe(III)(OH)3 precipitates were permitted to form and sorb metals (using FeOx as the binding phase). Although cause and effect could not be established because of covariance with alkalinity (p = 0.08), water hardness correlated strongly (r(2) = 0.998, p < 0.0001) with the concentration of total Pb in true solution ([Pb]T_True solution ). Using these correlations as the basis for predictions (i.e., [Pb]T_True solution vs water hardness and %NRE vs f{OH} ), IC20 and IC50 values produced were within a factor of 2.9 times and 2.2 times those measured, respectively. The results provide much needed effect data for L. minor and highlight the importance of chemical speciation in Pb-based risk assessments for aquatic macrophytes.

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The distribution of lead in duckweed (Lemna minor L.) root tip

Cited by 12 publications

References 13 publications

Accumulation of lead in root cells of Pisum sativum

Accumulation of lead in root cells of Pisum sativum

The cell wall in plant cell response to trace metals: polysaccharide remodeling and its role in defense strategy

Lead toxicity to Lemna minor predicted using a metal speciation chemistry approach

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